High diversity of hemotropic mycoplasmas in Iberian wild carnivores

Millán, Javier; Velarde, Roser; Delicado, Verónica; Negre, Nieves; Ribas, Alexis; Oleaga, Álvaro; Llaneza, Luís; Esperón, Fernando

doi:10.1016/j.cimid.2018.09.007

Cited by 12 publications

(21 citation statements)

References 34 publications

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“…The hemoplasma prevalence of 24% in guigna was within the range described for other species of solitary wild felids 22–26 and wild carnivores worldwide 28 . The highest prevalence reported for a solitary carnivore was for the Darwin’s fox in southern Chile 41 (57%).…”

Section: Discussionsupporting

confidence: 69%

“…Both were infected by Mhf and one was coinfected with C Mhm. In domestic cats, Mhf has been described as the most pathogenic hemoplasma species 11–13,28 , which may explain our findings in guignas. However, Mhf -infected guignas not showing clinical signs were also found.…”

Section: Discussionsupporting

confidence: 59%

“…b . euptilurus ), European wildcat ( Felis silvestris silvestris ), several Brazilian felid species, Iriomote cat ( Prionailurus bengalensis iriomotensis ) and African lion ( Panthera leo ) 22–28 . Hemoplasma sequences from wildlife species often share close identity to those found in domestic cats 23,25,27 .…”

Section: Introductionmentioning

confidence: 99%

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Assessing cross-species transmission of hemoplasmas at the wild-domestic felid interface in Chile using genetic and landscape variables analysis

Sacristán

Acuña

Aguilar

et al. 2019

Sci Rep

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The co-occurrence of domestic cats (Felis silvestris catus) and wild felids in rural landscapes can facilitate pathogen transmission. However, in the relatively-isolated regions of southern South America there have been no comprehensive studies to assess disease transmission risks between domestic cats and forest-dwelling wild felids such as guigna (Leopardus guigna). We evaluated hemoplasma infection and the possibility of transmission between domestic cats and guignas by comparing spatial and phylogenetic patterns of pathogen prevalence. Blood/spleen samples were collected from 102 wild guignas and 262 co-occurring rural domestic cats across the entire distribution range of guigna in Chile. Hemoplasma infection was assessed by direct sequencing of the 16S RNA gene. Infection with hemoplasmas was common and geographically widespread across different bioclimatic areas for both species. The most common feline Mycoplasma species in guigna and domestic cats were Candidatus M. haemominutum (CMhm) (15.7% guigna; 10.3% domestic cat) and Mycoplasma haemofelis (Mhf) (9.8% guigna, 6.1% domestic cat). A previously undescribed Mycoplasma sp. sequence was found in two guignas and one cat. Continuous forest-landscapes were associated with higher hemoplasma-prevalence in guignas. Shared hemoplasma nucleotide sequence types between guigna and domestic cats were rare, suggesting that cross-species transmission between guignas and domestic cats may occur, but is probably uncommon. Ectoparasites, which have been linked with hemoplasma transmission, were not found on guignas and were infrequent on domestic cats. Our results suggest that transmission pathways vary among hemoplasma species and, contrary to our predictions, domestic cats did not appear to be the main driver of hemoplasma infection in guignas in these human-dominated landscapes.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionsupporting

confidence: 59%

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Assessing cross-species transmission of hemoplasmas at the wild-domestic felid interface in Chile using genetic and landscape variables analysis

Sacristán

Acuña

Aguilar

et al. 2019

Sci Rep

Self Cite

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“…Our novel assays detailed herein may not only be a useful diagnostic method for use in a veterinary context but also for pulling apart canine VBP transmission dynamics in other relevant lifecycle hosts, such as arthropod vectors or non-domesticated wildlife. For example, our qPCRs could be used to test samples from wild canids that are known to maintain reservoirs of some VBPs [73,74], whilst a modification to the assay's DNA extraction control to detect invertebrate mtDNA could allow the qPCRs to surveil for canine VBP prevalence in collected tick and flea vectors as well.…”

Section: Discussionmentioning

confidence: 99%

Novel High-Throughput Multiplex qPCRs for the Detection of Canine Vector-Borne Pathogens in the Asia-Pacific

et al. 2021

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The Asia-Pacific hosts a large diversity of canine vector-borne pathogens (VBPs) with some of the most common and most pathogenic, generating significant mortality as well as a spectrum of health impacts on local dog populations. The VBPs Anaplasma platys, Babesia gibsoni, Babesia vogeli, Ehrlichia canis, Hepatozoon canis and haemotropic Mycoplasma spp. are all endemic throughout the region, with many exhibiting shifting geographical distributions that warrant urgent attention. Moreover, many of these species cause similar clinical signs when parasitising canine hosts, whilst knowledge of the exact pathogen is critical to ensure treatment is effective. This is complicated by frequent coinfection that can exacerbate pathology. Here, we describe the development, optimisation and validation of two novel quadruplex Taq-Man based real-time PCRs (qPCRs) for the specific and sensitive detection of the aforementioned VBPs. To ensure accurate evaluation of diagnostic performance, results of our qPCRs were evaluated on field samples from Thai dogs and compared with both conventional PCR (cPCR) results and next-generation sequencing (NGS) metabarcoding. Our qPCRs were found to be more sensitive at detecting canine VBP than cPCR and generated results similar to those achieved by NGS. These qPCRs will provide a valuable high-throughput diagnostic tool available to epidemiologists, researchers and clinicians for the diagnosis of key canine VBPs in the Asia-Pacific and further afield.

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“…There is a lack of information about mycoplasma in wild animals. Moreover, only a few studies report the occurrence of mycoplasmas in red foxes (Kanamoto et al, 1981;Sasaki et al, 2008;Koneval et al, 2017;Millán et al, 2018). Mycoplasma spp.…”

Section: 8s-its2-28smentioning

confidence: 99%

Pathogen screening in the red fox (Vulpes Vulpes) from Lithuania

Paulauskas¹,

Sakalauskas²,

Lipatova³

et al. 2020

biologija

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The aim of this study was to screen free-ranging red foxes from Lithuania for the presence of different vector-borne pathogens. A total of 31 red foxes from three districts of Lithuania were molecularly tested for the presence of pathogens. Five different pathogens were detected in 83.9% of red foxes: Anaplasma spp., Bartonella spp., Rickettsia spp., Borrellia spp. and Babesia spp. The presence of Mycoplasma spp. and Dirofilaria spp. was not detected in our study.

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High diversity of hemotropic mycoplasmas in Iberian wild carnivores

Cited by 12 publications

References 34 publications

Assessing cross-species transmission of hemoplasmas at the wild-domestic felid interface in Chile using genetic and landscape variables analysis

Assessing cross-species transmission of hemoplasmas at the wild-domestic felid interface in Chile using genetic and landscape variables analysis

Novel High-Throughput Multiplex qPCRs for the Detection of Canine Vector-Borne Pathogens in the Asia-Pacific

Pathogen screening in the red fox (Vulpes Vulpes) from Lithuania

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