High Density Lipoprotein from Egg Yolk (EYHDL) Improves Dyslipidemia by Mediating Fatty Acids Metabolism in High Fat Diet-induced Obese Mice

Yu, Zhihui; Mao, Changyi; Fu, Xing; Ma, Meihu

doi:10.5851/kosfa.2018.e38

Cited by 19 publications

(19 citation statements)

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“…The biochemical results demonstrated that EY did not induce lipid accumulation in serum and liver in ND diet, and EY could effectively modify the lipid profiles in serum and liver by increasing HDL‐C, decreasing LDL‐C in serum, and thus attenuated hyperlipidemia in HFD. This is consistent with previous reports in our lab showing that EY‐enriched diet could lower serum TG, TC, LDL‐C, hepatic TG, and cholesterol concentrations than those fed normal diet in rats or mice …”

Section: Discussionsupporting

confidence: 93%

Long Term Egg Yolk Consumption Alters Lipid Metabolism and Attenuates Hyperlipidemia in Mice Fed a High‐Fat Diet Based on Lipidomics Analysis

Wang

Ahn

et al. 2019

Euro J Lipid Sci & Tech

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Studies on the association between egg yolk (EY) consumption and obesity are limited and the involved mechanism still needs to be further explored. In this study, the effects of EY consumption under normal and high fat diets on mice based on lipidomics approach is compared. A total of 893 and 925 individual lipid species are determined in serum and liver, respectively. The results reveal that EY can alleviate the hyperlipidemia in HFD‐induced obese mice by increasing serum high‐density lipoprotein cholesterol (HDL‐C) and neutral glycerolipid, hepatic fatty acids (FAs), and the size of adipose tissue cells. OPLS‐DA and S‐plots show that 29 lipid biomarkers in serum, mainly including phosphatidylcholine (PC), triglyceride (TG), and phosphatidylinositol (PI), and 44 lipid biomarkers in liver including TG, lysophosphatidylcholine (lysoPC), and lysophosphatidylethanolamine (lysoPE) are related to obesity caused by HFD. In addition, the regulation of EY on lipid disorders is mainly related to the decreases in serum TG and PC, and hepatic fatty acids (FAs), TG and diacylglycerol (DG), which is closely associated with glycerophospholipid metabolism. These findings suggest that long‐term treatment of EY consumption may not increase NAFLD risk factors in normal diet‐fed mice, further improving the hyperlipidemia in HFD‐induced mice. Practical Applications: This study investigates the overall effects of EY on normal and high‐fat diet by lipidomic analysis. It is concluded that the intake of EY can improve the hyperlipidemia in HFD‐induced obese mice by improving glycerophospholipid metabolism. These results facilitate to comprehensively evaluate the nutrition of EY and help elucidate the favorable effect of eggs. The flow chart of the egg yolk nutritional evaluation based on 100‐d mice experiment. The lipid profiles in serum and liver are further investigated using UPLC‐MS/MS method to elucidate its favorable effect on lipid metabolism.

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Section: Discussionsupporting

confidence: 93%

Long Term Egg Yolk Consumption Alters Lipid Metabolism and Attenuates Hyperlipidemia in Mice Fed a High‐Fat Diet Based on Lipidomics Analysis

Wang

Ahn

et al. 2019

Euro J Lipid Sci & Tech

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“…In the present study, feeding of HFD to male rats increased plasma TC, TG and LDL-c, while it results in a decrease of HDL-c, compared to control rats. There is consistency between our findings and that reported by Yu et al [26] and Barakat and Mahmoud [27] . The observed dyslipidemia was explained by the disorders of lipid metabolism by HFD [26] .…”

Section: Discussionsupporting

confidence: 93%

“…There is consistency between our findings and that reported by Yu et al [26] and Barakat and Mahmoud [27] . The observed dyslipidemia was explained by the disorders of lipid metabolism by HFD [26] . High levels of FAs can result in increased intracellular TG synthesis and formation of lipid droplets in serum and liver [28] .…”

Section: Discussionsupporting

confidence: 93%

The effect of lipid disturbance and vitamin D on the fertility in male albino rats

