High continuing pregnancy rates after in vitro fertilization-embryo transfer using medium supplemented with a plasma protein fraction  containing α- and β-globulins

Pool, Thomas B.; Martin, Joseph E.

doi:10.1016/s0015-0282(16)56651-5

Cited by 65 publications

(27 citation statements)

References 19 publications

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“…In the 1980s, human serum was considered as the common albumin source; since then, the whole serum was replaced by albumin sources. Recently, human serum albumin (HSA) has been widely used as an embryo culture supplement to improve clinical outcome (Adler, Reing, Bedford, Alikani, & Cohen, ; Coello, Campos, Remohí, Meseguer, & Cobo, ; Menezo, Testart, & Perrone, ; Pool & Martin, ).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Mohammadzadeh

Safdarian

Amidi

et al. 2017

Reprod Domestic Animals

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The aim of this study was to evaluate the effects of the two types of media, namely minimum essential medium (αMEM) and Ham'sF10, supplemented with foetal bovine serum (FBS) or bovine serum albumin (BSA) in vitrification/warming solution on the quality and viability of sheep ovarian follicles. Vitrification method was applied for cryopreservation of sheep ovarian cortex using Ham'sF10 and αMEM supplemented with either BSA or FBS. There were five groups: Fresh, Ham'sF10+ BSA, Ham'sF10+ FBS, αMEM + BSA and αMEM + FBS. Samples were cultured for two weeks after warming. Viability and morphology of follicles and DNA fragmentation in follicles and in tissue stroma cells were analysed before vitrification/warming and following one and two weeks of culture. The Ham'sF10+ FBS and Ham'sF10+ BSA groups showed a significant decrease in follicular viability after one week of culture (p < .05 vs. Fresh). Following two weeks of culture, all groups revealed a considerable fall in the number of viable follicles (p < .05 vs. Fresh). There was an increase in DNA fragmentation of connective tissue cells but not in the follicles (p < .05). Our results showed the better application of αMEM supplemented with BSA as a vitrification solution in improvement of cryopreservation effects and maintenance of follicular survival.

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Section: Introductionmentioning

confidence: 99%

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Mohammadzadeh

Safdarian

Amidi

et al. 2017

Reprod Domestic Animals

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“…However, SSS contains 6% proteins, (only 5% of which is pharmaceutical grade human serum albumin and ∼1% human globulins heat-treated at 60 • C). The presence of globulin with hSA has been shown to enhance the rate of embryo development and pregnancy rates beyond the levels achieved for hSA alone (14,15).…”

Section: Discussionmentioning

confidence: 98%

Prospective Randomized Comparison of Two Embryo Culture Systems: P1 Medium by Irvine Scientific and the Cook IVF Medium

Ben‐Yosef

Amit

Azem

et al. 2004

J Assist Reprod Genet

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Purpose : To compare the efficacy of two commercially available in vitro fertilization (IVF) and embryo culture media systems: the glucose-free P1 Medium supplemented with 20% synthetic serum substitute (SSS) (Irvine Scientific), and the Cook IVF Medium (Cook, Australia). Methods : A prospective randomized study. Medical center-based IVF Unit affiliated to the Faculty of Medicine of Tel Aviv University. IVF patients were randomly assigned to either P1 Medium supplemented with 20% SSS (182 patients, 196 cycles) or Cook Medium (167 patients, 179 cycles). Results : Fertilization rates were similar with both media (52.3 ± 26.1 and 53.8 ± 27.6, respectively). Likewise, no difference was found in morphological characteristics and grading of cultured embryos. However, a significantly higher proportion of the embryos incubated in the P1 Medium reached the four-cell stage on day 2 or the 6-cell stage on day 3 postfertilization, compared to those incubated in Cook Medium (54.3% vs. 41.9%, p < 0.0001). Clinical pregnancy and delivery rates were improved when oocytes and embryos were cultured in P1 Medium. Finally, Implantation rate was significantly higher in the P1 Medium Group (9.9% vs. 6%, respectively). Conclusions : Our results suggest that the P1 Medium may be associated with a higher embryo cleavage rate and improved implantation rates compared to the Cook IVF Medium.

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“…The addition of a macromolecule such as serum albumin prevents gamete and embryos from becoming ''sticky'' whereby their surface charges make them stick to both glass and plastic. These range from therapeutic albumin solutions (202,204,206) to globulin enriched albumin solutions such as Plasmanate (207), Plasmatein (208) and Synthetic Serum Substitute (SSS) (209)(210)(211). Furthermore, albumin can negate the effects of toxins (201).…”

Section: Protein/macromoleculesmentioning

confidence: 99%

“…Macromolecules, therefore, facilitate gamete and embryo manipulation. Glycoproteins, which possess numerous hydroxyl groups, may confer benefit to the embryo by altering the solvent properties of the medium, making it more akin to the tubal environment (208,212). Both human serum albumin (HSA) (46,(202)(203)(204) and bovine serum albumin (BSA) (205) have been used successfully in the culture of human embryos.…”

Section: Protein/macromoleculesmentioning

confidence: 99%

In Vitro Fertilization

2006

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International Standard Book Number-10: 0-8493-3335-0 (Hardcover) International Standard Book This book contains information obtained from authentic and highly regarded sources. Reprinted material is quoted with permission, and sources are indicated. A wide variety of references are listed. Reasonable efforts have been made to publish reliable data and information, but the author and the publisher cannot assume responsibility for the validity of all materials or for the consequences of their use.

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High continuing pregnancy rates after in vitro fertilization-embryo transfer using medium supplemented with a plasma protein fraction containing α- and β-globulins

Cited by 65 publications

References 19 publications

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Prospective Randomized Comparison of Two Embryo Culture Systems: P1 Medium by Irvine Scientific and the Cook IVF Medium

In Vitro Fertilization

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