2012
DOI: 10.3791/3452
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High Content Screening in Neurodegenerative Diseases

Abstract: The functional annotation of genomes, construction of molecular networks and novel drug target identification, are important challenges that need to be addressed as a matter of great urgency [1][2][3][4] . Multiple complementary 'omics' approaches have provided clues as to the genetic risk factors and pathogenic mechanisms underlying numerous neurodegenerative diseases, but most findings still require functional validation 5. For example, a recent genome wide association study for Parkinson's Disease (PD), ide… Show more

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Cited by 7 publications
(5 citation statements)
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“…Cell-based “phenotypic” high-throughput screening (HTS) is a powerful unbiased tool to identify gene targets or small-molecule compounds exerting desired effects. However, HTS requires large numbers of cells and has been largely restricted to immortalized human neuronal lines, such as neuroblastoma SH-SY5Y( Jain et al., 2012 ) and glioma H4 ( Albrecht et al., 2004 ) cells, or non-neuronal lines, such as HeLa cells ( Fatokun et al., 2013 ). Since these cells differ physiologically from post-mitotic neurons, hits identified in these cells might not work in neurons.…”
Section: Introductionmentioning
confidence: 99%
“…Cell-based “phenotypic” high-throughput screening (HTS) is a powerful unbiased tool to identify gene targets or small-molecule compounds exerting desired effects. However, HTS requires large numbers of cells and has been largely restricted to immortalized human neuronal lines, such as neuroblastoma SH-SY5Y( Jain et al., 2012 ) and glioma H4 ( Albrecht et al., 2004 ) cells, or non-neuronal lines, such as HeLa cells ( Fatokun et al., 2013 ). Since these cells differ physiologically from post-mitotic neurons, hits identified in these cells might not work in neurons.…”
Section: Introductionmentioning
confidence: 99%
“…In a study using SH-SY5Y neuronal cells, lentiviral shRNA was used to infect dividing cells that were subsequently differentiated, with multiple assays applied to the resulting neurons, including neurite-outgrowth and mitochondrial function. 50 Pooled shRNA screening has been increasingly popular in recent years, driven in part by not requiring the expensive and bulky automation needed for genome-scale arrayed screens. 51 Pooled screens also have a distinct advantage over arrayed screens when studying genes affecting cell proliferation over multiple doublings (and lasting perhaps weeks in length) due to space constraints imposed for cell growth by well geometry and the difficulty in refeeding cells in the plates.…”
Section: Screening Technologiesmentioning
confidence: 99%
“…In a study using SH-SY5Y neuronal cells, lentiviral shRNA was used to infect dividing cells that were subsequently differentiated, with multiple assays applied to the resulting neurons, including neurite-outgrowth and mitochondrial function. 50…”
Section: Screening Technologiesmentioning
confidence: 99%
“…Currently, the size-scope tradeoff is inevitable. However, as the efforts towards high-quality network curation gain community-scale attention [ 47 , 179 ], and new high-content screening approaches are proposed for network construction [ 180 ], we may expect that disease-specifi c networks will grow in size without sacrifi cing their quality.…”
Section: Synthesismentioning
confidence: 99%