High concentration measurement of mixed particle suspensions using simple multi-angle light scattering system

Abstract

A simple multiple-angle light scattering system was developed for the differential measurement of particle concentrations in suspension even in high concentration where multiple scattering effects are significant based on size. The system combines multiple-angle detection to collect scattered angle dependent light intensities, and Partial Least Square Regression method (PLS-R) to compose the predictive models for analyzing scattered signal obtain concentrations of samples under investigation. The system was designed to be simple, portable and inexpensive. It employs a diode lasers (red AlGaInP-based) as a light source and a silicon photodiode as a detector and optical components, all of which are readily available. The technique was validated using 1.1 μm and 3.0μm polystyrene latex beads in both mono-dispersed and poly-dispersed suspensions. The measurement results showed good agreement between the measured results and reference values. Their deviations from the reference values are 2.4% and 1.5% relating to references' concentrations of 8 10 3 .

Autoencoder-aided measurement of concentration from a single line of speckle

“…There is a substantial literature linking the statistics of the speckle pattern with the size, refractive index and concentration of the scattering particles, or more generally with the properties of the phase screen generating the speckle; see, e.g., [1][2][3][4][5][6][7][8][9] and the references therein. While there are many alternative optical methods for the estimation of properties of particle ensembles [10,11], in this paper we focus on speckle measurement. Speckle analysis presents a non-invasive and potentially fast and accurate technique for determination of concentration of different living cell types used in various diagnostic procedures in medicine and research protocols in biomedical and biotech industry.…”