High concentration of trichlorfon (1 mM) disrupts axonal cytoskeleton and decreases the expression of plasticity-related proteins in SH-SY5Y cells

Fernandes, Liliam; Emerick, Guilherme Luz; Ferreira, Rafaela Scalco; Santos, Neife Aparecida Guinaim dos; Santos, Antônio Cardozo dos

doi:10.1016/j.tiv.2016.12.003

Cited by 10 publications

(12 citation statements)

References 69 publications

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“…SH-SY5Y cells express both synaptic proteins, among others, and reduced levels of these synaptic proteins is expected to alter the cells function. Several studies reported that Aβo lead to down-regulation in synaptic markers (Leuba et al, 2008, Feng et al, 2014, Fernandes et al, 2017), and the loss or modification of synaptic proteins directly affects their properties, ultimately impacting synaptic function (Antonucci et al, 2016). …”

Section: Discussionmentioning

confidence: 99%

Oleocanthal ameliorates amyloid-β oligomers’ toxicity on astrocytes and neuronal cells: In vitro studies

et al. 2017

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Extra-virgin olive oil (EVOO) has several health promoting effects. Evidence have shown that EVOO attenuates the pathology of amyloid-β (Aβ) and improves cognitive function in experimental animal models, suggesting it’s potential to protect and reduce the risk of developing Alzheimer’s disease (AD). Available studies have linked this beneficial effect to oleocanthal, one of the active components in EVOO. The effect of oleocanthal against AD pathology has been linked to its ability to attenuate Aβ and tau aggregation in vitro, and enhance Aβ clearance from the brains of wild type and AD transgenic mice in vivo. However, the ability of oleocanthal to alter the toxic effect of Aβ on brain parenchymal cells is unknown. In the current study, we investigated oleocanthal effect on modulating Aβ oligomers (Aβo) pathological events in neurons and astrocytes. Our findings demonstrated oleocanthal prevented Aβo-induced synaptic proteins, SNAP-25 and PSD-95, down-regulation in neurons, and attenuated Aβo-induced inflammation, glutamine transporter (GLT1) and glucose transporter (GLUT1) down-regulation in astrocytes. Aβo-induced inflammation was characterized by interleukin-6 (IL-6) increase and glial fibrillary acidic protein (GFAP) upregulation that were reduced by oleocanthal. In conclusion, this study provides further evidence to support the protective effect of EVOO-derived phenolic secoiridoid oleocanthal against AD pathology.

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Section: Discussionmentioning

confidence: 99%

Oleocanthal ameliorates amyloid-β oligomers’ toxicity on astrocytes and neuronal cells: In vitro studies

et al. 2017

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“…Increases in astrocyte density due to subchronic OP-induced toxicity have been reported in murine models, in which GFAP was used as a marker of reactivity [47], similar to the findings of the present study. This increase in reactivity has been associated with the neuroprotective role of glial cells against OP toxicity [46] and can be a consequence of the inhibition of neuropathy target esterase [48], eliciting an imbalance in the levels of lysophosphatidylcholine in the neuron cell membrane, which, in turn, brings about cytotoxicity, characterized by chronic demyelination [49,50].…”

Section: Discussionmentioning

confidence: 99%

First study on response of astrocytes in alevines of red-bellied pacu (Piaractus brachypomus) to subchronic exposure to chlorpyrifos and trichlorfon

2022

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Background and Aim: Organophosphate pesticides (OPs) used in agricultural production pose environmental and public health risks whenever non-target organisms are exposed to them. Oxon-type OPs, such as trichlorfon (TCF) and chlorpyrifos (CPF), are frequently used in Colombia and have been detected in water bodies in the vicinity of croplands; however, their effect on aquatic organisms, especially fish, is largely unknown. The neurotoxicity of OPs includes inhibition of esterase enzymes, neuronal damage, and increased glial reactivity. This study aimed to assess the astrocytic response in the brain tissue of juvenile red-bellied pacu (Piaractus brachypomus) exposed to TCF and CPF. Materials and Methods: A 25-day subchronic assay was conducted in which juvenile red-bellied pacu were exposed to CPF and TCF. After 25 days of exposure, the fish were killed and brain samples were collected and processed for immunohistochemistry to assess the morphology and reactivity of astrocytes; glial acidic fibrillary protein was used as a biomarker. Results: The brain samples from animals under subchronic exposure to OPs for 25 days showed higher cellular density as well as changes in astrocyte phenotype characterized by shortening of cytoplasmic projections, hypertrophy, and ameboid morphology compared to those from nonexposed animals. Similarly, astrocyte hyperreactivity was detected in the optic tectum and medial longitudinal fasciculus of the exposed group. Conclusion: Immunoreactivity of brain glial cells under subchronic exposure to OPs measured through immunohistochemical tests as well as OPs-induced neuropathology may be useful as a biomarker for monitoring environmental pollution. The results also indicate that P. brachypomus is a suitable biomonitoring model for studying neurotoxicological and neurodegenerative diseases.

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“…Finally, the increased popularity of insecticides such as fipronil and pyrethroids make them also an interesting object of study, with 7 articles for fipronil, 7 more for cypermethrin and 6 for deltamethrin for instance. Differentiated cells were preferentially used in neuron-specific endpoints that need phenotypic characteristic of mature neurons such as cell membrane potential [51,53], acetylcholine receptor (AChR) signaling [51][52][53], voltage-gated calcium channel (VOCC) flux [51][52][53], intracellular calcium homeostasis [51,52,58], neurite outgrowth [51,52,[57][58][59][60][61], and expression of genes involved in functional properties of neuronal cells such as the gene encoding TH, an enzyme involved in dopaminergic function [62]. Finally, in some studies that analyzed the activities of acetylcholine and neuropathy target esterases (AChE, NTE), cells were differentiated to increase expression of these enzymes [63][64][65][66]58].…”

Section: Pesticidesmentioning

confidence: 99%

“…Therefore, these endpoints are also studied when assessing the neuropathic potential of a compound. Paraoxon can cause a decrease in neurite length, but not in the number of cells with neurites, and in the amount of cytoskeleton proteins growth-associated protein 43 (GAP-43), synapsin I, synaptophysin and NEFH, although its effects seem to be milder than those of neuropathic OPs [60]. At the concentrations that inhibit 70% NTE, paraoxon causes a decrease in cell viability and has no effect on calpain activity and intracellular calcium homeostasis [58,59].…”

Section: -Impact Of Ops On Other Features Associated With Neuropathymentioning

confidence: 99%

The SH-SY5Y human neuroblastoma cell line, a relevant in vitro cell model for investigating neurotoxicology in human: Focus on organic pollutants

et al. 2022

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High concentration of trichlorfon (1 mM) disrupts axonal cytoskeleton and decreases the expression of plasticity-related proteins in SH-SY5Y cells

Cited by 10 publications

References 69 publications

Oleocanthal ameliorates amyloid-β oligomers’ toxicity on astrocytes and neuronal cells: In vitro studies

Oleocanthal ameliorates amyloid-β oligomers’ toxicity on astrocytes and neuronal cells: In vitro studies

First study on response of astrocytes in alevines of red-bellied pacu (Piaractus brachypomus) to subchronic exposure to chlorpyrifos and trichlorfon

The SH-SY5Y human neuroblastoma cell line, a relevant in vitro cell model for investigating neurotoxicology in human: Focus on organic pollutants

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