High-Content Imaging-Based Assay for SARS-CoV-2-Neutralizing Antibodies
Vinícius Pinto Costa Rocha,
Bruna Aparecida Souza Machado,
Helenita Costa Quadros
et al.
Abstract:The COVID-19 pandemic and the consequent emergence of new SARS-CoV-2 variants of concern necessitates the determination of populational serum potency against the virus. Here, we standardized and validated an imaging-based method to quantify neutralizing antibodies against lentiviral particles expressing the spike glycoprotein (pseudovirus). This method was found to efficiently quantify viral titers based on ZsGreen-positive cells and detect changes in human serum neutralization capacity induced by vaccination … Show more
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