High discrimination of Mycobacterium bovis isolates in Brazilian herds by spoligotyping

Rocha, Vivianne Cambuí Figueiredo; Filho, Antônio Francisco de Souza; Ikuta, Cássia Yumi; Filho, José Henrique Hildebrand e Grisi; Issa, Marina de Azevedo; Mota, Pedro; Ferré, Lucía de Juan; Rodríguez, Lucas Domínguez; Romero, Beatriz; Heinemann, Marcos Bryan; Neto, José Soares Ferreira

doi:10.1016/j.prevetmed.2020.104976

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…Since B. indicus cattle are phylogenetically more ancient than B. taurus cattle with respect to their common ancestor B. primigenius ( 67 ), our results suggest that BS cattle are immunologically potentially more closely related to ancient cattle breeds such as Boran and Sahiwal than the HF breed. Whether there is a co-evolution of different M. bovis spoligotypes with the bovine host is not yet know, but recent data suggest that it is more likely based on geographical area and farming practises rather than the host ( 68 – 70 ).…”

Section: Discussionmentioning

confidence: 99%

Single Nucleotide Polymorphisms in the Bovine TLR2 Extracellular Domain Contribute to Breed and Species-Specific Innate Immune Functionality

et al. 2021

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Recent evidence suggests that several cattle breeds may be more resistant to infection with the zoonotic pathogen Mycobacterium bovis. Our data presented here suggests that the response to mycobacterial antigens varies in macrophages generated from Brown Swiss (BS) and Holstein Friesian (HF) cattle, two breeds belonging to the Bos taurus family. Whole genome sequencing of the Brown Swiss genome identified several potential candidate genes, in particular Toll-like Receptor-2 (TLR2), a pattern recognition receptor (PRR) that has previously been described to be involved in mycobacterial recognition. Further investigation revealed single nucleotide polymorphisms (SNP) in TLR2 that were identified between DNA isolated from cells of BS and HF cows. Interestingly, one specific SNP, H326Q, showed a different genotype frequency in two cattle subspecies, Bos (B.) taurus and Bos indicus. Cloning of the TLR2 gene and subsequent gene-reporter and chemokine assays revealed that this SNP, present in BS and Bos indicus breeds, resulted in a significantly higher response to mycobacterial antigens as well as tri-acylated lipopeptide ligands in general. Comparing wild-type and H326Q containing TLR2 responses, wild-type bovine TLR2 response showed clear, diminished mycobacterial antigen responses compared to human TLR2, however bovine TLR2 responses containing H326Q were found to be partially recovered compared to human TLR2. The creation of human:bovine TLR2 chimeras increased the response to mycobacterial antigens compared to the full-length bovine TLR2, but significantly reduced the response compared to the full-length human TLR2. Thus, our data, not only present evidence that TLR2 is a major PRR in the mammalian species-specific response to mycobacterial antigens, but furthermore, that there are clear differences between the response seen in different cattle breeds, which may contribute to their enhanced or reduced susceptibility to mycobacterial infection.

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Section: Discussionmentioning

confidence: 99%

Single Nucleotide Polymorphisms in the Bovine TLR2 Extracellular Domain Contribute to Breed and Species-Specific Innate Immune Functionality

et al. 2021

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“…Genetic differentiation of M. bovis strains can provide better understanding about the evolution, epidemiology and ecology of bTB [24][25][26][27]. The molecular tools currently used worldwide for genotyping M. bovis isolates are spoligotyping and variable number tandem repeat (VNTR) analysis [28][29][30][31]. However, although these genotyping methods have proven useful in identifying local clusters, there are limitations regarding their ability to discriminate events within clusters involved in persistence and in assessing the spread of bTB [32,33].…”

Section: Introductionmentioning

confidence: 99%

Genomic and temporal analyses of Mycobacterium bovis in southern Brazil

et al. 2021

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Mycobacterium bovis is a causal agent of bovine tuberculosis (bTB), one of the most important diseases currently facing the cattle industry worldwide. Tracing the source of M. bovis infections of livestock is an important tool for understanding the epidemiology of bTB and defining control/eradication strategies. In this study, whole genome sequencing (WGS) of 74 M . bovis isolates sourced from naturally infected cattle in the State of Rio Grande do Sul (RS), southern Brazil, was used to evaluate the population structure of M. bovis in the region, identify potential transmission events and date the introduction of clonal complex (CC) European 2 (Eu2). In silico spoligotyping identified 11 distinct patterns including four new profiles and two CCs, European 1 (Eu1) and Eu2. The analyses revealed a high level of genetic diversity in the majority of herds and identified putative transmission clusters that suggested that within- and between-herd transmission is occurring in RS. In addition, a comparison with other published M. bovis isolates from Argentina, Brazil, Paraguay and Uruguay demonstrated some evidence for a possible cross-border transmission of CC Eu1 into RS from Uruguay or Argentina. An estimated date for the introduction of CC Eu2 into RS in the middle of the 19th century correlated with the historical introduction of cattle into RS to improve existing local breeds. These findings contribute to the understanding of the population structure of M. bovis in southern Brazil and highlight the potential of WGS in surveillance and helping to identify bTB transmission.

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Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil

Souza

Lopes

Silva

et al. 2022

Trop Anim Health Prod

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High discrimination of Mycobacterium bovis isolates in Brazilian herds by spoligotyping

Cited by 5 publications

References 23 publications

Single Nucleotide Polymorphisms in the Bovine TLR2 Extracellular Domain Contribute to Breed and Species-Specific Innate Immune Functionality

Single Nucleotide Polymorphisms in the Bovine TLR2 Extracellular Domain Contribute to Breed and Species-Specific Innate Immune Functionality

Genomic and temporal analyses of Mycobacterium bovis in southern Brazil

Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil

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