High-affinity incorporation of 20-carbon polyunsaturated fatty acids by human skin fibroblasts

Banerjee, Nandita; Rosenthal, Miriam D.

doi:10.1016/0005-2760(85)90122-5

Cited by 18 publications

(9 citation statements)

References 19 publications

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“…One possible mechanism is that n-3 PUFA may compete effectively with newly synthesized AA and reduce its acylation into liver lipids. Such competition between fatty acids has been documented previously in in vitro studies (21)(22)(23)(24). A second possible mechanism is that one or more of the n-3 PUFA in FO inhibits the conversion of LA to AA.…”

Section: Discussionsupporting

confidence: 69%

Dietary fish oil inhibits Δ6‐desaturase activity in vivo

Raz

Kamin-Belsky

Przedecki

et al. 1998

J Americ Oil Chem Soc

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Liver Δ6‐desaturase activity was determined in mice which were made deficient in (i) n‐6 and n‐3 polyunsaturated fatty acids (PUFA), (ii) n‐6 PUFA, or (iii) arachidonic acid (AA). Initially, the mice were subjected to two cycles of a fasting (1 d)/refeeding (2–3 d) protocol in which they were refed an essential fatty acid‐deficient (EFAD) diet during the refeeding period. This 1‐wk fasting/refeeding protocol, referred to as F/R EFAD, produced a rapid and substantial decline in tissue n‐3 and n‐6 PUFA and a corresponding increase in n‐9 fatty acids, notably oleic acid and Mead acid (20:3n‐9). Combined liver Δ6‐desaturase/elongase/Δ5‐desaturase activities in vivo were quantified by measuring the conversion of 14C‐linoleic acid (LA) to 14C‐AA in mouse liver. Although F/R EFAD caused, as expected, a substantial decline in liver AA and LA content, the conversion of 14C‐LA to 14C‐AA was the same in these mice as in chow‐fed controls (approximately 33–34%). Subsequent refeeding of F/R EFAD mice with an EFAD diet, supplemented with corn oil, restored tissue n‐6 PUFA levels without altering the conversion of 14C‐LA to 14C‐AA. In contrast, refeeding with an EFAD diet, supplemented with fish oil, inhibited 14C‐LA to 14C‐AA conversion by 78%. Significantly, inhibition of conversion of 14C‐LA to 14C‐AA was maintained in F/R EFAD mice that were subsequently fed an EFAD diet supplemented with a 1:1 mixture of fish oil/corn oil. This latter protocol yielded a unique liver fatty acid composition in which AA was selectively depleted, whereas LA and the n‐3 PUFA were increased. The data suggest that dietary n‐3 C20–22 PUFA negatively regulate the in vivo synthesis of n‐6 PUFA at the level of the Δ6‐desaturase.

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Section: Discussionsupporting

confidence: 69%

Dietary fish oil inhibits Δ6‐desaturase activity in vivo

Raz

Kamin-Belsky

Przedecki

et al. 1998

J Americ Oil Chem Soc

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“…Eicosapentaenoic acid (EPA; 20:5n-3) is a potential candidate as a competitor for the incorporation and metabolism of AA in tick salivary glands and studies addressing this issue were performed in vivo and in vitro in the lone star tick, Amblyomma americanum. The uptake of 13HlAA by cultured human skin fibroblasts was unaffected by the presence of 5 pM oleate or linoleate, but was reduced one-third by the presence of 5 pM AA or EPA (Banerjee and Rosenthal, 1985). In the present study, t3H1AA uptake by isolated tick salivary glands was unaffected by 5 pM oleate and linoleate, but reduced by 5 pM AA and EPA, though to a lesser extent.…”

Section: Discussioncontrasting

confidence: 44%

“…Increased levels of exogenous AA resulted in an increase of [3H]arachidonate in the TG fraction and a concomitant decrease in the PL fraction in isolated salivary glands . Similar effects of high media concentrations of AA on increased arachidonyl-triglycerides were reported for human skin fibroblasts (Banerjee and Rosenthal, 1985) and promyelocytic leukemia cells (Blank et al, 1992). It was suggested that the tick salivary gland contains a saturable LAT activity which allows the diacylglycerol acyltransferase (DAT, E.C.…”

Section: Introductionmentioning

confidence: 71%

Alteration of arachidonate levels in tick salivary glands by dietary modification of host blood lipids

Madden

Sauer

Dillwith

et al. 1996

Arch. Insect Biochem. Physiol.

