High dilutions of acetone affect the Avena sativa growth in vitro

Abstract

Introduction: Acetone is an organic solvent with molecular structure CH3(CO)CH3, its endogenous production in the animal body is called ketosis. The production of this compound increases with the fat. Acetone influences the lipid membrane, altering its fluidity and lipid composition [1], causing cell damage and leakage and can cause cell death. The use of herbicides in organic farming is not accepted by the Brazilian legislation [2]. So the weed control becomes a problem for organic farmers. The aim of this study is to evaluate the herbicide potential of high dilutions of acetone on Avena sativa L.
 
 Materials and Methods: The preliminary tests were conducted at the Laboratory of Plant Physiology and Homeopathy, State University of Maringá (UEM). The seeds of Avena sativa are placed in Petri dishes. Fitty seeds were germinated and grown in Petri dishes containing 15ml of high dilution of acetone and maintained at 25°C ± 2 and 12h photoperiod. Acetone dilutions (6, 12, 18, 24 and 30cH) were obtained according to the Brazilian Homeopathic Pharmacopoeia [3]. Were evaluated the shoot length (cm), total length (cm), fresh root (mg) and total dry mass (mg). The plants growth was measured after 7 days. The control consisted of distilled water. The experiment evaluated 4 replicates of each treatment and the data were analyzed by ANOVA and means were compared by Scott-Knott test (P ≤ 0.05).
 
 Results and Discussion: Dilutions 6, 24 and 30 cH inhibited the growth of the shoot and total seedling of A. sativa. The root fresh weight was significantly reduced by 4 dilutions (6,12,24 and 30x), with no difference of 24x compared to the control. The total dry mass of plants of A. sativa was reduced in all the dilutions studied, showing an inhibitory effect on growth of seedlings subjected to treatment. Somehow, acetone diluited inhibited the growth and accumulation of biomass of these seedlings, suggesting an imbalance in metabolism that resulted in a reduction in the variables values.
 
 Conclusion: The results suggest that high dilutions acetone interfere on the growth and accumulation of biomass of A. sativa.

The Effect of Centella (Centella asiatica (L.) Urb.) Extract with Several Types of Solvents as a Biostimulant on the Growth of Pagoda Mustard (Brassica rapa var. narinosa L.)

“…Pemberian ekstrak pegagan dengan pelarut aseton memberikan pengaruh yang nyata terhadap panjang akar, namun menghambat pertumbuhan tanaman sawi pagoda disebabkan karena pelarut aseton memberikan dampak yang kurang baik terhadap membran lipid, mengubah fluiditas dan komposisi lipid, menyebabkan kebocoran sel hingga berujung pada kematian sel (Reis, 2011). Berdasarkan penelitaan yang dilakukan Banu, Roberto dan Taolin (2015) bahwa tanaman sawi yang diberikan ekstrak daun kelor dan diekstraksi dengan akuades belum dapat meningkatkan panjang akar secara signifikan.…”