High cardiomyocyte diversity in human early prenatal heart development

Sylvén, Christer; Wärdell, Eva; Månsson‐Broberg, Agneta; Cingolani, Eugenio; Ampatzis, Konstantinos; Larsson, Ludvig; Björklund, Åsa K.; Giacomello, Stefania

doi:10.1016/j.isci.2022.105857

Cited by 5 publications

(5 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To bridge this gap, we performed fine-grained clustering of the cardiomyocyte population and identified 19 distinct cell states, confirming the recently reported transcriptional diversity of these cells 36 . Besides a range of contractile cardiomyocytes of ventricular (vCM_1-6) and atrial origins (Left_aCM, Right_aCM, and Cond_aCM, representing smooth-walled conduit atrial regions), we also recognized cell states of lower maturation levels (Immat_CM_1-3), and of cell cycle signature (Prol_CM_1-2) (Fig.…”

Section: Molecular Map Of the Cardiac Pacemaker-conduction System Dev...supporting

confidence: 69%

Spatial Dynamics of the Developing Human Heart

Lázár,

Mauron,

Andrusivová

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Heart development relies on a topologically defined interplay between a diverse array of cardiac cells. We finely curated spatial and single-cell measurements with subcellular imaging-based transcriptomics validation to explore spatial dynamics during early human cardiogenesis. Analyzing almost 80,000 individual cells and 70,000 spatially barcoded tissue regions between the 5.5th and 14th postconceptional weeks, we identified 31 coarse- and 72 fine-grained cell states and mapped them to highly resolved cardiac cellular niches. We provide novel insight into the development of the cardiac pacemaker-conduction system, heart valves, and atrial septum, and decipher heterogeneity of the hitherto elusive cardiac fibroblast population. Furthermore, we describe the formation of cardiac autonomic innervation and present the first spatial account of chromaffin cells in the fetal human heart. In summary, our study delineates the cellular and molecular landscape of the developing heart's architecture, offering links to genetic causes of heart disease.

show abstract

Section: Molecular Map Of the Cardiac Pacemaker-conduction System Dev...supporting

confidence: 69%

Spatial Dynamics of the Developing Human Heart

Lázár,

Mauron,

Andrusivová

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“… 28 Similarly, transcriptome profiling of mammalian cardiomyocytes at the single cell level have revealed unprecedented molecular diversity which seems to suggest further functional specializations and/or distinct energetic profiles within this cell type. 25 , 65 , 107 A specific population of cardiomyocytes that express Myoz2, a member of the sarcomeric protein family that is implicated in hypertrophic response, 108 was recently described in mouse and humans. 26 , 65 We observed that myoz2b, a zebrafish ortholog of this gene was expressed in the zebrafish myocardium.…”

Section: Discussionmentioning

confidence: 99%

scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular players in heart rhythm regulation

Abu Nahia,

Sulej,

Migdał

et al. 2024

iScience

View full text Add to dashboard Cite

“…Previous single cell analyses on the developing epicardium in zebrafish has uncovered distinct subpopulations, each possessing specific genetic signatures and function [28]. Similarly, transcriptome profiling of mammalian cardiomyocytes at the single cell level have revealed unprecedented molecular diversity which seems to suggest further functional specializations and/or distinct energetic profiles within this cell type [25,85,122]. A specific population of cardiomyocytes that express Myoz2, a member of the sarcomeric protein family that is implicated in hypertrophic response [123], was recently described in mouse and humans [26,85].…”

Section: Discussionmentioning

confidence: 99%

scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular building blocks of the primary pacemaker

Nahia

Sulej

Migdał

et al. 2023

Preprint

View full text Add to dashboard Cite

The heart is comprised of a variety of specialized cell types that work in unison to maintain blood flow. Here we utilized scRNA-seq analysis to delineate the diversity of cardiac cell types in the zebrafish. With the growing use of the zebrafish to model human heart biology, a deeper insight into its complex cellular composition is critical for a better understanding of heart function, development, and associated malformations. We present a high resolution atlas of zebrafish heart single cells transcriptomics, consisting of over 50 000 cells representing the building blocks of the zebrafish heart at 48 and 72 hpf. We defined 18 discrete cell populations comprising major cell lineages and sublineages of the developing heart. We pinpointed a population of cells likely to be the primary pacemaker and identified the transcriptome profile defining this critical cell type. Our analyses identified two genes, atp1b3b and colec10, which were enriched in the sinoatrial pacemaker cells. CRISPR/Cas9-mediated knockout of these two genes significantly reduced heart rate which is accompanied by arrhythmia or morphological defects, suggesting their novel function in cardiac development and conduction. Additionally, we describe other subpopulations of cardiac cell lineages, including the endothelial and neural cells, whose expression profiles we provide as a resource for further investigations into the cellular and molecular mechanisms of this organ.

show abstract

High cardiomyocyte diversity in human early prenatal heart development

Cited by 5 publications

References 70 publications

Spatial Dynamics of the Developing Human Heart

Spatial Dynamics of the Developing Human Heart

scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular players in heart rhythm regulation

scRNA-seq reveals the diversity of the developing cardiac cell lineage and molecular building blocks of the primary pacemaker

Contact Info

Product

Resources

About