High cell density cultivation of Corynebacterium glutamicum on bio-based lignocellulosic acetate using pH-coupled online feeding control

Kiefer, Dirk; Merkel, Manuel; Lilge, Lars; Hausmann, Rudolf; Henkel, Marius

doi:10.1016/j.biortech.2021.125666

Cited by 19 publications

(28 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a recent study, lignocellulose-derived acetate was utilized as a sole carbon source and as acid pH titrant, while urea was fed as a nitrogen source. By dynamical adaptation of the C/N feeding ratio, a maximal cell dry weight of 80.2 g L −1 was achieved [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production

et al. 2022

View full text Add to dashboard Cite

Coenzyme Q10 (CoQ10) is a lipid-soluble compound with important physiological functions and is sought after in the food and cosmetic industries owing to its antioxidant properties. In our previous proof of concept, we engineered for CoQ10 biosynthesis the industrially relevant Corynebacterium glutamicum, which does not naturally synthesize any CoQ. Here, liquid chromatography–mass spectrometry (LC–MS) analysis identified two metabolic bottlenecks in the CoQ10 production, i.e., low conversion of the intermediate 10-prenylphenol (10P-Ph) to CoQ10 and the accumulation of isoprenologs with prenyl chain lengths of not only 10, but also 8 to 11 isopentenyl units. To overcome these limitations, the strain was engineered for expression of the Ubi complex accessory factors UbiJ and UbiK from Escherichia coli to increase flux towards CoQ10, and by replacement of the native polyprenyl diphosphate synthase IspB with a decaprenyl diphosphate synthase (DdsA) to select for prenyl chains with 10 isopentenyl units. The best strain UBI6-Rs showed a seven-fold increased CoQ10 content and eight-fold increased CoQ10 titer compared to the initial strain UBI4-Pd, while the abundance of CoQ8, CoQ9, and CoQ11 was significantly reduced. This study demonstrates the application of the recent insight into CoQ biosynthesis to improve metabolic engineering of a heterologous CoQ10 production strain.

show abstract

Section: Discussionmentioning

confidence: 99%

Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Rice straw hydrolyzates supported amino acid production best when both araBAD and xylAB were overexpressed ( Meiswinkel et al, 2013a ). The fact that C. glutamicum readily utilizes acetate as sole and combined source of carbon and energy ( Gerstmeir et al, 2003 ) was exploited for production using blends, e.g., of acetate and xylose ( Mindt et al, 2019b ) or of lignocellulosic acetate ( Kiefer et al, 2021 ).…”

Section: Engineering Corynebacterium Glutamicum Fo...mentioning

confidence: 99%

Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds by Corynebacterium glutamicum

2022

View full text Add to dashboard Cite

show abstract

“…Ethanol is well known as cytotoxin that increases the permeability of the membrane for polar and charged molecules, leading to leakage (Ingram, 1989). However, such drawbacks can be tackled such as done with C. glutamicum via pH-coupled feeding of bio-acetate into the reactor (Kiefer et al, 2021), or with E. coli by rewiring the cAMP receptor protein (Chong et al, 2013) or by consecutive growth selections to increase tolerance (Sandoval et al, 2011). Beyond that, E. coli has been engineered in various ways to improve acetate uptake and productivity with acetate as carbon source (reviewed in Kutscha and Pflügl, 2020).…”

Section: Product Formation From Acetate And/or Ethanol: Potential Org...mentioning

confidence: 99%

The Potential of Sequential Fermentations in Converting C1 Substrates to Higher-Value Products

et al. 2022

View full text Add to dashboard Cite

Today production of (bulk) chemicals and fuels almost exclusively relies on petroleum-based sources, which are connected to greenhouse gas release, fueling climate change. This increases the urgence to develop alternative bio-based technologies and processes. Gaseous and liquid C1 compounds are available at low cost and often occur as waste streams. Acetogenic bacteria can directly use C1 compounds like CO, CO2, formate or methanol anaerobically, converting them into acetate and ethanol for higher-value biotechnological products. However, these microorganisms possess strict energetic limitations, which in turn pose limitations to their potential for biotechnological applications. Moreover, efficient genetic tools for strain improvement are often missing. However, focusing on the metabolic abilities acetogens provide, they can prodigiously ease these technological disadvantages. Producing acetate and ethanol from C1 compounds can fuel via bio-based intermediates conversion into more energy-demanding, higher-value products, by deploying aerobic organisms that are able to grow with acetate/ethanol as carbon and energy source. Promising new approaches have become available combining these two fermentation steps in sequential approaches, either as separate fermentations or as integrated two-stage fermentation processes. This review aims at introducing, comparing, and evaluating the published approaches of sequential C1 fermentations, delivering a list of promising organisms for the individual fermentation steps and giving an overview of the existing broad spectrum of products based on acetate and ethanol. Understanding of these pioneering approaches allows collecting ideas for new products and may open avenues toward making full use of the technological potential of these concepts for establishment of a sustainable biotechnology.

show abstract

High cell density cultivation of Corynebacterium glutamicum on bio-based lignocellulosic acetate using pH-coupled online feeding control

Cited by 19 publications

References 38 publications

Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production

Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production

Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds by Corynebacterium glutamicum

The Potential of Sequential Fermentations in Converting C1 Substrates to Higher-Value Products

Contact Info

Product

Resources

About