High‐Content Image‐Based Screening and Deep Learning for the Detection of Anti‐Inflammatory Drug Leads
Tannia A. Lau,
Elmar Mair,
Beverley M. Rabbitts
et al.
Abstract:We developed a high‐content image‐based screen that utilizes the pro‐inflammatory stimulus lipopolysaccharide (LPS) and murine macrophages (RAW264.7) with the goal of enabling the identification of novel anti‐inflammatory lead compounds. We screened 2,259 bioactive compounds with annotated mechanisms of action (MOA) to identify compounds that block the LPS‐induced phenotype in macrophages. We utilized a set of seven fluorescence microscopy probes to generate images that were used to train and optimize a deep n… Show more
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