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Tick saliva contains prostaglandins of the 2‐series, believed to facilitate bloodmeal acquisition. Because ticks cannot synthesize the prostaglandin precursor, arachidonic acid, investigations were undertaken to study the uptake, incorporation, and distribution of arachidonic acid in the salivary glands of the lone star tick in vitro and in vivo. Uptake of [3H]arachidonate by isolated salivary glands was reduced in the presence of low concentrations of arachidonic or eicosapentaenoic acids, but much higher, non‐physiological concentrations of oleic and linoleic acids were required to inhibit [3H]arachidonate uptake. The incorporation of [3H]arachidonate into triglycerides increased at high concentrations of arachidonic or eicosapentaenoic acid, but not at any concentration of oleic or linoleic acid. Eicosatetraynoic acid greatly inhibited [3H]arachidonate uptake and increased intracellular unesterified [3H]arachidonic acid. Guinea pigs fed hydrogenated coconut oil, safflower/primrose oil, or fish oil exhibited altered blood lipids; notably increased levels of eicosapentaenoic acid when fed fish oil. Salivary gland lipids in ticks fed on these hosts were also altered. Ticks parasitizing fish oil‐fed guinea pigs contained high levels of eicosapentaenoic acid with a 30% reduction in arachidonate levels. The results demonstrated that eicosapentaenoic acid in the host diet had profound effects on arachidonate assimilation by tick salivary glands, which could lead to altered prostaglandin content in tick saliva. © 1996 Wiley‐Liss, Inc.

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“…Because dietary AA does not have to be desaturated and elongated before tissue incorporation, and because it is a poor substrate for β-oxidation, AA can be rapidly and preferentially enriched in membrane phospholipids (1,2). AA is released from membrane lipids by phospholipases and metabolized into a large group of eicosanoids (prostaglandin group 2) including prostaglandin, thromboxane, leukotriene, and prostacyclin, which are important for intercellular signaling at various stages of growth and development (3).…”

mentioning

confidence: 99%

Production of arachidonic acid by filamentous fungus, Mortierella alliacea strain YN‐15

Aki

Nagahata

Ishihara

et al. 2001

J Americ Oil Chem Soc

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A filamentous fungus producing significant levels of arachidonic acid (AA, C20:4n-6) was isolated from a freshwater pond sample and assigned to the species Mortierella alliacea. This strain, YN-15, accumulated AA mainly in the form of triglyceride in its mycelia. An optimized culture in 25 L of medium containing 12% glucose and 3% yeast extract yielded 46.1 g/L dry cell weight, 19.5 g/L total fatty acid, and 7.1 g/L AA by 7-d cultivation in a 50-L jar fermenter. Assimilation of soluble starch by YN-15 was notably enhanced by the addition of oleic acid, soybean oil, ammonium sulfate, or potassium phosphate to a starch-based medium. Using starch as a main carbon source in the pre-pilot scale cultivation improved the production of AA by up to 5.0 g/L. Mortierella alliacea strain YN-15 is therefore a promising fungal isolate for industrial production of AA and other polyunsaturated fatty acids.Paper no. J9821 in JAOCS 78, 599-604 (June 2001).

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High-affinity incorporation of 20-carbon polyunsaturated fatty acids by human skin fibroblasts

Cited by 18 publications

References 19 publications

Dietary fish oil inhibits Δ6‐desaturase activity in vivo

Dietary fish oil inhibits Δ6‐desaturase activity in vivo

Alteration of arachidonate levels in tick salivary glands by dietary modification of host blood lipids

Production of arachidonic acid by filamentous fungus, Mortierella alliacea strain YN‐15

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