High calpain-1 expression predicts a poor clinical outcome and contributes to tumor progression in pancreatic cancer patients

Yu, L M; Zhu, Yuesheng; Xu, Changzhao; Zhou, Lingling; Xue, Zhanxiong; Cai, Zhenzhai

doi:10.1007/s12094-018-02006-6

Cited by 14 publications

(9 citation statements)

References 23 publications

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“…Вероятно, при увеличении выборки больных РГ могут быть получены значимо ассоциированные результаты. Возможно, уровень экспрессии и значимые корреляции кальпаинов 1 и 2 с клинико-морфологическими характеристиками зависят от других характеристик опухоли [10] или связаны с важной ролью других членов семейства кальпаинов в патогенезе РГ [11,12].…”

Section: результаты и обсуждениеunclassified

“…высоким уровнем экспрессии EZR, что косвенно может указывать на активацию процессов инвазии [9,10]. В целом, результаты подтверждаются данными о тканеспецифичности кальпаинов, например о связи рака гортани с CAPN10 [11], а уровень экспрессии CAPN6 отрицательно коррелирует с исходом заболевания у больных плоскоклеточным раком головы и шеи [12].…”

Section: результаты и обсуждениеunclassified

“…На представленной выборке больных РГ нами обнаружено, что уровень экспрессии CAPN1 связан с уровнем экспрессии генов белков цитоскелета -ви-ментина и САР1, участвующих в ремоделировании цитоскелета. Уровень экспрессии CAPN2 коррелировал с экспрессией мРНК эзрина, белка-линкера между плазматической мембраной и актиновым цитоскелетом, высокий уровень которого ассоциирован с инвазивным ростом [10]. Лимфогенное метастазирование у больных РГ коррелировало с высоким уровнем экспрессии CAPN1, уровень CAPN2 не имел такой связи.…”

Section: заключениеunclassified

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Relationship between the mRNA expression levels of calpains 1/2 and proteins involved in cytoskeleton remodeling

et al. 2020

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Remodeling of the cytoskeleton underlies various cellular processes, including those associated with metastasis. The role of the proteases and proteins involved in cytoskeletal reorganization is being actively studied. However, there are no published data on the relationship between the mRNA expression levels of calpains 1/2 (CAPN 1/2) and the proteins associated with cytoskeleton remodeling. Therefore, the purpose of our study was to establish the relationship between the mRNA expression levels of CAPN 1/2 and the proteins involved in cytoskeletal reorganization, such as cell motility markers (SNAI1, VIM, and RND3) and actin-binding proteins (CFN1, PFN1, EZR, FSCN1, and CAP1) using the model of laryngeal/laryngopharyngeal squamous cell carcinoma (LC). The gene expression level was determined by reverse transcriptase real-time PCR and calculated using the 2-Ct method in paired tissue samples of 44 patients with LC (T1-4N0-2M0). The patients were divided into two groups: those with low and those with high CAPN 1/2 expression levels. It was found that metastasis in LC patients was associated with decreased expression levels of VIM and CAP1, and increased levels of CAPN1. A high level of CAPN2 was accompanied by a high expression level of EZR, indicating the activation of invasion processes. The results obtained need to be confirmed in further studies using a larger sample of patients and target genes. Our study is important in elucidating the mechanisms that underlie cancer progression and metastasis, a development that could subsequently open the way to a search for new prognostic and predictive markers of laryngeal/laryngopharyngeal cancer progression.

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Section: результаты и обсуждениеunclassified

Section: заключениеunclassified

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Relationship between the mRNA expression levels of calpains 1/2 and proteins involved in cytoskeleton remodeling

et al. 2020

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Section: Introductionmentioning

confidence: 99%

“…Earlier studies have shown that some CAPNs are closely related to PC (21)(22)(23). For instance, a study by Yu et al (22) showed that CAPN1 expression was higher in tumor tissue than that in normal tissue, and overexpressed CAPN1 was associated with advanced tumor stage and tumor metastasis. Otherwise, this study also showed that CAPN1 overexpression was correlated with poor prognosis, which can promote tumor cell proliferation, invasion, and migration in in vitro study.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of prognostic value and immune infiltration of calpains in pancreatic cancer

