High Atopobium vaginae and Gardnerella vaginalis Vaginal Loads Are Associated With Preterm Birth

Bretelle, Florence; Rozenberg, Patrick; Pascal, Alain; Favre, R.; Bohec, Caroline; Loundou, Anderson; Sénat, Marie-Victoire; Aïssi, Germain; Lesavre, Nathalie; Brunet, Julie; Heckenroth, H.; Luton, Dominique; Raoult, Didier; Fénollar, Florence; Cocallemen, Jean-François; Henry-Mary, Mireille; Boubli, L.; D’ercole, C.; Chau, C.; Piechon, L.; Zarka, Valérie; Desbrière, Raoul; Shojai, R.; Puppo, Séverine; Franchi, Fabrice; Troyer, Jérémie de; Carcopino, X.; Nakib, Maryam Al; Ménard, Jean-Pierre; Haumonté, Jean-Baptiste; Gamerre, M.; Agostini, Aubert; Capelle, M.; Courbière, Blandine; Mazouni, Chafika; Banet, Julie; Blanc, Karina; Collette, Emmanuelle; Chauveaud-Lambling, Aurélia; Salomon, Laurent; Saada, Lisa; Ducarme, Guillaume; Mougel, Laurence; Vayssière, Christophe; Fort, Julie; Javoise, Sophie

doi:10.1093/cid/ciu966

Cited by 95 publications

(85 citation statements)

References 36 publications

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“…Indeed, G. vaginalis and an increased sialidase activity from vaginal samples are associated with disease and increased risk of PTB (Simhan et al 2003; Cauci et al 2005; Bretelle et al 2015). In addition, symptomatic BV cases tend to be associated sialidase positive readings of swabs (Lopes Dos Santos Santiago et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of sialidase activity and cellular invasion by the bacterial vaginosis pathogen Gardnerella vaginalis

et al. 2018

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Bacterial vaginosis is a genital tract infection, thought to be caused by transformation of a lactobacillus-rich flora to a dysbiotic microbiota enriched in mixed anaerobes. The most prominent of these is Gardnerella vaginalis (GV), an anaerobic pathogen that produces sialidase enzyme to cleave terminal sialic acid residues from human glycans. Notably, high sialidase activity is associated with preterm birth and low birthweight. We explored the potential of the sialidase inhibitor Zanamavir against GV whole cell sialidase activity using methyl–umbelliferyl neuraminic acid (MU-NANA) cleavage assays, with Zanamavir causing a 30% reduction in whole cell GV sialidase activity (p < 0.05). Furthermore, cellular invasion assays using HeLa cervical epithelial cells, infected with GV, demonstrated that Zanamivir elicited a 50% reduction in cell association and invasion (p < 0.05). Our data thus highlight that pharmacological sialidase inhibitors are able to modify BV-associated sialidase activity and influence host–pathogen interactions and may represent novel therapeutic adjuncts.

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Section: Discussionmentioning

confidence: 99%

Inhibition of sialidase activity and cellular invasion by the bacterial vaginosis pathogen Gardnerella vaginalis

et al. 2018

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“…In addition to being an STI, vaginosis has been associated with preterm birth (63). It can be diagnosed at the POC by using ion motility spectrometry (64) and the more widespread ICT and NAAT (63). These assays target mainly two bacteria, Atopobium vaginae and Gardnerella vaginalis.…”

Section: Genital Tract Infectionmentioning

confidence: 99%

The Point-of-Care Laboratory in Clinical Microbiology

et al. 2016

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International audiencePoint-of-care (POC) laboratories that deliver rapid diagnoses of ă infectious diseases were invented to balance the centralization of core ă laboratories. POC laboratories operate 24 h a day and 7 days a week to ă provide diagnoses within 2 h, largely based on immunochromatography and ă real-time PCR tests. In our experience, these tests are conveniently ă combined into syndrome-based kits that facilitate sampling, including ă self-sampling and test operations, as POC laboratories can be operated ă by trained operators who are not necessarily biologists. POC ă laboratories are a way of easily providing clinical microbiology testing ă for populations distant from laboratories in developing and developed ă countries and on ships. Modern Internet connections enable support from ă core laboratories. The cost-effectiveness of POC laboratories has been ă established for the rapid diagnosis of tuberculosis and sexually ă transmitted infections in both developed and developing countries

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“…In pregnant women, this vaginal dysbiosis is the consequence of certain complications such as miscarriage, preterm birth or chorioamnionitis [3]. Bacterial vaginosis is mostly treated with antibiotics, mainly metronidazole and clindamycin, but treatment frequently fails; the relapse rate is estimated at 50% at 6 months [4], [5].…”

Section: Introductionmentioning

confidence: 99%

Taxonogenomics and description of Vaginella massiliensis gen. nov., sp. nov., strain Marseille P2517T, a new bacterial genus isolated from the human vagina

Diop

Bretelle

Michelle

et al. 2017

New Microbes and New Infections

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An obligate aerobic, Gram-negative, nonmotile and nonsporulating rod designated Marseille P2517 was isolated from the vaginal flora. We describe its features, annotate the genome and compare it to the closest species. The 16S rRNA analysis shows 93.03% sequence similarity with Weeksella virosa, the phylogenetically closest species. Its genome is 2 434 475 bp long and presents 38.16% G+C. On the basis of these data, it can be considered as a new genus in the Flavobacteriaceae family, for which we proposed the name Vaginella massiliensis gen. nov., sp. nov. The type strain is Marseille P2517T.

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High Atopobium vaginae and Gardnerella vaginalis Vaginal Loads Are Associated With Preterm Birth

Abstract: NCT00484653.

Cited by 95 publications

References 36 publications

Inhibition of sialidase activity and cellular invasion by the bacterial vaginosis pathogen Gardnerella vaginalis

Inhibition of sialidase activity and cellular invasion by the bacterial vaginosis pathogen Gardnerella vaginalis

The Point-of-Care Laboratory in Clinical Microbiology

Taxonogenomics and description of Vaginella massiliensis gen. nov., sp. nov., strain Marseille P2517T, a new bacterial genus isolated from the human vagina

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