High-cooling-efficiency cryogenic quadrupole ion trap and UV-UV hole burning spectroscopy of protonated tyrosine

Ishiuchi, Shun‐ichi; Wako, Hiromichi; Kato, Daichi; Fujii, Masaaki

doi:10.1016/j.jms.2016.10.011

Cited by 67 publications

(76 citation statements)

References 30 publications

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“…Figure shows a schematic diagram of the ESI source and cryogenic ion trap used for this study. Additional details are provided elsewhere . Ac‐Tyr‐NHMe (GYG; 2×10 −4 M purchased from BACHEM) and MCl (M=Li, Rb, Cs) or (CH 3 COO) 2 M (M=Mg, Ca, Sr, Ba) (1×10 −4 M) were dissolved in pure methanol.…”

Section: Methodsmentioning

confidence: 99%

“…However, as the burn laser (which irradiates the trapped ions before the probe laser) is scanned in frequency, it can dissociate any conformer that has a resonant vibrational transition. These photofragments are eliminated from the ion trap by applying an auxiliary radio frequency (RF) pulse between burn and probe lasers (tickling technique) . When the burn laser scans through any vibrational transition of the probed conformer, the IRPD signal generated by the probe laser will be reduced since the burn laser has already depleted a fraction of that conformer.…”

Section: Methodsmentioning

confidence: 99%

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Alkali and Alkaline Earth Metal Ions Complexes with a Partial Peptide of the Selectivity Filter in K⁺ Channels Studied by a Cold Ion Trap Infrared Spectroscopy

et al. 2020

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The infrared (IR) spectra of alkali and alkaline earth metal ion complexes with the Ac‐Tyr‐NHMe (GYG) peptide have been measured by laser photodissociation in a cold ion trap coupled with an electrospray mass spectrometer. The GYG peptide corresponds to a portion of the ion selectivity filter in the KcsA K+ channel that allows K+ to pass, but blocks Na+ even though it has a smaller ionic radius than K+. This current study extends a previous investigation on Na+ and K+ to the entire set of alkali metaI ions and alkaline earth dications. IR‐IR hole‐burning (IR2 dip) spectroscopy has established the coexistence of several conformers of the GYG‐metal ion complexes. The structures of the conformers were assigned by comparison between the isomer‐selected IR spectra and theoretical IR spectra obtained from quantum chemical calculations. It was found that the structure of the dominant conformer correlates with the ability of the ion to permeate through the K+ channel.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Alkali and Alkaline Earth Metal Ions Complexes with a Partial Peptide of the Selectivity Filter in K⁺ Channels Studied by a Cold Ion Trap Infrared Spectroscopy

et al. 2020

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“…The development of UV/UV hole burning spectroscopy in cold traps has added a further tool that can be used to differentiate tautomers. [17][18][19] In the present paper, we have studied protonated 1-and 2-aminonaphthalene with the aim of answering the following questions: 3 1) How does the proton affinity of polycyclic aromatic hydrocarbons (PAHs) change when a -NH 2 substituent is added? In particular, we will compare aminonaphthalene and naphthalene 20 .…”

Section: Introductionmentioning

confidence: 99%

Tautomerism and electronic spectroscopy of protonated 1- and 2-aminonaphthalene

Noble

Broquier

Grégoire

et al. 2018

Phys. Chem. Chem. Phys.

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Experimental and theoretical investigations of the excited states of protonated 1- and 2-aminonaphthalene are presented. The electronic spectra are obtained by laser induced photofragmentation of the ions captured in a cold ion trap. Using ab initio calculations, the electronic spectra can be assigned to different tautomers which have the proton on the amino group or on the naphthalene moiety. It is shown that the tautomer distribution can be varied by changing the electrospray source conditions, favoring either the most stable form in solution (amino protonation) or that in the gas phase (aromatic ring protonation). Calculations for larger amino-polyaromatics predict that these systems should behave as "proton sponges" i.e. have a proton affinity larger than 11 eV.

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“…Designs of cryogenic ion traps are another area that requires further developments in order to make the technique compatible with the high sensitivity requirements of biological samples. Cryogenic trap designs thus far have emphasized the capabilities of these traps to cool ions down to very low temperatures . Tagged ions are then generally moved to other mass analyzers, such as a time‐of‐flight drift tube for mass‐selective manipulation.…”

Section: Introductionmentioning

confidence: 99%

Infrared ion spectroscopy inside a mass‐selective cryogenic 2D linear ion trap

et al. 2017

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We demonstrate operation of the first cryogenic 2D linear ion trap (LIT) with mass-selective capabilities. This trap presents a number of advantages for infrared ion “action” spectroscopy studies, particularly those employing the “tagging/messenger” spectroscopy approach. The high trapping efficiencies, trapping capacities, and low detection limits make 2D LITs a highly suitable choice for low-concentration analytes from scarce biological samples. In our trap, ions can be cooled down to cryogenic temperatures to achieve higher-resolution infrared spectra, and individual ions can be mass selected prior to irradiation for a background-free photodissociation scheme. Conveniently, multiple tagged analyte ions can be mass isolated and efficiently irradiated in the same experiment, allowing their infrared spectra to be recorded in parallel. This multiplexed approach is critical in terms of increasing the duty cycle of infrared ion spectroscopy, which is currently a key weakness of the technique. The compact design of this instrument, coupled with powerful mass selection capabilities, set the stage for making cryogenic infrared ion spectroscopy viable as a bioanalytical tool in small molecule identification.

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High-cooling-efficiency cryogenic quadrupole ion trap and UV-UV hole burning spectroscopy of protonated tyrosine

Cited by 67 publications

References 30 publications

Alkali and Alkaline Earth Metal Ions Complexes with a Partial Peptide of the Selectivity Filter in K⁺ Channels Studied by a Cold Ion Trap Infrared Spectroscopy

Alkali and Alkaline Earth Metal Ions Complexes with a Partial Peptide of the Selectivity Filter in K⁺ Channels Studied by a Cold Ion Trap Infrared Spectroscopy

Tautomerism and electronic spectroscopy of protonated 1- and 2-aminonaphthalene

Infrared ion spectroscopy inside a mass‐selective cryogenic 2D linear ion trap

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High-cooling-efficiency cryogenic quadrupole ion trap and UV-UV hole burning spectroscopy of protonated tyrosine

Cited by 67 publications

References 30 publications

Alkali and Alkaline Earth Metal Ions Complexes with a Partial Peptide of the Selectivity Filter in K+ Channels Studied by a Cold Ion Trap Infrared Spectroscopy

Alkali and Alkaline Earth Metal Ions Complexes with a Partial Peptide of the Selectivity Filter in K+ Channels Studied by a Cold Ion Trap Infrared Spectroscopy

Tautomerism and electronic spectroscopy of protonated 1- and 2-aminonaphthalene

Infrared ion spectroscopy inside a mass‐selective cryogenic 2D linear ion trap

Contact Info

Product

Resources

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Alkali and Alkaline Earth Metal Ions Complexes with a Partial Peptide of the Selectivity Filter in K⁺ Channels Studied by a Cold Ion Trap Infrared Spectroscopy

Alkali and Alkaline Earth Metal Ions Complexes with a Partial Peptide of the Selectivity Filter in K⁺ Channels Studied by a Cold Ion Trap Infrared Spectroscopy