High density lipoprotein utilization by dispersed rat luteal cells

Schuler, Linda A.; Langenberg, Karen K.; Gwynne, John T.; Strauss, Jerome F.

doi:10.1016/0005-2760(81)90135-1

Cited by 67 publications

(15 citation statements)

References 43 publications

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“…The experimental conditions reported above are not comparable with those of the study described in [20] in which the rats were not treated with 4-APP and lutropin was absent from the cell incubation medium. We have shown previously that, whereas HDL was able to stimulate progesterone synthesis by luteal cells from rats treated with 4-APP, HDL did not significantly affect progesterone synthesis by luteal cells from rats which had not been treated with 4-APP [3].…”

Section: Resultscontrasting

confidence: 58%

“…It is-necessary to comment upon an apparent discrepancy between the results described above and those reported in [20] which indicated that a phospholipid/cholesterol dispersion was ineffective in stimulating luteal cell progesterone formation. The experimental conditions reported above are not comparable with those of the study described in [20] in which the rats were not treated with 4-APP and lutropin was absent from the cell incubation medium.…”

Section: Resultsmentioning

confidence: 75%

“…It is also well known that luteal cells, incubated in the absence of lutropin, synthesize only about one-tenth of the progesterone which they can make in its presence [l I]. A significant stimulation of progesterone synthesis by lipoproteins or phospholipid/cholesterol dispersions would not, therefore, be expected under the experimental conditions reported in [20].…”

Section: Resultsmentioning

confidence: 89%

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Stimulation of progesterone synthesis, in corpus luteum cells isolated from 4‐amino‐3,4‐d‐pyrazolopyrimidinetreated rats, by cholesterol presented in non‐lipoprotein form

Petrou¹,

Booth²,

Stansfield³

1986

FEBS Letters

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We have previously shown that the administration of (CAPP) to rats reduces progesterone synthesis by cells subsequently isolated from their corpora lutea, and that plasma high density lipoprotein (HDL) can restore this progesterone synthesis to normal. In this paper we demonstrate that a dispersion of phospholipid and cholesterol, but not other sterols, can enhance this 4-APP-disabled progesterone synthesis to the same level as can HDL, thus providing the first direct evidence that cholesterol is the component of HDL upon which rat corpus luteum depends for its ability to synthesize progesterone.

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Section: Resultscontrasting

confidence: 58%

Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 89%

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Stimulation of progesterone synthesis, in corpus luteum cells isolated from 4‐amino‐3,4‐d‐pyrazolopyrimidinetreated rats, by cholesterol presented in non‐lipoprotein form

Petrou¹,

Booth²,

Stansfield³

1986

FEBS Letters

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“…In granulosa cells and luteal cells obtained from a range of non-primate species, both low-density lipoproteins (LDL) and HDL have been shown to stimulate steroidogenesis in granulosa and luteal cells (Savion et al 1981, Schuler et al 1981, Hwang & Menon 1983, Veldhuis et al 1984, Wiltbank et al 1990, Bao et al 1995, Brannian 1997. In human and in non-human primates, the effects of lipoproteins are less clearly defined.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of progesterone production in human granulosa-lutein cells by lipoproteins: evidence for cholesterol-independent actions of high-density lipoproteins

Ragoobir

Abayasekara²,

Bruckdorfer³

et al. 2002

Journal of Endocrinology

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Low-density lipoproteins (LDL) have been consistently reported to stimulate ovarian steroidogenesis, apparently by the provision of cholesterol as a steroidogenic substrate. Recent studies suggest that high-density lipoproteins (HDL) can also deliver cholesterol to support progesterone synthesis in human granulosa-lutein cells. Therefore, this study investigated the contributions of (i) cholesterol delivery, (ii) cyclic AMP and (iii) protein kinase C (PKC) in the steroidogenic responses of human granulosa-lutein cells to HDL and LDL. Over a 24-h treatment incubation, HDL stimulated a larger increase in progesterone output than did LDL at equivalent cholesterol concentrations. Moreover, at equal protein concentrations (100 µg protein/ml), HDL doubled progesterone production by cells co-treated with a maximally effective concentration of 22R-hydroxycholesterol, whereas LDL had no effect on the progesterone response to this membrane-permeable sterol. These observations indicate that the progesterone response to HDL is not solely due to the delivery of cholesterol as a steroidogenic substrate. Over 24 h, the stimulation of progesterone synthesis by HDL was additive with the response to a maximally effective concentration of dibutyryl-cAMP, but was unaffected by the downregulation of PKC activity (by chronic pre-treatment with a tumour-promoting phorbol ester). We have concluded that HDL appears to stimulate progesterone production in human granulosa-lutein cells by a mechanism not solely reliant on cholesterol delivery.

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“…Unlike LDL, which primarily donates cholesterol to cells, HDL is probably involved in reverse cholesterol transport (removal of cellular cholesterol) from some tissues [l], delivery of sterol to others (e.g. steroidogenic tissues [2][3][4]), and possibly both removal from and delivery of cholesterol to the liver. Thus, the processing of HDL is more complex and may involve different mechanisms for different cells.…”

Section: Introduction 2 Experimentalmentioning

confidence: 99%

Partial purification of a high density lipoprotein‐binding protein from rat liver and kidney membranes

Fidge

1986

FEBS Letters

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The existence of a cell receptor which recognises plasma high density lipoprotein (HDL) has been suggested from studies which demonstrate specific binding of HDL3 to cultured cells derived from various tissues in the body. This study provides evidence of a specific HDL‐binding protein in crude plasma membranes prepared from rat kidney and liver. Following separation of solubilised membrane proteins on polyacrylamide gel slabs and ‘Western’ blotting, one major band was identified which bound HDL3, or apo AI or apo AII The protein, which was present in both liver and kidney membranes, was partially purified by repetitive preparative SDS‐polyacrylamide gel electrophoresis and although accompanied by considerable loss of binding activity, could still be detected by the ligand‐blotting procedure used initially to detect its presence in cell membranes.

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High density lipoprotein utilization by dispersed rat luteal cells

Cited by 67 publications

References 43 publications

Stimulation of progesterone synthesis, in corpus luteum cells isolated from 4‐amino‐3,4‐d‐pyrazolopyrimidinetreated rats, by cholesterol presented in non‐lipoprotein form

Stimulation of progesterone synthesis, in corpus luteum cells isolated from 4‐amino‐3,4‐d‐pyrazolopyrimidinetreated rats, by cholesterol presented in non‐lipoprotein form

Stimulation of progesterone production in human granulosa-lutein cells by lipoproteins: evidence for cholesterol-independent actions of high-density lipoproteins

Partial purification of a high density lipoprotein‐binding protein from rat liver and kidney membranes

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