High concentration of residual aluminum oxide on titanium surface inhibits extracellular matrix mineralization

Abstract

In the present study we characterized titanium (Ti) surfaces submitted to different treatments and evaluated the response of osteoblasts derived from human alveolar bone to these surfaces. Five different surfaces were evaluated: ground (G), ground and chemical etched (G1-HF for 60 s), sand blasted (SB-Al(2)O(3) particles 65 mum), sand blasted and chemical etched (SLA1-HF for 60 s and SLA2-HF for 13 s). Surface morphology was evaluated under SEM and roughness parameters by contact scanning instrument. The presence of Al(2)O(3) was detected by EDS and the amount calculated by digital analyses. Osteoblasts were cultured on these surfaces and it was evaluated: cell adhesion, proliferation, and viability, alkaline phosphatase activity, total protein content, and matrix mineralization formation. Physical and chemical treatments produced very different surface morphologies. Al(2)O(3) residues were detected on SB and SLA2 surfaces. Only matrix mineralization formation was affected by different surface treatments, being increased on rough surface (SLA1) and reduced on surface with high amount of Al(2)O(3) residues (SB). On the basis of these findings, it is possible to conclude that high concentration of residual Al(2)O(3) negatively interfere with the process of matrix mineralization formation in contact with Ti implant surfaces.

Two‐ and three‐dimensional profilometer assessments to determine titanium roughness

“…The topography of Ti surfaces must be extensively analyzed using the classical amplitude and frequency parameters and also other parameters describing the surface organization (Bigerelle et al 2002). Different parameters have been recommended for 3D characterization of surface roughness (Canabarro et al 2008;Wennerberg et al 1998). The current results confirmed that Ra may not be sufficient in describing the surface morphology.…”

“…These methods include scanning electron microscopy (SEM), confocal laser scanning microscopy, tactile microscopy, optical profilometry, scanning force microscopy, contact scanning instruments and noncontact laser profilometry (Al-Nawas et al 2001;Bigerelle et al 2002;Canabarro et al 2008).…”

Atomic layer deposition of TiO2 thin films on nanoporous alumina templates: Medical applications

“…10 In fact, TiO 2 is commonly used as a passivation layer in dental, orthopedic, and cardiovascular implants. [11][12][13] Having a conformal coating of TiO 2 over the alumina membrane is important to minimize corrosion and improve cell compatibility. Previous work by A. Canabarro et al has indicated that cells grown on TiO 2 surfaces contaminated by small amounts of alumina exhibit impaired activity; for example, contamination by alumina may lead to impaired mineralization of matrix by osteoblasts (bone cells).…”

Fatigue behavior of Ti6Al4V and 316 LVM blasted with ceramic particles of interest for medical devices

“…Besides the biological effects of remnants of aluminum oxide on grit-blasted surfaces (Canabarro et al, 2008), implications of this surface modification in the fatigue behavior, which is the main cause of premature failure of metallic implants, become relevant. This investigation has shown that fatigue resistance increases when blasting with zirconia particles but considerably decreases when using alumina ones.…”