High Contrast Surface Enhanced Fluorescence of Carbon Dot Labeled Bacteria Cells on Aluminum Foil

Bukasov, Rostislav; Kunushpayeva, Zhanar; Rapikov, Alisher; Zhunussova, Saida; Sultangaziyev, Alisher; Filchakova, Olena

doi:10.1007/s10895-020-02610-2

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…Recent publications have reported the application of Al alloy and Al foil as substrates for SERS with visible excitation [ 25 , 26 , 27 ]. Several other reports also exist on the efficient application of Al foil, sometimes performing on par with gold film, as a substrate for surface-enhanced spectroscopies, including surface-enhanced fluorescence [ 28 , 29 , 30 ] and SERS [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Aluminum Foil vs. Gold Film: Cost-Effective Substrate in Sandwich SERS Immunoassays of Biomarkers Reveals Potential for Selectivity Improvement

Bukasov

Sultangaziyev

Kunushpayeva

et al. 2023

IJMS

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The first application of aluminum foil (Al F) as a low-cost/high-availability substrate for sandwich immunoassay using surface-enhanced Raman spectroscopy (SERS) is reported. Untreated and unmodified Al F and gold film are used as substrates for sandwich SERS immunoassay to detect tuberculosis biomarker MPT64 and human immunoglobulin (hIgG) in less than 24 h. The limits of detection (LODs) for tuberculosis (TB) biomarker MPT64 on Al foil, obtained with commercial antibodies, are about 1.8–1.9 ng/mL, which is comparable to the best LOD (2.1 ng/mL) reported in the literature for sandwich ELISA, made with fresh in-house antibodies. Not only is Al foil competitive with traditional SERS substrate gold for the sandwich SERS immunoassay in terms of LOD, which is in the range 18–30 pM or less than 1 pmol of human IgG, but it also has a large cost/availability advantage over gold film. Moreover, human IgG assays on Al foil and Si showed better selectivity (by about 30–70% on Al foil and at least eightfold on Si) and a nonspecific response to rat or rabbit IgG, in comparison to the selectivity in assays using gold film.

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Section: Introductionmentioning

confidence: 99%

Aluminum Foil vs. Gold Film: Cost-Effective Substrate in Sandwich SERS Immunoassays of Biomarkers Reveals Potential for Selectivity Improvement

Bukasov

Sultangaziyev

Kunushpayeva

et al. 2023

IJMS

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“…Al foil is a promising non-conventional substrates for SERS, which is not only easily and readily available in most places of the world, but it may also have better resistance to contamination and non-specific protein binding in bio-sensing applications [ 23 ]. Al foil has been reported as an SERS and SEF substrate in a number of sensing and bio-sensing applications [ 24 , 25 , 26 , 27 ]. Silicon has a lower cost and higher stability/resistance to contamination/oxidation in comparison to silver and gold substrates, which are prone to contamination/non-specific binding from S-containing compounds, including proteins, especially due to Au-S and Ag-S dative bonding [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Silicon has a lower cost and higher stability/resistance to contamination/oxidation in comparison to silver and gold substrates, which are prone to contamination/non-specific binding from S-containing compounds, including proteins, especially due to Au-S and Ag-S dative bonding [ 23 ]. Therefore, silicon has attracted increasing attention as a substrate for surface-enhanced spectroscopy for sensing and bio-sensing applications [ 27 , 28 ]. Bare silicon is very seldom used as a substrate for surface-enhanced Raman spectroscopy (SERS) [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

SERS for Detection of Proteinuria: A Comparison of Gold, Silver, Al Tape, and Silicon Substrates for Identification of Elevated Protein Concentration in Urine

Aitekenov

Sultangaziyev

Boranova

et al. 2023

Sensors

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Excessive protein excretion in human urine is an early and sensitive marker of diabetic nephropathy and primary and secondary renal disease. Kidney problems, particularly chronic kidney disease, remain among the few growing causes of mortality in the world. Therefore, it is important to develop an efficient, expressive, and low-cost method for protein determination. Surface enhanced Raman spectroscopy (SERS) methods are potential candidates to achieve these criteria. In this paper, a SERS method was developed to distinguish patients with proteinuria from the healthy group. Commercial gold nanoparticles (AuNPs) with diameters of 60 nm and 100 nm, and silver nanoparticles (AgNPs) with a diameter of 100 nm were tested on the surface of four different substrates including silver and gold films, silicon, and aluminum tape. SERS spectra were acquired from 111 unique human urine samples prepared and measured for each of the seven different nanoparticle plus substrate combinations. Data analysis by the PCA-LDA algorithm and the ROC curves gave results for the diagnostic figures of merits. The best sensitivity, specificity, accuracy, and AUC were 0.91, 0.84, 0.88, and 0.94 for the set with 100 nm Au NPs on the silver substrate, respectively. Among the three metal substrates, the substrate with AuNPs and Al tape performed slightly worse than the other three substrates, and 100 nm gold nanoparticles on average produced better results than 60 nm gold nanoparticles. The 60 nm diameter AuNPs and silicon, which is about one order of magnitude more cost-effective than AuNPs and gold film, showed a relative performance close to the performance of 60 nm AuNPs and Au film (average AUC 0.88 (Si) vs. 0.89 (Au)). This is likely the first reported application of unmodified silicon in SERS substrates applied for direct detection of proteins in any biofluid, particularly in urine. These results position silicon and AuNPs@Si in particular as a perspective SERS substrate for direct urine analysis, including clinical diagnostics of proteinuria.

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“…3 Metal nanostructures and metal colloidal nanoparticles (NPs) are effective for increasing uorophores' uorescence because they can interact with proximal uorophores at optimal distances of 5-90 nm to increase the quantum yield; this phenomenon is known as surface-enhanced uorescence or metal-enhanced uorescence (MEF). 4,5 Under photon excitation, noble-metal NPs generate plasma to amplify the excitation of uorophores close to their surface, enhancing the uorescence. 6 Gontero et al deposited Au@SiO 2 -rhodamine B NPs on Escherichia coli bacteria for bioimaging over their surface and reported an optimal enhancement factor of 9.5 for 10 nm silica spacers.…”

Section: Introductionmentioning

confidence: 99%

Metal-enhanced fluorescence through conventional Ag-polyethylene glycol nanoparticles for cellular imaging

Chen,

Wu,

et al. 2023

RSC Adv.

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High Contrast Surface Enhanced Fluorescence of Carbon Dot Labeled Bacteria Cells on Aluminum Foil

Cited by 7 publications

References 34 publications

Aluminum Foil vs. Gold Film: Cost-Effective Substrate in Sandwich SERS Immunoassays of Biomarkers Reveals Potential for Selectivity Improvement

Aluminum Foil vs. Gold Film: Cost-Effective Substrate in Sandwich SERS Immunoassays of Biomarkers Reveals Potential for Selectivity Improvement

SERS for Detection of Proteinuria: A Comparison of Gold, Silver, Al Tape, and Silicon Substrates for Identification of Elevated Protein Concentration in Urine

Metal-enhanced fluorescence through conventional Ag-polyethylene glycol nanoparticles for cellular imaging

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