High Deoxyribonucleic Acid Content of Spermatozoa from Infertile, Oligospermic Human Males

Frajese, Gaetano; Silvestroni, L.; Malandrino, F.; Isidori, Aldo

doi:10.1016/s0015-0282(16)41590-6

Cited by 11 publications

(3 citation statements)

References 6 publications

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“…Sperm chromatin is highly compacted during spermatogenesis by the replacement of nuclear histones with protamines. Protamines are basic proteins containing numerous cysteine and arginine residues that are present in small quantities in the immature germ cells (Frajese, Silvestroni, Malandrino, & Isidori, 1976). This unique packaging allows sperm cells to maintain compact structure, which confers stability, and occupies almost the total nucleus volume.…”

Section: Abnormal Chromatin Compactionmentioning

confidence: 99%

TUNEL assay—Standardized method for testing sperm DNA fragmentation

et al. 2020

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Sperm DNA integrity is important for normal functions such as fertilization, implantation, pregnancy and fetal development. Sperm DNA fragmentation (SDF) is more common in infertile men and may be responsible for poor reproductive function. Although there are a number of tests available to measure SDF, the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling TUNEL) assay using flow cytometry is becoming more popular to measure the sperm DNA fragmentation. It is a direct test that measures both single-and double-DNA strand breaks. In this review, we describe the protocol, quality control and measurement of sperm DNA fragmentation using a benchtop flow cytometer. We also briefly discuss the factors that can affect the results, challenges and clinical implications of TUNEL in assessing male infertility.

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Section: Abnormal Chromatin Compactionmentioning

confidence: 99%

TUNEL assay—Standardized method for testing sperm DNA fragmentation

et al. 2020

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“…Attempts have been made to compare the DNA contents of sperm obtained from normal and infertile individuals with different percentages of head-shape abnormalities, but these studies have been inconclusive [7][8][9][10]. One hypothesis is that the shape of the head might be affected to some extent by the volume of material that is packed inside it.…”

Section: Introductionmentioning

confidence: 99%

Atomic Force Microscope Analysis of Chromatin Volumes in Human Sperm with Head-Shape Abnormalities1

Lee

Allen

Balhorn

1997

Biology of Reproduction

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We used the atomic force microscope to perform volume measurements on individual human sperm nuclei under ambient conditions. Data obtained for normal sperm and for sperm from seven of the nine classes of head-shape abnormality revealed that the nuclear volumes were essentially identical for sperm in each headshape class, even though the projected areas and shapes of the nuclei have were shown to vary widely. These results indicate that the abnormal sperm head morphologies found at a rate of 25-40% in fertile males are not caused by factors that affect the volume of sperm chromatin, such as the DNA content of the sperm nucleus, differences in chromatin organization, or the extent of DNA compaction.

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“…However, variabilities in DNA contents of spermatozoa have been reported [5,9,12-161 suggesting that they might be associated with fertility impairments and sperm cell aging [I].…”

Section: Introductionmentioning

confidence: 99%

Deoxyribonuclease Activity in Human Seminal Fluid

Sagiv

Allalouf

et al. 1983

Archives of Andrology

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Deoxyribonuclease (DNase) activity was examined in the whole and two split fractions of human seminal fluid from normospermic, oligozoospermic, and azoospermic origins as well as in sonicates of isolated sperm after freezing and thawing of samples and at various pH values of substrates. The method consisted in the measurement of digested areas in plates containing herring sperm deoxyribonucleic acid (DNA). No correlation was found between DNase activity (875 ? 22 (SE) nglml) and seminal fluid quality. The enzyme activity was significantly lower in the second split portion (764 t 43 ng/ml) as compared to the first (971 t 41 ng/ml). Sonicates of washed sperm were inactive. It is suggested that DNase activity derives from the epididymis and possibly from the vas deferens where it participates in the decomposition of DNA from dead cells.

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High Deoxyribonucleic Acid Content of Spermatozoa from Infertile, Oligospermic Human Males

Cited by 11 publications

References 6 publications

TUNEL assay—Standardized method for testing sperm DNA fragmentation

TUNEL assay—Standardized method for testing sperm DNA fragmentation

Atomic Force Microscope Analysis of Chromatin Volumes in Human Sperm with Head-Shape Abnormalities1

Deoxyribonuclease Activity in Human Seminal Fluid

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