High calcium transport by Polycystin-2 (TRPP2) induces channel clustering and oscillatory currents

Velázquez, Irina Florencia; Cantiello, Horacio F.; Cantero, María del Rocío

doi:10.1016/j.bbrc.2023.03.067

Cited by 1 publication

(1 citation statement)

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“…The breadth and different role in the response to mechanical signals, from shear stress to blood pressure, is explained by the interaction with different molecules [ 41 ]. In reconstituted lipid bilayers, the amplitude and frequency of ionic oscillations of TRPP2 were strongly dependent on the applied voltage, the Ca 2+ gradient, and the presence of high Ca 2+ [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

TRPP2 is located in the primary cilia of human non-pigmented ciliary epithelial cells

Zheng

Ziemssen

Suesskind

et al. 2023

Graefes Arch Clin Exp Ophthalmol

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Purpose Mechanosensitive channels (MSCs) and primary cilium possess a possible relevance for the sensation of intraocular pressure (IOP). However, there is only limited data on their expression and localization in the ciliary body epithelium (CBE). The purpose of this study was to characterize the expression and localization of TRPP2 in a human non-pigmented ciliary epithelial cell (HNPCE) line. Methods The expression of the TRPP2 was studied by quantitative (q)RT-PCR and in situ hybridization in rat and human tissue. Protein expression and distribution were studied by western blot analysis, immunohistochemistry, and immunoelectron microscopy. Cellular location of TRPP2 was determined in rat and human CBE by immunofluorescence and immunoblot analysis. Electron microscopy studies were conducted to evaluate where and with substructure TRPP2 is localized in the HNPCE cell line. Results The expression of TRPP2 in rat and human non-pigmented ciliary epithelium was detected. TRPP2 was mainly located in nuclei, but also showed a punctate distribution pattern in the cytoplasm of HNPCE of the tissue and the cell line. In HNPCE cell culture, primary cilia did exhibit different length following serum starvation and hydrostatic pressure. TRPP2 was found to be colocalized with these cilia in HNPCE cells. Conclusion The expression of TRPP2 and the primary cilium in the CB may indicate a possible role, such as the sensing of hydrostatic pressure, for the regulation of IOP. Functional studies via patch clamp or pharmacological intervention have yet to clarify the relevance for the physiological situation or aqueous humor regulation.

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Section: Discussionmentioning

confidence: 99%