High Allelic Diversity of Dog Leukocyte Antigen Class II in East Asian Dogs: Identification of New Alleles and Haplotypes

Kang, Mingue; Ahn, Byeongyong; Youk, Seungyeon; Cho, Hyesun; Choi, Min‐Koo; Hong, Kwonho; Tae, Jeong; Song, Hyuk; Jiang, Hao; Kennedy, L. J.; Park, Chankyu

doi:10.1007/s10914-021-09560-x

Cited by 3 publications

(1 citation statement)

References 52 publications

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“…Sapsarees, a native dog breed, have existed in the Korean peninsula for at least a few hundred years and carry an identical allele responsible for the hair phenotype of the European dog breeds [ 35 ] despite distant genetic relationships between Sapsarees and Western dog breeds [ 36 , 37 , 38 ]. Currently, 197 long-haired dog breeds have been officially recognized by the American Kennel Club (AKC, ; accessed on 26 September 2021), and their hair length variations have been explained by the FGF5 and RSPO2 variations [ 11 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Tracing the Origin of the RSPO2 Long-Hair Allele and Epistatic Interaction between FGF5 and RSPO2 in Sapsaree Dog

Kang

Ahn

Youk

et al. 2022

Genes

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Genetic analysis of the hair-length of Sapsaree dogs, a Korean native dog breed, showed a dominant mode of inheritance for long hair. Genome-Wide Association Study (GWAS) analysis and subsequent Mendelian segregation analysis revealed an association between OXR1, RSPO2, and PKHD1L1 on chromosome 13 (CFA13). We identified the previously reported 167 bp insertion in RSPO2 3’ untranslated region as a causative mutation for hair length variations. The analysis of 118 dog breeds and wolves revealed the selection signature on CFA13 in long-haired breeds. Haplotype analysis showed the association of only a few specific haplotypes to the breeds carrying the 167 bp insertion. The genetic diversity in the neighboring region linked to the insertion was higher in Sapsarees than in other Asian and European dog breeds carrying the same variation, suggesting an older history of its insertion in the Sapsaree genome than in that of the other breeds analyzed in this study. Our results show that the RSPO2 3’ UTR insertion is responsible for not only the furnishing phenotype but also determining the hair length of the entire body depending on the genetic background, suggesting an epistatic interaction between FGF5 and RSPO2 influencing the hair-length phenotype in dogs.

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Section: Discussionmentioning

confidence: 99%

Tracing the Origin of the RSPO2 Long-Hair Allele and Epistatic Interaction between FGF5 and RSPO2 in Sapsaree Dog

Kang

Ahn

Youk

et al. 2022

Genes

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Characterization of the polymorphisms of dog MHC genes and intra- and interbreed DLA haplotype diversity

Miyamae,

Okano,

Katakura

et al. 2024

MHC

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The domestic dog (Canis lupus familiaris) is one of the main target animals for small animal clinical medicine Consolidation of the polymorphism information of Dog MHC (Dog leukocyte antigen: DLA) genes is necessary for discovering disease-associations and developing advanced veterinary medicine of dogs such as regenerative medicine by allo-transplantation of somatic or hematopoietic stem cells and cancer immunotherapy We have so far conducted polymorphism analysis of the DLA genes in 829 dogs of 59 breeds collected in Japan In this review, we outlined the current information of the DLA polymorphisms including our research findings such as haplotype structures of the DLA class I loci, estimation of the DLA haplotypes composed of the DLA class I and class II loci, and characteristics of intra-and inter-breed DLA diversity

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Large-Scale Polymorphism Analysis of Dog Leukocyte Antigen Class I and Class II Genes (DLA-88, DLA-12/88L and DLA-DRB1) and Comparison of the Haplotype Diversity between Breeds in Japan

Miyamae

Okano

Katakura

et al. 2023

Cells

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Polymorphisms of canine leukocyte antigen (DLA) class I (DLA-88 and DLA-12/88L) and class II (DLA-DRB1) genes are important for disease susceptibility studies, but information on the genetic diversity among dog breeds is still lacking. To better elucidate the polymorphism and genetic diversity between breeds, we genotyped DLA-88, DLA-12/88L, and DLA-DRB1 loci using 829 dogs of 59 breeds in Japan. Genotyping by Sanger sequencing identified 89, 43, and 61 alleles in DLA-88, DLA-12/88L, and DLA-DRB1 loci, respectively, and a total of 131 DLA-88–DLA-12/88L–DLA-DRB1 haplotypes (88-12/88L-DRB1) were detected more than once. Of the 829 dogs, 198 were homozygotes for one of the 52 different 88-12/88L-DRB1 haplotypes (homozygosity rate: 23.8%). Statistical modeling suggests that 90% of the DLA homozygotes or heterozygotes with one or other of the 52 different 88-12/88L-DRB1 haplotypes within somatic stem cell lines would benefit graft outcome after 88-12/88L-DRB1-matched transplantation. As previously reported for DLA class II haplotypes, the diversity of 88-12/88L-DRB1 haplotypes varied remarkably between breeds but was relatively conserved within most breeds. Therefore, the genetic characteristics of high DLA homozygosity rate and poor DLA diversity within a breed are useful for transplantation therapy, but they may affect biological fitness as homozygosity progresses.

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High Allelic Diversity of Dog Leukocyte Antigen Class II in East Asian Dogs: Identification of New Alleles and Haplotypes

Cited by 3 publications

References 52 publications

Tracing the Origin of the RSPO2 Long-Hair Allele and Epistatic Interaction between FGF5 and RSPO2 in Sapsaree Dog

Tracing the Origin of the RSPO2 Long-Hair Allele and Epistatic Interaction between FGF5 and RSPO2 in Sapsaree Dog

Characterization of the polymorphisms of dog MHC genes and intra- and interbreed DLA haplotype diversity

Large-Scale Polymorphism Analysis of Dog Leukocyte Antigen Class I and Class II Genes (DLA-88, DLA-12/88L and DLA-DRB1) and Comparison of the Haplotype Diversity between Breeds in Japan

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