High abundance of Amphidomataceae (Dinophyceae) during the 2015 spring bloom of the Argentinean Shelf and a new, non-toxigenic ribotype of Azadinium spinosum

Tillmann, Urban; Gottschling, Marc; Krock, Bernd; Smith, Kirsty F.; Guinder, Valeria A.

doi:10.1016/j.hal.2019.01.008

Cited by 21 publications

(33 citation statements)

References 63 publications

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“…Both data sets were significantly correlated and allowed the calculation of the field population AZA cell quota. With a mean AZA-1 cell quota of 17.8 fg cell -1 (Fig 9) the field measurements are in the same range as laboratory measurements of isolated strains, which typically vary between~1 and 20 fg cell -1 [11,15,36] and were higher (up to 200 fg cell -1 ) when grown at 10˚C [66]. The range of AZA cell quota as estimated here (e.g.…”

Section: Plos Onesupporting

confidence: 70%

“…poporum, to check whether the assays are still species-specific. Cell isolates of the respective species/strains were cultured and collected as described in Tillmann et al [36]. For each sample, the DNA was extracted from cell pellets according to the procedures for the field samples.…”

Section: Sample Processingmentioning

confidence: 99%

“…Assigned as Ribotype A, these strains are clearly delimited from Norwegian strains with a toxin profile of mainly AZA-11 and -51, which form a different ribotype, Ribotype B [35]. Recently, Tillmann et al [36] found evidence for a third ribotype (Ribotype C), consisting of Az. spinosum strains collected at the Argentinean Shelf in 2015, where no AZA were detected.…”

Section: Introductionmentioning

confidence: 99%

“…languida), and Smith et al [33] designed a SYBR Green assay targeting the DNA of all amphidomatacean species. These qPCR assays have been used to detect Amphidomataceae in field samples, for example on research surveys along the Danish coastline [34], the Norwegian coast [35], the Irish coastline [32], the Argentine shelf [36] and in western Greenland [22], or are in use for the regular monitoring of Az. spinosum and Az.…”

Section: Introductionmentioning

confidence: 99%

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Distribution and abundance of azaspiracid-producing dinophyte species and their toxins in North Atlantic and North Sea waters in summer 2018

et al. 2020

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Representatives of the marine dinophyte family Amphidomataceae produce lipophilic phycotoxins called azaspiracids (AZA) which may cause azaspiracid shellfish poisoning (AZP) in humans after consumption of contaminated seafood. Three of the four known toxigenic species are observed frequently in the eastern North Atlantic. In 2018, a research survey was performed to strengthen knowledge on the distribution and abundance of toxigenic Amphidomataceae and their respective toxins in Irish coastal waters and in the North Sea. Species-specific quantification of the three toxigenic species (Azadinium spinosum, Azadinium poporum and Amphidoma languida) was based on recently developed qPCR assays, whose performance was successfully validated and tested with specificity tests and spike experiments. The multi-method approach of on-board live microscopy, qPCR assays and chemical AZA-analysis revealed the presence of Amphidomataceae in the North Atlantic including the three targeted toxigenic species and their respective AZA analogues (AZA-1,-2,-33,-38,-39). Azadinium spinosum was detected at the majority of Irish stations with a peak density of 8.3 x 10 4 cells L-1 and AZA (AZA-1,-2,-33) abundances up to 1,274 pg L-1. Amphidoma languida was also present at most Irish stations but appeared in highest abundance in a bloom at a central North Sea station with a density of 1.2 x 10 5 cells L-1 and an AZA (AZA-38,-39) abundances of 618 pg L-1. Azadinium poporum was detected sporadically at the Irish south coast and North Sea and was rather low in abundance during this study. The results confirmed the wide distribution and frequent occurrence of the target species in the North Atlantic area and revealed, for the first time, bloom abundances of toxigenic Amphidomataceae in this area. This emphasizes the importance of future studies and monitoring of amphidomatacean species and their respective AZA analogues in the North Atlantic.

