High- and low-affinity binding sites for Cd on the bacterial cell walls of Bacillus subtilis and Shewanella oneidensis

Mishra, Bhoopesh; Boyanov, Maxim I.; Bunker, Bruce A.; Kelly, Shelly D.; Kemner, Kenneth M.; Fein, Jeremy B.

doi:10.1016/j.gca.2010.02.019

Cited by 104 publications

(83 citation statements)

References 60 publications

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“…Attempts to include this indicated that large disorder in the path distance reduces the amplitude enough to make it unobservable. Guiné et al (2006) and Mishra et al (2009Mishra et al ( , 2010 studied Zn and Cd adsorption to bacteria and observed Zn and Cd bound to sulfhydryl sites on bacterial surfaces in some samples. No second shell carbon atom in the sulfhydryl group was reported in these studies either, presumably for the same reason.…”

Section: Standardmentioning

confidence: 99%

An X-ray Absorption Fine Structure study of Au adsorbed onto the non-metabolizing cells of two soil bacterial species

Song

Kenney

Fein

et al. 2012

Geochimica et Cosmochimica Acta

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Section: Standardmentioning

confidence: 99%

An X-ray Absorption Fine Structure study of Au adsorbed onto the non-metabolizing cells of two soil bacterial species

Song

Kenney

Fein

et al. 2012

Geochimica et Cosmochimica Acta

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“…solution pH, temperature, interaction with co-ions, etc. (Krauter et al, 1996;Fein et al, 1997;Fein et al, 2001;Borrok et al, 2004;Mishra et al, 2009;Mishra et al, 2010., Tsezos, 1995.…”

Section: Introductionmentioning

confidence: 99%

Adsorption behaviour of cadmium byBacillus cereusM¹₁₆: some physical and biochemical studies

Ganguly

Guha

Ray

2011

Chemical Speciation & Bioavailability

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Adsorption behaviour of cadmium from its aqueous solution by growing and non-growing cells of a mutant strain of Bacillus cereus M 1 16 has been studied to explore the possibility of the biomass to address environmental pollution due to this toxic metal. The results establish that about 75% and 88% of the cadmium can be removed by growing and non-growing cells of the selected strain respectively from its aqueous solution at pH 6.8 AE 0.2, temperature 30 AE 1 C and 120 rpm shaking speed. For growing cells, inoculum size 2% and medium volume 50 mL is found to be optimum. The adsorption rate of cadmium on the biomass is very fast initially and attains equilibrium within 60 min following pseudo second-order rate model (R 2 ¼ 0:99). The equilibrium adsorption isotherm can be best described by Langmuir -Freundlich dual model (R 2 ¼ 0:99) indicating that both physisorption and chemisorption take place simultaneously. Cadmium can be desorbed from the loaded biomass using mineral acids (0.1 M).

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“…Cd (Guiné et al 2006;Mishra et al, 2010;Yu and Fein, 2015). Association of Hg with reduced 519 S groups has also been shown in phytoplankton (diatoms) collected from a Hg contaminated 520 creek using a combination of x-ray micro-fluorescence mapping and FTIR studies (Gu et al, 521 2014).…”

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confidence: 99%

Stoichiometry of mercury-thiol complexes on bacterial cell envelopes

Mishra

Shoenfelt

et al. 2017

Chemical Geology

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30We have examined the speciation of Hg(II) complexed with intact cell suspensions (10 13 31 cells L -1 ) of Bacillus subtilis, a common gram-positive soil bacterium, Shewanella oneidensis 32 MR-1, a facultative gram-negative aquatic organism, and Geobacter sulfurreducens, a gram-33 negative anaerobic bacterium capable of Hg-methylation at Hg(II) loadings spanning four orders 34 of magnitude (120 nM to 350 µM) at pH 5.5 (±0.2). The coordination environments of Hg on 35 bacterial cells were analyzed using synchrotron based X-ray Absorption Near Edge Structure 36 (XANES) and Extended X-ray Absorption Fine Structure (EXAFS) spectroscopy at the Hg LIII 37 edge. The abundance of thiols on intact cells was determined by a fluorescence-spectroscopy 38 based method using a soluble bromobimane, monobromo(trimethylammonio)bimane (qBBr) to 39 block thiol sites, and potentiometric titrations of biomass with and without qBBr treatment. The 40 chemical forms of S on intact bacterial cells were determined using S k-edge XANES 41 spectroscopy. 42Hg(II) was found to complex entirely with cell bound thiols at low Hg:biomass ratios. 43 For Bacillus subtilis and Shewanella oneidensis MR-1 cells, the Hg-S stoichiometry changed 44 from Hg-S3 to Hg-S2 and Hg-S (where 'S' represents a thiol site such as is present on cysteine) 45 progressively as the Hg(II) loading increased on the cells. However, Geobacter sulfurreducens 46 did not form Hg-S3 complexes. Because the abundance of thiol was highest for Geobacter 47 sulfurreducens (75 µM/g wet weight) followed by Shewanella oneidensis MR-1 (50 µM/g wet 48 weight) and Bacillus subtilis (25 µM/g wet weight), the inability of Hg(II) to form Hg-S3 49 complexes on Geobacter sulfurreducens suggests that the density and reactivity of S-amino acid 50 containing cell membrane proteins on Geobacter sulfurreducens are different from those of 51Bacillus subtilis and Shewanella oneidensis MR-1. Upon saturation of the high affinity thiol sites 52 at higher Hg:biomass ratios, Hg(II) was found to form a chelate with -hydroxy carboxylate 53anion. The stoichiometry of cell envelope bound Hg-thiol complexes and the associated 54 abundance of thiols on the cell envelopes provide important insights for understanding the 55 differences in the rate and extent of uptake and redox transformations of Hg in the environment. Introduction 66Mercury is a common contaminant found in many terrestrial and aquatic systems, and its 67 bioaccumulation in organisms, including humans, is a major environmental concern (Mergler et 68 al, 2007). The solubility, speciation, toxicity and the ultimate fate of Hg within aquatic 69 ecosystems is dependent on a large number of chemical and biological variables (Morel et al., 70 1998;Barkay and Schaefer, 2001). In aquatic systems, Hg solubility is high under oxygen-rich 71 acidic conditions but it is significantly inhibited under anoxic sulfide-rich waters (Martell and 72 Smith, 1974). The Hg-sulfide complexes are among the strongest complexes of all known Hg 73 i...

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High- and low-affinity binding sites for Cd on the bacterial cell walls of Bacillus subtilis and Shewanella oneidensis

Cited by 104 publications

References 60 publications

An X-ray Absorption Fine Structure study of Au adsorbed onto the non-metabolizing cells of two soil bacterial species

An X-ray Absorption Fine Structure study of Au adsorbed onto the non-metabolizing cells of two soil bacterial species

Adsorption behaviour of cadmium byBacillus cereusM¹₁₆: some physical and biochemical studies

Stoichiometry of mercury-thiol complexes on bacterial cell envelopes

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High- and low-affinity binding sites for Cd on the bacterial cell walls of Bacillus subtilis and Shewanella oneidensis

Cited by 104 publications

References 60 publications

An X-ray Absorption Fine Structure study of Au adsorbed onto the non-metabolizing cells of two soil bacterial species

An X-ray Absorption Fine Structure study of Au adsorbed onto the non-metabolizing cells of two soil bacterial species

Adsorption behaviour of cadmium byBacillus cereusM116: some physical and biochemical studies

Stoichiometry of mercury-thiol complexes on bacterial cell envelopes

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Adsorption behaviour of cadmium byBacillus cereusM¹₁₆: some physical and biochemical studies