High Catalytic Activity of Lipase from Yarrowia lipolytica Immobilized by Microencapsulation

Abstract

Microencapsulation of lipase from Yarrowia lipolytica IMUFRJ 50682 was performed by ionotropic gelation with sodium alginate. Sodium alginate, calcium chloride and chitosan concentrations as well as complexation time were evaluated through experimental designs to increase immobilization yield (IY) and immobilized lipase activity (ImLipA) using p-nitrophenyl laurate as substrate. To adjust both parameters (IY and ImLipA), the desirability function showed that microcapsule formation with 3.1%(w/v) sodium alginate, 0.19%(w/v) chitosan, 0.14 M calcium chloride, and 1 min complexation time are ideal for maximal immobilization yield and immobilized lipase activity. A nearly twofold enhancement in Immobilization yield and an increase up to 280 U/g of the lipase activity of the microcapsules were achieved using the experimental design optimization tool. Chitosan was vital for the high activity of this new biocatalyst, which could be reused a second time with about 50% of initial activity and for four more times with about 20% of activity.

Use of Yarrowia lipolytica Lipase Immobilized in Cell Debris for the Production of Lipolyzed Milk Fat (LMF)

“…Cell-free medium, after 24 h fermentation, contained approximately 4700 U/L of extracellular lipase activity. The extracellular lipase extract was used for other studies [19]. At this moment 5.7 g d.w. (dry weigh) cells/L was detected in the culture medium, resulting in, approximately 17 g d.w. of cells.…”

Purification, Characterization and Immobilization of Lipase from Proteus vulgaris OR34 for Synthesis of Methyl Oleate

“…Indeed, the immobilization is a technique which consists to the attachment of enzymes to solid support materials in order to improve their activity, stability and reusability for a continuous process [12]. Several studies reported the different lipases immobilization techniques such as physical adsorption [13], entrapment or microencapsulation [14,15] and covalence binding to a solid matrix [16]. Among these techniques, adsorption may have the highest interest due to its relatively low cost and simplicity [17].…”

Recent Trends in Biomaterials for Immobilization of Lipases for Application in Non-Conventional Media

“…A disadvantage of this support is that small proteins may leach into the bulk medium. For example, a crude extract containing lipases of Yarrowia lipolytica was immobilized by microencapsulation in alginate [100]. The immobilization itself was successful, with the immobilized biocatalyst initially having high hydrolytic activity against p-nitrophenyl laurate; however, 50% of the activity had leached out of the alginate beads by the end of the reaction [100].…”

“…For example, a crude extract containing lipases of Yarrowia lipolytica was immobilized by microencapsulation in alginate [100]. The immobilization itself was successful, with the immobilized biocatalyst initially having high hydrolytic activity against p-nitrophenyl laurate; however, 50% of the activity had leached out of the alginate beads by the end of the reaction [100]. The optimization of immobilization parameters can reduce the leaching of small proteins, for example, by controlling the amount of alginate and the concentration of Ca 2+ to fine tune the gel properties [101].…”