High‐Contrast Fluorescence Diagnosis of Cancer Cells/Tissues Based on β‐Lapachone‐Triggered ROS Amplification Specific in Cancer Cells

Abstract

Discrimination of cancer cells/tissues from normal ones is of critical importance for early diagnosis and treatment of cancers.Herein, we present anew strategy for high-contrast fluorescence diagnosis of cancer cells/tissues based on b-Lapachone (b-Lap,a na nticancer agent) triggered ROS (reactive oxygen species) amplification specific in cancer cells/tissues.W ith the strategy,awide range of cancer cells/ tissues,i ncluding surgical tissue specimens harvested from patients,w ere distinguished from normal ones by using ac ombination of b-Lap and aS i-rhodamine-based NIR fluorescent ROS probe PSiR3 developed in this work with average tumor-to-normal (T/N) ratios up to 15 in cell level and 24 in tissue level, far exceeding the clinically acceptable threshold of 2.0. Whatsm ore,t he strategy allowed the fluorescence discrimination of tumor tissues from inflammatory ones based on whether am arked fluorescence enhancement could be induced when treated with PSiR3 and b-Lap/ PSiR3 combination, respectively.

A lipid droplet-targetable and biothiol-sensitive fluorescent probe for the diagnosis of cancer cells/tissues

“…Visualization of cancer cells/tissues using BTDA-RSS Mounting evidence suggests that cancer cells are usually characterized by some intracellular microenvironment mutations (e.g., ROS, GSH, pH, polarity and viscosity), 6,41,42,48,49 prompting us to explore whether BTDA-RSS can distinguish cancer cells from normal cells through imaging the biothiol level in LDs. Accordingly, different types of cancer cells (HeLa, 4T1 and A549) and normal cells (MPC5, PC12 and TM3) were tested after treating with BTDA-RSS.…”

An Edaravone-Guided Design of a Rhodamine-Based Turn-on Fluorescent Probe for Detecting Hydroxyl Radicals in Living Systems

“…As shown in Figure 5, when these cells were incubated with RH-EDA in parallel for 30 min, the HL-7702 cells (normal cells) produced minimal fluorescence (Figure 5b), while an obvious intracellular fluorescence was observed for HepG2 cells (cancer cells) under the same conditions (Figure 5e), indicative of a relatively higher basal level of •OH in cancer cells than normal cells. 52,53 Furthermore, the above RH-EDA-loaded cells were incubated with β-Lap for 2 h. In this case, the intracellular fluorescence of HepG2 cells showed an ∼13.8-fold increase when compared with that of HL-7702 cells (Figure 5c,f); a much higher fluorescence contrast ratio was obtained as compared to the β-Lap-free conditions, indicating the key role of β-Lap for achieving a high contrast ratio between normal and cancer cells.…”

Silicon functionalization expands the repertoire of Si-rhodamine fluorescent probes

“…16,17 Despite this intense interest, and the extension of the bridge-substitution strategy to other elements, 3,18–22 virtually all Si-dyes reported thus far have been confined to dimethylsilyl substitution. 19,23,24 Belying the nearly exclusive reliance on dimethylsilyl bridging groups amongst long-wavelength Si-dyes, a number of silyl substitutions are known within silole optical materials. 1,25–27…”