High content analysis methods enable high throughput nematode discovery screening for viability and movement behavior in a multiplex sample in response to natural product treatment

Abstract

Monitoring nematode parasite movement and mortality in response to various treatment samples usually involves tedious manual microscopic analysis. High Content Analysis instrumentation enables rapid and high throughput collecting of large numbers of treatment data on huge numbers of individual worms. These large sample sizes and increased sample diversity result in robust, reliable results with increased statistical significance. These methods would be applicable to relevant human, crop, or animal worm parasites.