High degree of organization of bacteriochlorophyll c in chlorosome-like aggregates spontaneously assembled in aqueous solution

Hirota, Masamitsu; Moriyama, Takeshi; Shimada, Keizo; Miller, Megan; Olson, John M.; Matsuura, Kiyotaka

doi:10.1016/0005-2728(92)90038-4

Cited by 85 publications

(36 citation statements)

References 16 publications

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“…limicola are available [21,22]. Here we report 3D LD component spectra (3D polarized light absorption spectra) of chlorosomes from some Chlorobium species containing BChl-c, d or e in a compressed polyacrylamide gel, providing useful information for the understanding of supramolecular structures of chlorosomal aggregates and also for characterization of their electronic absorption bands.…”

Section: Introductionmentioning

confidence: 98%

Linearly polarized light absorption spectra of chlorosomes, light-harvesting antennas of photosynthetic green sulfur bacteria

Tamiaki

Tateishi

Nakabayashi

et al. 2010

Chemical Physics Letters

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Section: Introductionmentioning

confidence: 98%

Linearly polarized light absorption spectra of chlorosomes, light-harvesting antennas of photosynthetic green sulfur bacteria

Tamiaki

Tateishi

Nakabayashi

et al. 2010

Chemical Physics Letters

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“…It is known that BChl c forms (higher state) aggregates in aqueous buffer in the presence of lipids, e.g. MGDG (1,2,22,26). We have observed similar aggregates also when using the X‐BChlide derivatives.…”

Section: Resultsmentioning

confidence: 99%

“…Photochemistry and Photobiology, 2008, 84: 1187-1194 nonpolar solvents or in aqueous buffers with the addition of lipids, detergents or carotenoids (20)(21)(22)(23). Self-aggregation was also reported for synthetic chlorins with the hydroxy and keto groups located along the Q y axis of the chlorin derivative and with coordinated central metal ions (24,25); however, it is also possible to use other recognition groups for the formation of synthetic BChl aggregate mimics (26).…”

Section: Introductionmentioning

confidence: 99%

The Length of Esterifying Alcohol Affects the Aggregation Properties of Chlorosomal Bacteriochlorophylls

Zupčanová¹,

Arellano²,

Bína³

et al. 2008

Photochem & Photobiology

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Chlorosomes, the main light-harvesting complexes of green photosynthetic bacteria, contain bacteriochlorophyll (BChl) molecules in the form of self-assembling aggregates. To study the role of esterifying alcohols in BChl aggregation we have prepared a series of bacteriochlorophyllide c (BChlide c) derivatives differing in the length of the esterifying alcohol (C(1), C(4), C(8) and C(12)). Their aggregation behavior was studied both in polar (aqueous buffer) and nonpolar (hexane) environments and the esterifying alcohols were found to play an essential role. In aqueous buffer, hydrophobic interactions among esterifying alcohols drive BChlide c derivatives with longer chains into the formation of dimers, while this interaction is weak for BChlides with shorter esterifying alcohols and they remain mainly as monomers. All studied BChlide c derivatives form aggregates in hexane, but the process slows down with longer esterifying alcohols due to competing hydrophobic interactions with hexane molecules. In addition, the effect of the length of the solvent molecules (n-alkanes) was explored for BChl c aggregation. With an increasing length of n-alkane molecules, the hydrophobic interaction with the farnesyl chain becomes stronger, leading to a slower aggregation rate. The results show that the hydrophobic interaction is the driving force for the aggregation in an aqueous environment, while in nonpolar solvents it is the hydrophilic interaction.

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“…The hydrophobic interaction between the BChl c esterifying alcohol and the MGDG esterifying acid tails is expected to be the driving force for the BChl c aggregation also in this case. It is well documented that artificial MGDG aggregates of BChl c can be formed with no need of proteins or Car in aqueous buffer, reaching absorption and circular dichroism spectra very similar to those of chlorosomes ( 11,30,38 ). It is assumed that in artificial MGDG aggregates, a monolayer of MGDG surrounds the BChl c aggregates as in chlorosomes, forming a hydrophobic interior, which favors the intermolecular interaction among BChl.…”

Section: Discussionmentioning

confidence: 99%

Effect of Carotenoids and Monogalactosyl Diglyceride on Bacteriochlorophyll c Aggregates in Aqueous Buffer: Implications for the Self‐assembly of Chlorosomes^¶

Klinger

Arellano

Vácha

et al. 2004

Photochem & Photobiology

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Aggregation of bacteriochlorophyll (BChl) c from chlorosomes, the main light‐harvesting complex of green bacteria, has been studied in aqueous buffer. Unlike other chlorophyll‐like molecules, BChl c is rather soluble in aqueous buffer, forming dimers. When BChl c is mixed with carotenoids (Car), the BChl c Qy transition is further redshifted, in respect to that of monomers and dimers. The results suggest that Car are incorporated in the aggregates and induce further aggregation of BChl c. The redshift of the BChl c Qy band is proportional to the Car concentration. In contrast, the mixture of bacteriochlorophyllide (BChlide) c, which lacks the nonpolar esterifying alcohol, does not form aggregates with Car in aqueous buffer or nonpolar solvents. Instead, the position of the BChlide c Qy transition remains unshifted in respect to that of the monomeric molecule, and Car precipitates with the course of time in aqueous buffer. Similar effects on both BChl c and BChlide c are also observed when monogalactosyl diglyceride (MGDG), which forms the monolayer envelope of chlorosomes, is used instead of (or together with) Car. The results show that the hydrophobic interactions of the BChl c esterifying alcohols with themselves and the nonpolar carbon skeleton of Car, or the fatty acid tails of MGDG, are essential driving forces for BChl aggregation in chlorosomes.

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High degree of organization of bacteriochlorophyll c in chlorosome-like aggregates spontaneously assembled in aqueous solution

Cited by 85 publications

References 16 publications

Linearly polarized light absorption spectra of chlorosomes, light-harvesting antennas of photosynthetic green sulfur bacteria

Linearly polarized light absorption spectra of chlorosomes, light-harvesting antennas of photosynthetic green sulfur bacteria

The Length of Esterifying Alcohol Affects the Aggregation Properties of Chlorosomal Bacteriochlorophylls

Effect of Carotenoids and Monogalactosyl Diglyceride on Bacteriochlorophyll c Aggregates in Aqueous Buffer: Implications for the Self‐assembly of Chlorosomes^¶

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High degree of organization of bacteriochlorophyll c in chlorosome-like aggregates spontaneously assembled in aqueous solution

Cited by 85 publications

References 16 publications

Linearly polarized light absorption spectra of chlorosomes, light-harvesting antennas of photosynthetic green sulfur bacteria

Linearly polarized light absorption spectra of chlorosomes, light-harvesting antennas of photosynthetic green sulfur bacteria

The Length of Esterifying Alcohol Affects the Aggregation Properties of Chlorosomal Bacteriochlorophylls

Effect of Carotenoids and Monogalactosyl Diglyceride on Bacteriochlorophyll c Aggregates in Aqueous Buffer: Implications for the Self‐assembly of Chlorosomes¶

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Effect of Carotenoids and Monogalactosyl Diglyceride on Bacteriochlorophyll c Aggregates in Aqueous Buffer: Implications for the Self‐assembly of Chlorosomes^¶