High abundance of heterotrophic prokaryotes in hydrothermal springs of the Azores as revealed by a network of 16S rRNA gene-based methods

Sahm, Kerstin; John, P. Grotzinger; Nacke, Heiko; Wemheuer, Bernd; Grote, Ralf; Daniel, Rolf; Antranikian, Garabed

doi:10.1007/s00792-013-0548-2

Cited by 59 publications

(38 citation statements)

References 52 publications

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“…Although a single hypervariable region is not sufficient to phylogenetically classify microorganisms, the hypervariable regions V2, V3 and V6 show the maximal heterogeneity among the different lineages providing the best discriminating power for the analysis of microbial communities (Chakravorty et al 2007). Today, NGS can produce large 16S rRNA datasets containing hundreds of thousands of 16S RNAs fragments allowing the survey of several microbial communities simultaneously in different hyperthermophilic environments (Hou et al 2013;Sahm et al 2013). Despite the ease with which the 16S rRNA profiling can be made, this approach is known to Proteobacteria (Lysobacter enzymogenes) (Brito et al 2014) N.R.…”

Section: Nr Low Dissolved Oxygen Concentrationmentioning

confidence: 99%

“…Recently, nine sites showing a wide range of physico-chemical characteristics (51-92°C; pH 2.5-8.0) were explored, i.e. AI-AIV near Caldeira Do Esgucho, BI-BIII near Caldeirão and CI and CII near Caldeira Asmodeu (Sahm et al 2013). In this study several approaches were used to assess the prokaryotic diversity, i.e.…”

Section: Furnas Valley Azoresmentioning

confidence: 99%

“…By contrast, in the AIV site (92°C, pH 8.0) an even distribution of archaea (35%) and bacteria (40%) was detected. In particular, phyla Thermotogales (genus Fervidobacterium), Firmicutes (genus Caldicellulosiruptor) and Dictyoglomi (genus Dictyoglomus) were abundant among bacteria, whereas the archaeal population was almost exclusively composed by Crenarchaeota belonging to the Desulfurococcaceae and Thermoproteaceae families (Sahm et al 2013). A general observations was that, once again, the pH was the predominant parameter, influencing microbial complexity in different areas surveyed, with the highest bacterial diversity detected at sites where temperatures and pHs ranged 55-85°C and 7.0-8.0, respectively.…”

Section: Furnas Valley Azoresmentioning

confidence: 99%

“…Intriguingly, unlike other hot spring-environments where Aquificales are dominant, here heterotrophic bacteria prevail. To explain this, authors suggested that a 20-400-fold higher DOC in the Furnas spring could be a reason for the abundance of heterotrophic bacteria (Sahm et al 2013). …”

Section: Furnas Valley Azoresmentioning

confidence: 99%

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Metagenomics of microbial and viral life in terrestrial geothermal environments

Strazzulli

Fusco

Cobucci‐Ponzano

et al. 2017

Rev Environ Sci Biotechnol

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Geothermally heated regions of Earth, such as terrestrial volcanic areas (fumaroles, hot springs, and geysers) and deep-sea hydrothermal vents, represent a variety of different environments populated by extremophilic archaeal and bacterial microorganisms. Since most of these microbes thriving in such harsh biotopes, they are often recalcitrant to cultivation; therefore, ecological, physiological and phylogenetic studies of these microbial populations have been hampered for a long time. More recently, culture-independent methodologies coupled with the fast development of next generation sequencing technologies as well as with the continuous advances in computational biology, have allowed the production of large amounts of metagenomic data. Specifically, these approaches have assessed the phylogenetic composition and functional potential of microbial consortia thriving within these habitats, shedding light on how extreme physico-chemical conditions and biological interactions have shaped such microbial communities. Metagenomics allowed to better understand that the exposure to an extreme range of selective pressures in such severe environments, accounts for genomic flexibility and metabolic versatility of microbial and viral communities, and makes extreme-and hyper-thermophiles suitable for bioprospecting purposes, representing an interesting source for novel thermostable proteins that can be potentially used in several industrial processes.Keywords Hyperthermophiles Á Geothermal environments Á Microbial and viral metagenomics Á CRISPR Á Enzyme discovery BackgroundHot springs populated by extreme thermophiles (T opt : 65-79°C) and hyperthermophiles (T opt [ 80°C) (DeCastro et al. 2016) are very diverse and some of them show combinations of extreme chemo-physical conditions, such as temperature, acidic or alkaline pHs, high pressure, and high concentrations of salts and heavy metals (López-López et al. 2013). As with all studies of environmental microbiology, our understanding of the composition, functional and physiological dynamics, and evolution of extreme-and hyper-thermophilic microbial consortia has lagged A. Strazzulli Á M. Moracci Á P. Contursi

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Section: Nr Low Dissolved Oxygen Concentrationmentioning

confidence: 99%

Section: Furnas Valley Azoresmentioning

confidence: 99%

Section: Furnas Valley Azoresmentioning

confidence: 99%

Section: Furnas Valley Azoresmentioning

confidence: 99%

See 2 more Smart Citations

Metagenomics of microbial and viral life in terrestrial geothermal environments

Strazzulli

Fusco

Cobucci‐Ponzano

et al. 2017

Rev Environ Sci Biotechnol

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“…The majority of previous studies have mainly focused on bacteria and archaea in hot springs (Pan et al 2010;Sahm et al 2013;Hamamura et al 2013). In contrast, the study of fungi has been insufficiently examined.…”

Section: Introductionmentioning

confidence: 99%

Diversity of fungi associated with plants growing in geothermal ecosystems and evaluation of their capacities to enhance thermotolerance of host plants

Zhou

White

Soares

et al. 2015

Journal of Plant Interactions

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Diversity and heat-adaptation of endophytic fungi (EF) and rhizospheric fungi associated with plants growing in geothermal ecosystems, Southwest China, as well as their benefit in improving host plant thermotolerances were investigated. A total of 1589 culturable fungi belonging to 38 taxa were isolated, in which Curvularia, Acrophialophora, Penicillium, and Aspergillus were the dominant genera. The Shannon indices of EF and rhizospheric fungi ranged from 1.80 to 2.56 and 0.73 to 2.11, respectively. Phylogenetic analysis indicated that the EF have a close relationship with rhizospheric fungi. However, some fungi exhibited apparently species-specific habitat distribution patterns. Growth temperature tests indicated that 60.22% of the tested isolates were thermotolerant fungi and only 39.78% were mesophiles, and the number of heat-adapted fungi increased with increasing environmental temperatures. The strain G1-29, which was isolated from the roots of Hedyotis diffusa and identified as Curvularia crepinii, significantly improved host plant thermotolerance under laboratory conditions: the death rate of endophyte-infected plants was significantly lower than that of endophyte-free plants (t-test, p = .0158, df = 4). Our results suggested that the EF and rhizospheric fungi associated with plants growing in geothermal ecosystems are diverse, and many of them have adapted to the high environmental temperatures. Some fungi have come to be the dominant endemic inhabitants of specific niches, and some played an important role in improving host plant thermotolerances.

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