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Section: Plos Onesupporting

confidence: 70%

Section: Sample Processingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distribution and abundance of azaspiracid-producing dinophyte species and their toxins in North Atlantic and North Sea waters in summer 2018

et al. 2020

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“…2018a) or the Argentinean shelf (Tillmann & Akselman 2016; Tillmann 2018; Tillmann et al . 2019), and thus seem to be the rule and not an exception.…”

Section: Discussionmentioning

confidence: 93%

Morphological and molecular characterization of multiple new Azadinium strains revealed a high diversity of non‐toxigenic species of Amphidomataceae (Dinophyceae) including two new Azadinium species in Irish waters, North East Atlantic

Salas

Tillmann

et al. 2021

Phycological Research

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SUMMARY Shellfish contamination with azaspiracids (AZA), which are lipophilic marine biotoxins produced by marine dinoflagellates, is a major and recurrent problem for the Irish shellfish industry. AZA are produced by certain species of Amphidomataceae, but the species diversity of this group of microalgae in Irish waters is poorly known. Here we present a morphological and molecular characterization of multiple new strains of non‐toxigenic Azadinium isolated on an oceanographic survey in 2018. A lack of AZA production for all strains presented here was demonstrated by LC‐MS/MS analysis. One strain of Azadinium caudatum var. margalefii (first strain for the area) confirmed non‐toxigenicity of Atlantic populations of this species. One strain designated as Azadinium cf. zhuanum was similar to Az. zhuanum described from China but differed from the type strain in nucleus position, by the dominant number of apical plates, and by significant differences in rRNA gene sequences. Finally, two new non‐toxigenic Azadinium species are described from the North East Atlantic: Azadinium galwayense sp. nov. and Azadinium perfusorium sp. nov. Azadinium galwayense differed from other Azadinium by a characteristic combination regarding presence and location of the ventral pore (vp; on the right side of the pore plate), of a pyrenoid (located in the episome), and by a pentagonal shape of the median anterior intercalary plate 2a, and lack of contact between plates 1″ and 1a. Azadinium perfusorium shared the same vp position as Az. galwayense and differed by a characteristic combination of a pyrenoid located in the hyposome, a tetragonal shape of plate 2a, and a relatively large size of the two lateral anterior intercalary plates. Molecular phylogeny confirmed the distinctiveness of these two new species and their placement in Azadinium. The present findings significantly increased knowledge on the diversity of Azadinium species in the North East Atlantic.

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Wind‐driven currents and water masses shape spring phytoplankton distribution and composition in hydrologically complex, productive shelf waters

Ferronato

Berden

Rivarossa

et al. 2023

Limnology & Oceanography

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The Northern Patagonian shelf (35–42°S SW South Atlantic) is a transitional area between the subtropical and subantarctic domains, where the shelf shifts from source to sink of atmospheric CO2. In the southeast portion (39–42°S, 61–55°W) of this area, winds interact with different water masses and picture a complex circulation that supports two productive frontal systems: the Mid‐Shelf Front (MSF) and the Patagonian Shelf‐break Front. We combined remote sensing (e.g., monthly surface chlorophyll a over the period: September–December 2002–2021), reanalysis (winds and surface currents), and in situ measurements from three cross‐shelf cruises in spring 2016, 2017, and 2019, to assess the underlying drivers of phytoplankton distribution. The anomalous high chlorophyll concentration in the MSF in spring 2016 was related to enhanced water mass retention by N‐NE winds. Contrasting phytoplankton assemblages emerged related to stratified waters in the shelf influenced by the Patagonian Current and vertically mixed waters at the continental edge (Malvinas Current). Large diatoms prevailed in the outer shelf followed by dinoflagellates, while nanoflagellates and microzooplankton dominated in the mid‐shelf. Furthermore, thickness of the subsurface chlorophyll maximum was broader in the early spring than in a more advanced state of the bloom where the coccolithophore Emiliania huxleyi was abundant. We showed that different time‐scale physical processes—winds, surface currents and vertical mixing—are underpinning the development of spring blooms with different taxa composition in the Patagonian shelf, where different water masses define the hydrological complexity that led to patchy phytoplankton.

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High abundance of Amphidomataceae (Dinophyceae) during the 2015 spring bloom of the Argentinean Shelf and a new, non-toxigenic ribotype of Azadinium spinosum

Cited by 21 publications

References 63 publications

Distribution and abundance of azaspiracid-producing dinophyte species and their toxins in North Atlantic and North Sea waters in summer 2018

Distribution and abundance of azaspiracid-producing dinophyte species and their toxins in North Atlantic and North Sea waters in summer 2018

Morphological and molecular characterization of multiple new Azadinium strains revealed a high diversity of non‐toxigenic species of Amphidomataceae (Dinophyceae) including two new Azadinium species in Irish waters, North East Atlantic

Wind‐driven currents and water masses shape spring phytoplankton distribution and composition in hydrologically complex, productive shelf waters

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