Hikal

Bayoumi

Ibrahim

et al. 2020

Journal of Recent Advances in Medicine

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Background: Vitamin D has multiple biological effects on male reproductive system. Vitamin D deficiency (VD−) and disturbance of lipid metabolism can induce changes in testicular hormone production and seminal parameters that relate to male infertility. Objective: To investigate the role of vitamin D and lipid metabolism on fertility in male albino rats . Methods:The study was performed on 60 male rats, divided into 6 groups; GI: control group, GII: orlistat group, GIII: orlistat and vitamin D group, GIV: hyperlipidemic group, GV: hyperlipidemic orlistat group and GVI: hyperlipidemic orlistat and vitamin D group. Serum total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-c) and high density lipoprotein cholesterol (HDL-c), 25-hydroxyvitamin D (25(OHD), testosterone (T), inhibin B, follicle-stimulating hormone (FSH) and estradiol (E) levels were determined. Sperm count and viability were also analyzed .Results: Administration of orlistat in GII caused significant alterations of the serum lipid profile, compared to control group. There was interaction between dietary fat and VD− on serum 25 (OHD). A significant VD− was occurred in both GII and GIV, compared to GI. VD− in these groups consequently caused significant decrease in serum T, inhibin B, sperm count and viability and significant increase in FSH and E levels. Administration of orlistat in GV caused significant changes in serum lipid profile, a decrease in E, increase in 25(OHD) and inhibin B, compared to IV. On the other hand, administration of vitamin D with orlistat in GIII and GVI caused significant increase in HDL-c, 25(OHD), T, sperm count and viability and significant decrease in TC, TG, LDLc, FSH and E . Conclusion:Both hyperlipidemia and hypolipidemia were associated with vitamin D deficiency. Moreover, vitamin D has a positive potential effect on male fertility in either hyperlipidemic or hypolipidemic rats .

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“…Histological analysis. The liver tissue was fixed in paraffin and cut into 3-m thick sections (27). The sections were stained with hematoxylin and eosin (H&E), and the stained sections were observed under a microscope.…”

Section: Methodsmentioning

confidence: 99%

Akkermansia muciniphila Prevents Fatty Liver Disease, Decreases Serum Triglycerides, and Maintains Gut Homeostasis

Kim¹,

Lee

Kim

et al. 2020

Appl Environ Microbiol

127

100

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The objective of this study was to elucidate the effect of intestinal Akkermansia muciniphila bacteria on fatty liver disease. Five-week-old C57BL/6N mice were administered either phosphate-buffered saline (PBS; control) or A. muciniphila at 108 to 109 CFU/ml, and were fed either a 45% fat diet (high-fat diet [HFD]) or a 10% fat diet (normal diet [ND]) for 10 weeks. After 10 weeks, the mice were euthanized, and blood and tissue samples, including adipose tissue, cecum, liver, and brain, were immediately collected. Biochemical and histological analyses were conducted, and the expression levels of related factors were compared to determine the antiobesity effects of Akkermansia muciniphila. The gut microbiome was analyzed in fecal samples. Oral administration of A. muciniphila significantly (P < 0.05) lowered serum triglyceride (TG) and alanine aminotransferase (ALT) levels in obese mice. Compared to the non-A. muciniphila-treated group, the expression of SREBP (regulator of TG synthesis in liver tissue) was decreased in the A. muciniphila-treated group. The expression of IL-6 in the liver of obese mice was decreased following the administration of A. muciniphila. Furthermore, alterations in the ratio of Firmicutes to Bacteroidetes and the decrease in bacterial diversity caused by the HFD were restored upon the administration of A. muciniphila. These results indicate that A. muciniphila prevents fatty liver disease in obese mice by regulating TG synthesis in the liver and maintaining gut homeostasis. IMPORTANCE This study investigated the effect of Akkermansia muciniphila on fatty liver disease. Although some research about the effects of A. muciniphila on host health has been published, study of the relationship between A. muciniphila administration and fatty liver, as well as changes in the gut microbiota, has not been conducted. In this study, we demonstrated that A. muciniphila prevented fatty liver disease by regulation of the expression of genes that regulate fat synthesis and inflammation in the liver.

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High Density Lipoprotein from Egg Yolk (EYHDL) Improves Dyslipidemia by Mediating Fatty Acids Metabolism in High Fat Diet-induced Obese Mice

Cited by 19 publications

References 50 publications

Long Term Egg Yolk Consumption Alters Lipid Metabolism and Attenuates Hyperlipidemia in Mice Fed a High‐Fat Diet Based on Lipidomics Analysis

Long Term Egg Yolk Consumption Alters Lipid Metabolism and Attenuates Hyperlipidemia in Mice Fed a High‐Fat Diet Based on Lipidomics Analysis

The effect of lipid disturbance and vitamin D on the fertility in male albino rats

Akkermansia muciniphila Prevents Fatty Liver Disease, Decreases Serum Triglycerides, and Maintains Gut Homeostasis

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