High-affinity laminin receptor is a receptor for Sindbis virus in mammalian cells

Wang, Kang-Sheng; Kühn, Richard; Strauss, Ellen G.; Ou, Susan; Strauss, James H.

doi:10.1128/jvi.66.8.4992-5001.1992

Cited by 299 publications

(121 citation statements)

References 54 publications

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“…These membrane proteins were low-density lipoprotein receptor (LDLr), LamR, and vinculin. Among these, LamR has been identified to be a cellular receptor for different pathogens (38)(39)(40)(41)(42)(43)(44)(45)(46)(47). As shown in Fig.…”

Section: Membrane Proteins Required For Csfv Infection Were Screened mentioning

confidence: 99%

The Laminin Receptor Is a Cellular Attachment Receptor for Classical Swine Fever Virus

Chen

Shen

et al. 2015

J Virol

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Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious, economically important viral disease in many countries. The E rns and E2 envelope glycoproteins are responsible for the binding to and entry into the host cell by CSFV. To date, only one cellular receptor, heparan sulfate (HS), has been identified as being involved in CSFV attachment. HS is also present on the surface of various cells that are nonpermissive to CSFV. Hence, there must be another receptor(s) that has been unidentified to date. In this study, we used a set of small interfering RNAs (siRNAs) against a number of porcine cell membrane protein genes to screen cellular proteins involved in CSFV infection. This approach resulted in the identification of several proteins, and of these, the laminin receptor (LamR) has been demonstrated to be a cellular receptor for several viruses. Confocal analysis showed that LamR is colocalized with CSFV virions on the membrane, and a coimmunoprecipitation assay indicated that LamR interacts with the CSFV E rns protein. In inhibition assays, anti-LamR antibodies, soluble laminin, or LamR protein significantly inhibited CSFV infection in a dose-dependent manner. Transduction of PK-15 cells with a recombinant lentivirus expressing LamR yielded higher viral titers. Moreover, an attachment assay demonstrated that LamR functions during virus attachment. We also demonstrate that LamR acts as an alternative attachment receptor, especially in SK6 cells. These results indicate that LamR is a cellular attachment receptor for CSFV. IMPORTANCE Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), an economically important viral disease affecting the pig industry in many countries. To date, only heparan sulfate (HS) has been identified to be an attachment receptor for CSFV. Here, using RNA interference screening with small interfering RNAs (siRNAs) against a number of porcine membrane protein genes, we identified the laminin receptor (LamR) to be another attachment receptor. We demonstrate the involvement of LamR together with HS in virus attachment, and we elucidate the relationship between LamR and HS. LamR also serves as an attachment receptor for many viral pathogens, including dengue virus, a fatal human flavivirus. The study will help to enhance our understanding of the life cycle of flaviviruses and the development of antiviral strategies for flaviviruses. C lassical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious and often fatal viral disease in pigs. The disease leads to significant economic losses in many countries. CSFV is a member of the Pestivirus genus within the Flaviviridae family (1). The virus possesses a singlestranded, positive-sense RNA genome of approximately 12.3 kb (2, 3). Its genome contains a single, large open reading frame that encodes a precursor polyprotein of 3,898 amino acids (aa). The polyprotein is co-and posttranslationally processed by viral an...

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Section: Membrane Proteins Required For Csfv Infection Were Screened mentioning

confidence: 99%

The Laminin Receptor Is a Cellular Attachment Receptor for Classical Swine Fever Virus

Chen

Shen

et al. 2015

J Virol

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“…The high-affinity laminin receptor [108] and heparan sulfate [109] have been reported to enhance, but not to be essential for alphavirus infection into certain cell types. In the past year, Cherry and collaborators identified the 'Natural Resistance-Associated Macrophage Protein' (NRAMP) as an SV entry receptor [110 ].…”

Section: Nramp Is An Sv Entry Receptormentioning

confidence: 99%

Virus entry: old viruses, new receptors

Backović

Rey

2012

Current Opinion in Virology

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The long-sought entry receptors for rubella, sindbis and respiratory syncytial viruses (RV, SV and RSV), together with the missing measles virus (MV) receptor for infection of epithelial cells, were identified in 2011. These have been major developments in the field of virus entry. In addition, 2011 was rich in new information about the interactions of MV, RSV and phleboviruses with DC-SIGN during infection of dendritic cells, a crucial step allowing the virus to breach the epithelial barrier and gain access to the lymph nodes. This faciliates dissemination to susceptible tissues where it can develop a vigorous and sustained replication, to eventually target specific organs from which it can propagate into the environment and efficiently infect new hosts, closing the merry-go-round of the virus cycle.

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“…High-affinity laminin receptor (Wang et al, 1992); HS-GAG (Byrnes and Griffin, 1998;Klimstra et al, 1998)…”

Section: Sindbis Virusmentioning

confidence: 99%

Evolution of Cell Recognition by Viruses: A Source of Biological Novelty with Medical Implications

Baranowski

Ruiz-Jarabo

Pariente

et al. 2003

Advances in Virus Research

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Receptor classCellular structure a Extracellular matrix components, sugar derivatives and lipids GalactosylceramideGangliosides Glycosaminoglycans (heparan and chondroitin sulfates) Phospholipids Sialic acid (N-acetylneuraminic acid, N-acetyl-9-O-acetylneuraminic acid and N-glycolylneuraminic acid 3-O-sulfated heparan sulfate Cell adhesion and cell-cell contact proteins -Dystroglycan Coxsackievirus-adenovirus receptor (CAR; Ig superfamily) CD4 (Ig superfamily) CEACAMs (including Bgp1a, Bgp2, and pregnancy-specific glycoprotein) Intercellular adhesion molecule type 1 (ICAM-1; Ig superfamily) Integrins Junction adhesion molecule (JAM) Laminin receptor (high affinity) MHC class I and 2 -microglobulin Neural cell adhesion molecule (NCAM) Signaling lymphocyte activation molecule

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High-affinity laminin receptor is a receptor for Sindbis virus in mammalian cells

Cited by 299 publications

References 54 publications

The Laminin Receptor Is a Cellular Attachment Receptor for Classical Swine Fever Virus

The Laminin Receptor Is a Cellular Attachment Receptor for Classical Swine Fever Virus

Virus entry: old viruses, new receptors

Evolution of Cell Recognition by Viruses: A Source of Biological Novelty with Medical Implications

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