Lv¹,

Tang²,

Liu³

et al. 2021

J Gastrointest Oncol

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Background: Calpains (CAPNs) are intracellular calcium-activated neutral cysteine proteinases involved in cancer initiation, progression, and metastasis. However, its role in pancreatic cancer (PC) is still unclear.This study aims to identify the prognostic value and immune infiltration of CAPNs for PC patients using comprehensive bioinformatics analyzes. Methods: We analyzed the transcription levels of CAPNs in different cancers from Oncomine, differential gene expression in tumor/normal tissues and pathological stage through GEPIA database, the prognostic value of the mRNA expression of CAPNs by Kaplan-Meier plotter, the protein expression comparison of different CAPNs in human tumor/normal tissues from The Human Protein Atla, the CAPNs gene alterations through cBioPortal, the prediction of protein-protein interactions by STRING and GeneMANIA, the functional enrichment of discrepant CAPNs by GO and KEGG, and the immune infiltration of CAPNs by ssGSEA.Results: Our results showed that CAPN1, 2, 4, 5, 6, 8, 9, 10, and 12 were highly expressed in PC. CAPN1, 5, 8, and 12 expression levels were positively correlated with individual cancer stages. Furthermore, CAPN1, 2, 5, and 8 expression levels were negatively correlated with overall survival (OS) and recurrence-free survival (RFS), while CAPN10 was positively correlated with OS and RFS. We found that CAPN1, 2, 5, and 8 were correlated with tumor-infiltrating T follicular helper cells and CAPN10 with tumor-infiltrating T helper 2 cells. Functional enrichment analysis showed that differentially expressed CAPNs (CAPN1, 2, 5, 8, and 10) are involved in axonogenesis, cell-substrate adhesion, immune response-activating cell surface receptor signaling pathway, and cell junction organization in PC.Conclusions: These results suggested that CAPN1, 2, 5, 8, and 10 could be used as prognostic biomarkers in PC and improve individualized treatment strategies.

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Knockout of calpain‐1 protects against high‐fat diet‐induced liver dysfunction in mouse through inhibiting oxidative stress and inflammation

Hao

Zhang²,

et al. 2020

Food Science & Nutrition

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The present study was designed to investigate the significance of calpain‐1 in the high‐fat diet (HFD)‐induced liver dysfunction and to explore the possible mechanism. C57 mice and calpain‐1 knockout (KO) mice were fed with standard diet (SD) or HFD, respectively, for 16 weeks. The activities of calpain, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and superoxide dismutase (SOD) in serum and/or liver of mouse were measured. Lipid profiles in the serum and liver were examined. Contents of oxidized low‐density lipoprotein (oxLDL), malondialdehyde (MDA), tumor necrosis factor (TNF‐α), and interleukin‐6 (IL‐6) in serum or/and liver were detected. The results showed that compared with C57 mice fed with SD, HFD‐fed C57 mice showed the increased activities of AST and ALT in the serum, which was decreased in calpain‐1 KO mice fed with HFD. In addition, knockout of calpain‐1 decreased the contents of oxLDL, MDA, TNF‐α, and IL‐6, while increased SOD activity, in serum and/or liver. However, knockout of calpain‐1 had no effects on lipid profiles in both serum and liver. When fed with SD, all these parameters of C57 and calpain‐1 KO mice were comparable except for decreased calpain activity in the liver of calpain‐1 KO mice. The results suggested that knockout of calpain‐1 protects against HFD‐induced liver dysfunction through inhibiting oxidative stress and inflammation.

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High calpain-1 expression predicts a poor clinical outcome and contributes to tumor progression in pancreatic cancer patients

Cited by 14 publications

References 23 publications

Relationship between the mRNA expression levels of calpains 1/2 and proteins involved in cytoskeleton remodeling

Relationship between the mRNA expression levels of calpains 1/2 and proteins involved in cytoskeleton remodeling

Comprehensive analysis of prognostic value and immune infiltration of calpains in pancreatic cancer

Knockout of calpain‐1 protects against high‐fat diet‐induced liver dysfunction in mouse through inhibiting oxidative stress and inflammation

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