High‐Contrast Reversible Fluorescence Photoswitching of Dye‐Crosslinked Dendritic Nanoclusters in Living Vertebrates

Kim, Yoonkyung; Jung, Hye‐youn; Choe, Yun H.; Lee, Chaewoon; Ko, Sung-Kyun; Koun, Soonil; Choi, Youngin; Chung, Bong Hyun; Park, Byoung Chul; Huh, Tae‐Lin; Shin, Injae; Kim, Eunkyoung

doi:10.1002/ange.201107086

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…Another increasingly common class of organic photochromic dyes includes the diarylethene-based photoswitchable compounds, which, like the spiro-based materials, undergo a structural open-closed photochromic transition [152,153,[165][166][167].…”

Section: Photochromic Dyesmentioning

confidence: 99%

“…Diarylethene materials have been used to create photoswitchable dendrimers, where the diarylethene acts as a FRET acceptor that quenches the attached Cy3 donor emission via FRET, when switched off in the closed form [167]. Photochromic FRET imaging using this dendrimer was demonstrated within both HeLa cells and zebra fish, with the potential for use in detailed imaging of cellular processes within cells or organisms.…”

Section: Photochromic Dyesmentioning

confidence: 99%

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Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

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The intrinsic sensitivity (r 6 dependence) of F€ orster (or fluorescence) resonance energy transfer (FRET) to nanoscale changes in the donor/acceptor separation distance has made FRET an invaluable biophysical tool in a variety of applications, ranging from studying the structure and conformation of proteins and nucleic acids to examining biomolecular interactions, including its use in in vitro and in vivo bioassays [1][2][3][4][5][6][7]. While the myriad of FRET configurations and techniques currently in use are covered throughout this book, here we focus primarily on the materials utilized as donor or acceptor probes in FRET rather than the process itself [3,5,8,9]. Our 2006 review paper on this topic serves as the foundation for this updated chapter [3]. The materials were divided into three main categories: organic materials that include "traditional" dye fluorophores, dark quenchers, polymers, and carbon nanomaterials (NMs); inorganic materials such as metal chelates, metal, and semiconductor nanocrystals; and fluorophores of biological origin such as fluorescent proteins (FPs), amino acids, and fluorescence generated from enzymatic bioluminescence (BL) and chemiluminescence (CL). These materials may function as FRET donors and/or acceptors, depending upon experimental design. Many of the new materials developed and/or new donor-acceptor probe combinations used address some of the inherent complications of more traditional FRET materials, including photobleaching, spectral cross talk, and direct excitation of the acceptor species, and examples of these will be highlighted and discussed throughout the chapter. Since the vast majority of FRET applications are biological in nature, they routinely involve some type of biomolecule labeling strategy, which ultimately plays a significant and fundamental role in the success and interpretation of the resulting FRET. Therefore, we begin the chapter with a brief discussion of the bioconjugation techniques commonly utilized for FRET and points to consider, followed by sections highlighting the current and emerging materials that are used, or have the potential to be used, in FRET applications.

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Section: Photochromic Dyesmentioning

confidence: 99%

Section: Photochromic Dyesmentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

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“…[1] A few types of electrofluorochromic (EFC) materials were reported in the following few years, [2] but the utilization of the EFC functionalities of the materials in various fields was sparsely explored. These functional materials have only recently gained more attention owing to their potential applications in areas such as fluorescence imaging, [3] optical memories, [4] and sensors. [5] Commonly studied EFC materials include molecular dyads, conjugated polymers (CPs), and intrinsically switchable fluorophores.…”

Section: Introductionmentioning

confidence: 99%

Electrofluorochromic Detection of Cyanide Anions Using a Nanoporous Polymer Electrode and the Detection Mechanism

Ding¹,

Lin²,

Zhou³

et al. 2014

Chemistry A European J

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An electrofluorochromic (EFC) conjugated copolymer (PEFC) containing carbazole and benzothiadiazole (BTD) moieties is synthesized through Suzuki coupling followed by electrochemical polymerization, resulting in a nanoporous EFC polymer electrode. The electrode exhibits high sensitivity and selectivity in the EFC detection of cyanide anions (CN(-)) in largely aqueous electrolyte (67 vol % water) because electrochemical oxidation of PEFC leads to significant fluorescence quenching, and the presence of different concentrations (1 to 100 μM) of CN(-) in the electrolyte can weaken the oxidative quenching to substantially different extents. Although PEFC is hydrophobic in the neutral state, it is converted to radical cation/dication states upon oxidation, rendering the PEFC some hydrophilicity. Moreover, its nanoporous morphology provides a large surface area and short diffusion distance, facilitating the movement of CN(-) in the electrolyte into the PEFC film to interact with receptors. Density functional theory calculations show that the noncovalent interaction between electron-deficient BTD and nucleophilic CN(-) is energy favorable in the oxidized states in both aqueous and organic media, suggesting that the specific π(-)-π(+) interaction plays the main role in the CN(-) detection.

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Giant Amplification of Photoswitching by a Few Photons in Fluorescent Photochromic Organic Nanoparticles

Fukaminato

Placial

et al. 2016

Angewandte Chemie

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Controlling or switching the optical signal from a large collection of molecules with the minimum of photons represents an extremely attractive concept. Promising fundamental and practical applications may be derived from such a photon‐saving principle. With this aim in mind, we have prepared fluorescent photochromic organic nanoparticles (NPs), showing bright red emission, complete ON–OFF contrast with full reversibility, and excellent fatigue resistance. Most interestingly, upon successive UV and visible light irradiation, the NPs exhibit a complete fluorescence quenching and recovery at very low photochromic conversion levels (<5 %), leading to the fluorescence photoswitching of 420±20 molecules for only one converted photochromic molecule. This “giant amplification of fluorescence photoswitching” originates from efficient intermolecular energy‐transfer processes within the NPs.

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High‐Contrast Reversible Fluorescence Photoswitching of Dye‐Crosslinked Dendritic Nanoclusters in Living Vertebrates

Cited by 6 publications

References 37 publications

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Electrofluorochromic Detection of Cyanide Anions Using a Nanoporous Polymer Electrode and the Detection Mechanism

Giant Amplification of Photoswitching by a Few Photons in Fluorescent Photochromic Organic Nanoparticles

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