High Antiplasmodial Activity of Golden Gamat (S.hermanni) Extract Through In Vitro Study

Abstract

S.hermanni is a marine biota that contains active components that are anti-fungal, anti-bacterial, antioxidant, anti-inflammatory, and anti-cancer. The research focused on examining the antiplasmodial activity of an ethanol extract of S. hermanni on the proliferation of P. falciparum in vitro. The total sample size was 30 mediums, which included: (G1). No extract or chloroquine administration in the P.falciparum medium (G2). Chloroquine treatment administration on P. falciparum medium (G3). P. falciparum medium with S. hermanni extract. Parasitaemia, growth percentage, inhibitory rate, and IC50 were among the parameters evaluated. Administration of extract serial doses succeeds in reducing the percentage of parasite growth and parasitemia levels. G3 demonstrated an inhibitory rate of 88.51 % with a dose of 100 µg/ml and an IC50 of 2.86 µg/ml, indicating high antimalarial activity, although chloroquine had greater antimalarial activity than S. hermanni extract. In vitro studies on S hermanni, ethanol extract indicated that it contains bioactive components and can be an effective antiplasmodial agent.

Antimalarial activity prediction analysis of sticophus hermanni on plasmodium falciparum hexose transporter (PfHT1)

“…The affinity between receptors and ligands will decrease as binding energy increases. Conversely, higher receptor affinities are associated with lower binding energy values [38,39,40,41,42,43,44,45,46,47,48,49].…”

In Vitro Study: Antimalarial Activity of Rivet Sea Cucumber Extract (Holothuria atra) With Ethyl Acetate Solvent Against Plasmodium falciparum

“…This research was in vitro study with a posttest-only control group design. The experimental group was divided into three groups, of which (1) The negative control group included ten culture media of P. falciparum without antimalarial drugs or H. atra extract; (2) The positive control group included ten culture media of P. falciparum with antimalarial drug administration (chloroquine) but no extract administration; and (3) A treatment group included ten culture media of P. falciparum with ethyl acetate extract H. atra administration, but no antimalarial administration (18) .…”

STUDI IN VITRO: PENGARUH PEMBERIAN EKSTRAK Holothuria athra DENGAN PELARUT N-HEKSANA TERHADAP PERTUMBUHAN Plasmodium falciparum

“…Aktivitas antimalaria dinyatakan tinggi bila nilai IC50 < 5 μg/mL, tergolong menjanjikan bila nilai IC50 < 5 > μg/mL, tergolong sedang bila IC50 < 15-50 μg/mL, dan tidak aktif bila IC50 >50 μg/mL. Mengacu pada standard pengukuran aktivitas zat aktif tersebut, maka ekstrak nheksana H. athra memiliki aktivitas antimlaria yang tinggi (8,13) . (14,15) ; (b) Flavonoid sebagai antimalaria menghambat transport nutrisi dengan pembentukan membran tertentu (New Permeation Pathway/NPP) (14,15) ; (c) Saponin mengandung bahan aktif yang berkarakteristik seperti sabun dan memiliki kemampuan menghemolisis darah (16,17) ; (d) Terpenoid memiliki peran antimalaria / anti hemozoin pada penyakit malaria untuk menghambat mekanisme biokristalisasi heme dari hasil degradasi sel darah merah seperti 4-aminoquinolines dan menghambat pemecahan hemoglobin (18,19) .…”

“…Dari hasil analisis probit penelitian ini menunjukkan IC50 pada ekstrak heksana yaitu sebesar 1.23 μg/ml lebih tinggi dari kontrol positif yaitu klorokuin sebesar 0,4 μg/mL. Hal ini berarti kandungan ekstrak n-heksana Holothuria athra mempunyai potensi sangat aktif sebagai antimalaria (8,13) .…”