High density oligonucleotide array analysis of interferon-α2a sensitivity and transcriptional response in melanoma cells

Certa, Ulrich; Seiler, Monika; Padovan, Elisabetta; Spagnoli, Giulio C.

doi:10.1054/bjoc.2001.1865

Cited by 46 publications

(36 citation statements)

References 36 publications

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“…In a different experimental setting, Certa et al (2000) addressed the genetic profile of melanoma cell lines classified according to their sensitivity or insensitivity to Interferon-a treatment. This study represents useful basis to define an extended molecular database of potential relevance both in basic cell biology studies, and in therapeutic options.…”

Section: Cdna Arrays In Melanomamentioning

confidence: 99%

cDNA array technology in melanoma: An overview

Baldi¹,

Santini²,

Luca³

et al. 2003

Journal Cellular Physiology

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Genetic aberrations, mostly resulting in changes in gene expression, are critical events in cancer onset and progression. The advent of the cDNA array technology allows the screening and the efficient measurement of expression of thousands genes simultaneously in a wide spectrum of experimental and clinical models. This genomic scale approach is being currently used to obtain global views of human cancer gene expression and to identify genetic markers that might be important for diagnosis, prognosis, and therapy. This review discusses some recent findings obtained by means of cDNA arrays investigating the human melanoma.

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Section: Cdna Arrays In Melanomamentioning

confidence: 99%

cDNA array technology in melanoma: An overview

Baldi¹,

Santini²,

Luca³

et al. 2003

Journal Cellular Physiology

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“…A study has revealed that IFN induction of IFI 16 in human melanoma cell lines correlates well with inhibition of cell growth (Certa et al, 2001). As the IFI 16 gene is located in the region 1q22, predicted to harbor a senescence gene(s) (Hensler et al, 1994;Karlsson et al, 1996), we investigated the role of IFI 16 in cellular senescence of prostate epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Role of IFI 16, a member of the interferon-inducible p200-protein family, in prostate epithelial cellular senescence

et al. 2003

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Recent studies have implicated interferon signaling in the regulation of cellular senescence. However, the role of specific interferon-inducible proteins in cellular senescence remains to be defined. Here we report that IFI 16, an interferon-inducible transcriptional modulator from the p200-protein family, contributes to cellular senescence of prostate epithelial cells. Normal human prostate epithelial cells (PrEC) in culture expressed detectable levels of IFI 16, and the levels increased more than fourfold when cells approached cellular senescence. Consistent with a role of IFI 16 in cellular senescence, human prostate cancer cell lines either did not express IFI 16 or expressed a variant form, which was primarily detected in the cytoplasm of prostate cancer cells and not in the nucleus. Moreover, overexpression of functional IFI 16 in human prostate cancer cell lines inhibited colony formation. Additionally, ectopic expression of IFI 16 in clonal prostate cancer cell lines was associated with a senescence-like phenotype, production of senescence-associated b-galactosidase (a biochemical marker for cellular senescence), and reduction of S-phase cells in culture. Importantly, upregulation of p21 WAF1 and inhibition of E2F-stimulated transcription accompanied inhibition of cell growth by IFI 16 in prostate cancer cell lines. Collectively, our observations support the idea that increased levels of IFI 16 in PrECs contribute to senescence-associated irreversible cell growth arrest.

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“…To date the gene expression profile induced by IFN-␣2 has been studied predominantly in fibrosarcoma and melanoma cell lines (3,4). Little is known about the transcriptional profiles for other cell lines and nontransformed cells or of donorspecific differences.…”

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confidence: 99%

Cell-type and Donor-specific Transcriptional Responses to Interferon-α

Schlaak

Hilkens

Costa-Pereira

et al. 2002

Journal of Biological Chemistry

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A sensitive, specific, reproducible, robust, and costeffective customized cDNA array system based on established nylon membrane technology has been developed for convenient multisample expression profiling for several hundred genes of choice. The genes represented are easily adjusted (depending on the availability of corresponding cDNAs) and the method is accordingly readily applicable to a wide variety of systems. Here we have focused on the expression profiles for interferon-␣2a, the most widely used interferon for the treatment of viral hepatitis and malignancies, in primary cells (peripheral blood mononuclear cells, T cells, and dendritic cells) and cell lines (Kit255, HT1080, HepG2, and HuH7). Of 150 genes studied, only six were consistently induced in all cell types and donors, whereas 74 genes were induced in at least one cell type. IRF-7 was identified as the only gene exclusively induced in the hematopoietic cells. No gene was exclusively induced in the nonhematopoietic cell lines. In T cells 12, and in dendritic cells, 25 genes were induced in all donors whereas 45 and 42 genes, respectively, were induced in at least one donor. The data suggest that signaling through IFN-␣2 can be substantially modulated to yield significant cell-type and donor-specific qualitative and quantitative differences in gene expression in response to this cytokine under highly standardized conditions.

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High density oligonucleotide array analysis of interferon-α2a sensitivity and transcriptional response in melanoma cells

Cited by 46 publications

References 36 publications

cDNA array technology in melanoma: An overview

cDNA array technology in melanoma: An overview

Role of IFI 16, a member of the interferon-inducible p200-protein family, in prostate epithelial cellular senescence

Cell-type and Donor-specific Transcriptional Responses to Interferon-α

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