Hierarchies of damage induced loss of mechanical properties in calcified bone after in vivo fatigue loading of rat ulnae

Macione, James; Kavukcuoglu, N.B.; Nesbitt, Robert Sterling; Mann, A.; Güzelsu, N.; Kotha, Shiva P.

doi:10.1016/j.jmbbm.2011.03.010

Cited by 11 publications

(7 citation statements)

References 44 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The contribution of microdamage accumulation to the reduction of fracture resistance has been evaluated in vitro by measuring changes in elastic modulus, strength, and fracture toughness (Macione et al. 2011). Microcracks have been shown to be associated with intracortical resorption in overuse and stress fractures, both in vivo and in vitro (O’Brien et al.…”

Section: Discussionmentioning

confidence: 99%

Women with previous stress fractures show reduced bone material strength

Sosa¹,

Eriksen²

2016

Acta Orthopaedica

View full text Add to dashboard Cite

Background and purpose — Bone fragility is determined by bone mass, bone architecture, and the material properties of bone. Microindentation has been introduced as a measurement method that reflects bone material properties. The pathogenesis of underlying stress fractures, in particular the role of impaired bone material properties, is still poorly understood. Based on the hypothesis that impaired bone material strength might play a role in the development of stress fractures, we used microindentation in patients with stress fractures and in controls.Patients and methods — We measured bone material strength index (BMSi) by microindentation in 30 women with previous stress fractures and in 30 normal controls. Bone mineral density by DXA and levels of the bone markers C-terminal cross-linking telopeptide of type-1 collagen (CTX) and N-terminal propeptide of type-1 procollagen (P1NP) were also determined.Results — Mean BMSi in stress fracture patients was significantly lower than in the controls (SD 72 (8.7) vs. 77 (7.2); p = 0.02). The fracture subjects also had a significantly lower mean bone mineral density (BMD) than the controls (0.9 (0.02) vs. 1.0 (0.06); p = 0.03). Bone turnover—as reflected in serum levels of the bone marker CTX—was similar in both groups, while P1NP levels were significantly higher in the women with stress fractures (55 μg/L vs. 42 μg/L; p = 0.03). There was no correlation between BMSi and BMD or bone turnover.Interpretation — BMSi was inferior in patients with previous stress fracture, but was unrelated to BMD and bone turnover. The lower values of BMSi in patients with previous stress fracture combined with a lower BMD may contribute to the increased propensity to develop stress fractures in these patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Women with previous stress fractures show reduced bone material strength

Sosa¹,

Eriksen²

2016

Acta Orthopaedica

View full text Add to dashboard Cite

show abstract

“…Then, the number of disrupted canaliculi (loss of canalicular fluorescence and discontinuously stained canaliculi) over the imaging stack of four images was counted, and the percentage of disrupted canaliculi was calculated. Several previous studies have also used the same method for obtaining the Z‐stack images of osteocyte canaliculi and quantifying the disrupted canaliculi over the imaging stack .…”

Section: Methodsmentioning

confidence: 99%

“…Several previous studies have also used the same method for obtaining the Z-stack images of osteocyte canaliculi and quantifying the disrupted canaliculi over the imaging stack. 8,[34][35][36][37] The intracortical resorption space in the accumulation region of linear microcracks was also imaged using a confocal microscope, and the resorption space density (Rs.Dn, ##/mm 2 ) was then quantified. 11,27 Moreover, normal osteocytes (fully occupy lacunae, N.Ot.)…”

Section: Spect/ct Scanningmentioning

confidence: 99%

Spatiotemporal Distribution of Linear Microcracks and Diffuse Microdamage Following Daily Bouts of Fatigue Loading of Rat Ulnae

Liu

Tang

Zhang

et al. 2019

Journal Orthopaedic Research

View full text Add to dashboard Cite

Microdamage accumulation contributes to impaired skeletal mechanical integrity. The bone can remove microdamage by initiating targeted bone remodeling. However, the spatiotemporal characteristics of microdamage initiation and propagation and their relationship with bone remodeling in response to fatigue loading, especially for more physiologically relevant daily bouts of compressive loading, remain poorly understood. The right forelimbs of 24 rats were cyclically loaded with a ramp waveform for 1,500 cycles/day, and contralateral ulnae were not loaded as the controls. The rats were divided into four equal groups and loaded for 1, 4, 7, and 10 days, respectively. We demonstrated that linear microcracking accumulation exhibited a non‐linear time‐varying process within 10 days of loading with peaked microcrack density at Day 7. Disrupted canaliculi surrounding linear microcracks showed high similarity with the temporal changes of linear microcracking accumulation. Observable intracortical resorption regions were found on Day 10. We found more linear microcracks accumulated in the tensile cortex, but longer cracks were observed in the compressive sides. Increased accumulation of diffuse microdamage was observed from Day 4, but no obvious peak was observed within the 10‐day loading period. Diffuse damage first initiated in the compressive cortices but extended to tension from Day 7. The diffuse damage exhibited no impacts on the surrounding osteocyte integrity. Together, our findings revealed a time‐dependent, bone remodeling‐mediated varying process of linear microcracking accumulation following daily bouts of fatigue loading (with observable peak at Day 7 under our loading regime). Our study also identified distinct spatial accumulation of linear and diffuse microdamage in rat ulnae with tensile and compressive strains. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2112–2121, 2019

show abstract

“…These techniques are still investigational for clinical use. Nanoindentation techniques measure similar properties of bone on a submicron scale and can be used on either tissue samples or histological slides. Nanoindentation has been used to evaluate the nanomechanical properties of the regenerating bone callus as well as the local tissue mechanical response around implants .…”

Section: Techniques Of Assessment: Preclinical Techniquesmentioning

confidence: 99%

Quality of bone healing: Perspectives and assessment techniques

Guda

Labella

Chan

et al. 2014

Wound Repair Regeneration

View full text Add to dashboard Cite

Bone regeneration and healing is an area of extensive research providing an ever-expanding set of not only therapeutic solutions for surgeons but also diagnostic tools. Multiple factors such as an ideal graft, the appropriate biochemical and mechanical wound environment, and viable cell populations are essential components in promoting healing. While bony tissue performs many functions, critical is mechanical strength, followed closely by structure. Many tools are available to evaluate bone quality in terms of quantity, structure, and strength; the purpose of this article is to identify the factors that can be evaluated and the advantages and disadvantages of each in assessing the quality of bone healing in both preclinical research and clinical settings.

show abstract

Hierarchies of damage induced loss of mechanical properties in calcified bone after in vivo fatigue loading of rat ulnae

Cited by 11 publications

References 44 publications

Women with previous stress fractures show reduced bone material strength

Women with previous stress fractures show reduced bone material strength

Spatiotemporal Distribution of Linear Microcracks and Diffuse Microdamage Following Daily Bouts of Fatigue Loading of Rat Ulnae

Quality of bone healing: Perspectives and assessment techniques

Contact Info

Product

Resources

About