High-capacity boronate affinity-based template-immobilized surface imprinted silica nanoparticles for rapid, selective, and efficient extraction and determination of lincomycin in milk and chicken

Abstract

BackgroundLincomycin, a natural antibiotic, is widely used by animal and fishery husbandries to prevent infections and treat diseases. It endangers people’s health when they eat foods containing lincomycin residue, especially the frequent consumption of milk and chicken products containing lincomycin. Hence, it is extremely important to evaluate the content of lincomycin in food samples. However, a direct analysis of lincomycin in milk and chicken is quite difficult because of its very low concentration level and the presence of undesirable matrix effects. Therefore, selective and efficient extraction of lincomycin from complex food samples prior to its quantification is required.ResultsIn this study, lincomycin-imprinted silica nanoparticles were prepared according to boronate affinity-based template-immobilized surface imprinting. Silica nanoparticles and boronic acid ligands 3-fluoro-4-formylphenylboronic acid were used as supporting materials and functional monomers, respectively. The prepared lincomycin-imprinted silica nanoparticles exhibited several significant results, such as good specificity, high binding capacity (19.45 mg/g), fast kinetics (6 min), and low binding pH (pH 5.0) toward lincomycin. The reproducibility of lincomycin-imprinted silica nanoparticles was satisfactory. The lincomycin-imprinted silica nanoparticles could still be reused after seven adsorption–desorption cycles, which indicated high chemical stability. In addition, the recoveries of the proposed method for lincomycin at three spiked levels of analysis in milk and chicken were 93.3–103.3% and 90.0–100.0%, respectively.ConclusionThe prepared lincomycin-imprinted silica nanoparticles are feasible for the recognition of target lincomycin with low concentrations in real food samples such as milk and chicken. Our approach makes sample pre-preparation simple, fast, selective, and efficient.

The preparation of a boronate affinity-based controlled oriented imprinting coating on a silica nanoparticle surface for the separation and purification of shikimic acid in herbal medicine

“…Clearly, such equilibrium time of SiO 2 @FFPBA@MIPs for SA was lower than that of other imprinted polymers (8–90 min). 15,28–31 This result indicated that SiO 2 @FFPBA@MIPs for SA showed good binding kinetics.…”

“…The p K a value of FFPBA was reported to be approximately 5.8 due to the presence of electron-withdrawing groups in FFPBA. 28 Clearly, this kind of boronic acid ligand could work under low pH conditions of 6.0 (see Fig. 6).…”

Study on the creation of boronate affinity-based oriented imprinted silica nanoparticles and their selective recognition toward glycopeptide antibiotics in food and water

“…The p K a value of FFPBA was reported to be approximately 5.8 due to the presence of electron-withdrawing groups in FFPBA. 36 Clearly, this kind of boronic acid ligands could work under low pH conditions of 6.0 (see Fig. 8).…”

“…Silica nanoparticles (SiO 2 ), due to their good biocompatibility, low toxicity, easy post modification, high yield synthesis and easy preparation, can be well used as solid substrates. 33–36 Thus, high-density FFPBA ligands can be formed on the surface of SiO 2 by post-modification reactions. Additionally, the imprinting coating plays a crucial role in determining the binding characteristics of imprinted materials.…”

Synthesis of Boronate Affinity-Based Oriented Dummy Template-Imprinted Magnetic Nanomaterials for Rapid and Efficient Solid-Phase Extraction of Ellagic Acid from Food

“…The structures of the boronic acid ligands and supporting materials determine the binding affinity [ 21 ]. Due to the presence of electron-withdrawing groups, the FFPBA used exhibited a low p K a value of 5.8 [ 49 ]. The boronic acid ligand was able to work at a relatively low pH condition of 6.0 ( Figure 8 ).…”