High-cell-density cultivation of the flagellate alga Poterioochromonas malhamensis for biomanufacturing the water-soluble β-1,3-glucan with multiple biological activities

Ma, Mingyang; Li, Yanhua; Chen, Jianping; Wang, Fuchen; Li, Yuan; Li, Yi; Zhang, Baocai; Ye, Ding; Han, Danxiang; Hu, Jin

doi:10.1016/j.biortech.2021.125447

Cited by 22 publications

(36 citation statements)

References 42 publications

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“…The content of β-1,3-glucans can be extracted or measured by different procedures, such as hot water extraction [ 10 ], alkali extraction [ 36 ], phenol sulfuric acid assay [ 37 ], 3-methyl-2-benzothiazolinone hydrazone (MBTH) reducing sugar assay [ 25 ], enzymatic hydrolysis [ 7 , 8 ], and high-performance size-exclusion chromatography (HPSEC) method [ 38 ]. Among these methods, hot water extraction could achieve the highest amount of β-glucans with high purity and low cost [ 10 ]. However, the optimal extraction method is related with the structure and sources of laminarins [ 39 ].…”

Section: General Features Of Laminarin In Stramenopilesmentioning

confidence: 99%

“…At the same time, nitrogen limitation resulted in the storage of β-1,3-glucan in S. costatum [ 43 , 44 ]. The content of β-1,3-glucan was up to 55% of the cell dry weight under the optimal cultivation conditions in P. malhamensis [ 10 ]. Silicon starvation and lower density culture led to the initial storage of chrysolaminarin in T. pseudonana [ 33 ].…”

Section: General Features Of Laminarin In Stramenopilesmentioning

confidence: 99%

“…The study of chrysolaminarin action mechanism on the cellular and molecular level showed that the biological action of chrysolaminarin was mediated by two membrane receptors, CR3 and Dectin-1 [ 46 ]. The chrysolaminarin of P. malhamensis could promote the fin regeneration of zebra fish by enhancing the antioxidant capacity of the injured zebrafish and reducing the potential damage of ROS to injured zebrafish [ 10 ]. It was verified that the bioactivities of chrysolaminarin are affected by their structure, molecular weight, solubility, and by their number of branch and helical conformation [ 10 ].…”

Section: General Features Of Laminarin In Stramenopilesmentioning

confidence: 99%

“…β-1,3-glucans were mainly composed of glucose and also called laminarin, chrysolaminarin, or mycolaminarin depending on the algal species. β-1,3-glucan in brown algae was termed laminarin, in diatom Phaeodactylum tricornutum , Chrysophyte Poterioochromonas malhamensis and Eustigmatophyceae class Nannochloropsis gaditana was named chrysolaminarin and, in oomycetes, mycolaminarin [ 9 , 10 , 11 , 12 , 13 ]. These β-1,3-glucans are the main carbohydrate molecules in the ocean carbon cycle and carbon pool [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…These β-1,3-glucans are the main carbohydrate molecules in the ocean carbon cycle and carbon pool [ 8 ]. In addition, it was also reported that β-1,3-glucans possess various biological activities and functions in food, cosmetic and pharmaceuticals [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Laminarin, a Major Polysaccharide in Stramenopiles

Chen

Yang

et al. 2021

Marine Drugs

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During the processes of primary and secondary endosymbiosis, different microalgae evolved to synthesis different storage polysaccharides. In stramenopiles, the main storage polysaccharides are β-1,3-glucan, or laminarin, in vacuoles. Currently, laminarin is gaining considerable attention due to its application in the food, cosmetic and pharmaceuticals industries, and also its importance in global biogeochemical cycles (especially in the ocean carbon cycle). In this review, the structures, composition, contents, and bioactivity of laminarin were summarized in different algae. It was shown that the general features of laminarin are species-dependence. Furthermore, the proposed biosynthesis and catabolism pathways of laminarin, functions of key genes, and diel regulation of laminarin were also depicted and comprehensively discussed for the first time. However, the complete pathways, functions of genes, and diel regulatory mechanisms of laminarin require more biomolecular studies. This review provides more useful information and identifies the knowledge gap regarding the future studies of laminarin and its applications.

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Section: General Features Of Laminarin In Stramenopilesmentioning

confidence: 99%

Section: General Features Of Laminarin In Stramenopilesmentioning

confidence: 99%

Section: General Features Of Laminarin In Stramenopilesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Laminarin, a Major Polysaccharide in Stramenopiles

Chen

Yang

et al. 2021

Marine Drugs

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Value-Added Chemicals from Algae: Extraction and Refining

Saggu,

Kumar

2024

Value Added Products From Bioalgae Based Biorefineries: Opportunities and Challenges

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A systematic review of the predatory contaminant Poterioochromonas in microalgal culture

Wei

Huang

et al. 2023

J Appl Phycol

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Contamination by zooplankton has to a certain extent limited the large-scale cultivation and industrial exploitation of microalgae. However, systematic research on these predators in microalgal culture is still lacking. The identification of zooplanktonic contaminants derived from microalgal cultures is a basis for conducting related studies. Moreover, knowledge of the ecological distribution of such predators is crucial for avoiding or reducing the risk of biological contamination in the management of large-scale microalgal cultures. Understanding the feeding behaviors of zooplanktonic contaminants contributes to the establishment of targeted prevention strategies and control methods. Early detection is essential to allow prevention and control measures to be implemented in a timely and effective way. Reducing the susceptibility of the cultured microalgae to predators through breeding strains selection, the potential of modern molecular methods, or a synthesis of these approaches will be indispensable to the management of zooplankton contamination. Furthermore, exploring the resource utilization of predators helps to understand this issue comprehensively and to turn hazard into wealth. The genus Poterioochromonas is a typical mixotrophic flagellate and has attracted increasing attention because of the dramatic damage it can inflict on a wide range of microalgal cultures, regardless of the culture system, season, or environment. This review explores our current understanding of the predator Poterioochromonas and the areas where further research is needed, which should stimulate reflection on what we still need to know about these predators from a microalgal culture perspective and how we can utilize them.

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High-cell-density cultivation of the flagellate alga Poterioochromonas malhamensis for biomanufacturing the water-soluble β-1,3-glucan with multiple biological activities

Cited by 22 publications

References 42 publications

Laminarin, a Major Polysaccharide in Stramenopiles

Laminarin, a Major Polysaccharide in Stramenopiles

Value-Added Chemicals from Algae: Extraction and Refining

A systematic review of the predatory contaminant Poterioochromonas in microalgal culture

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