Hierarchically structured seamless silk scaffolds for osteochondral interface tissue engineering

Singh, Yogendra; Moses, Joseph Christakiran; Bhunia, Bibhas K.; Nandi, Samit Kumar; Mandal, Biman B.

doi:10.1039/c8tb01344f

Cited by 38 publications

(30 citation statements)

References 63 publications

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“…The introduction of brushite cement variants did not drastically vary the pore size distribution, as all the scaffolds exhibited pore sizes ranging from 50−200 μm (Figure 1G) in comparison with control silk matrices (Figure 1B), which correlates with our previously published reports with pore sizes of similar range. 11,15,16 The presentation of open fibrous network is essential for cellular infiltration, migration of vascular cells, and immune cells that facilitate the initial graft recognition and further remodelling of the scaffold. 17 The presentation of biophysical cues such as stiffer or relatively rougher surface has been seen to maintain the osteogenic phenotype.…”

Section: Resultsmentioning

confidence: 99%

Synergistic Effects of Silicon/Zinc Doped Brushite and Silk Scaffolding in Augmenting the Osteogenic and Angiogenic Potential of Composite Biomimetic Bone Grafts

Moses

Dey

Devi

et al. 2019

ACS Biomater. Sci. Eng.

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Cell instructive scaffolding platforms displaying synergistic effects by virtue of their chemical and physical cues have tremendous scope in modulating cell phenotype and thus improving the success of any graft. In this regard, we report here the development of Si- and Zn-doped brushite cement composited with silk scaffolding that hierarchically emulated the cancellous bone. The composite scaffolds fabricated exhibited an open porous network capable of enhanced osteoblast survival as attested by increased alkaline phosphatase activity and also sustaining osteoclast activity affirmed by tartrate resistant acid phosphatase staining. Moreover, the chemical cues presented by dissolutions products from the composite scaffold enabled the osteoblasts to secrete proangiogenic factors which favored better endothelial cell survival, confirmed through in vitro experiments. Moreover, the efficacy of these composite biomimetic scaffolds was validated in vivo in volumetric femur defects in rabbits, which revealed that these matrices influenced vascular cell infiltration and favored the formation of matured bony plate. Fluorochrome labeling studies and microtomography analysis revealed that at the end of three months, the implanted composite scaffolds had completely resorbed, leaving behind neo-osseous tissue and vouching for clinical translation of these composite matrices as viable and affordable bone-graft substitutes.

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Section: Resultsmentioning

confidence: 99%

Synergistic Effects of Silicon/Zinc Doped Brushite and Silk Scaffolding in Augmenting the Osteogenic and Angiogenic Potential of Composite Biomimetic Bone Grafts

Moses

Dey

Devi

et al. 2019

ACS Biomater. Sci. Eng.

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“…[161,168,169] Molding can also be used to create a bilayer scaffold with a fiber-reinforced gel as the bottom layer by simply placing fibers in the bottom half of the mold before adding a polymer solution on top. [170] Molding also allows the fabrication of oriented structures, where polymer fibers or pores are arranged along a particular axis. For example, the application of a temperature gradient from the edges to the center of a mold, which was obtained by applying liquid nitrogen to a cylindrical mold with thermally insulated bases and core, allowed to create a radially oriented structure of collagen with the aim to facilitate cell infiltration from the edges of the defect.…”

Section: Implant Shapingmentioning

confidence: 99%

Material‐Assisted Strategies for Osteochondral Defect Repair

et al. 2022

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The osteochondral (OC) unit plays a pivotal role in joint lubrication and in the transmission of constraints to bones during movement. The OC unit does not spontaneously heal; therefore, OC defects are considered to be one of the major risk factors for developing long-term degenerative joint diseases such as osteoarthritis. Yet, there is currently no curative treatment for OC defects, and OC regeneration remains an unmet medical challenge. In this context, a plethora of tissue engineering strategies have been envisioned over the last two decades, such as combining cells, biological molecules, and/or biomaterials, yet with little evidence of successful clinical transfer to date. This striking observation must be put into perspective with the difficulty in comparing studies to identify overall key elements for success. This systematic review aims to provide a deeper insight into the field of material-assisted strategies for OC regeneration, with particular considerations for the therapeutic potential of the different approaches (with or without cells or biological molecules), and current OC regeneration evaluation methods. After a brief description of the biological complexity of the OC unit, the recent literature is thoroughly analyzed, and the major pitfalls, emerging key elements, and new paths to success are identified and discussed.

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“…It has been pointed out that using the adhesive the brittle substrate is toughened without effecting the porous structure [94] and the cellular infiltration between layers may be inhibited [96]; however, such bonding hardly provides cohesion strength comparable to that by suture bonding [81]. It is urgent to address the severe problems caused by traditional preparation strategies: the chondrocyte-induced OC scaffolds lack layered structures to mimic heterogeneous OC tissue and cannot prevent osteoblasts from migrating into cartilage layer; the assembly synthesis method without an adequate ability to integrate biphasic scaffolds easily leads to in vivo collapse/stratification of the implants during natural degradation [97,98].…”

Section: Fabricating Strategies Of Biomimetic Oc Scaffoldsmentioning

confidence: 99%

Current researches on design and manufacture of biopolymer-based osteochondral biomimetic scaffolds

et al. 2021

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Currently, osteochondral (OC) tissue engineering has become a potential treatment strategy in repairing chondral lesions and early osteoarthritis due to the limited self-healing ability of cartilage. However, it is still challenging to ensure the integrity, physiological function and regeneration ability of stratified OC scaffolds in clinical application. Biomimetic OC scaffolds are attractive to overcome the above problems because of their similar biological and mechanical properties with native OC tissue. As a consequence, the researches on biomimetic design to achieve the tissue function of each layer, and additive manufacture (AM) to accomplish composition switch and ultrastructure of personalized OC scaffolds have made a remarkable progress. In this review, the design methods of biomaterial and structure as well as computer-aided design, and performance prediction of biopolymer-based OC scaffolds are presented; then, the characteristics and limitations of AM technologies and the integrated manufacture schemes in OC tissue engineering are summarized; finally, the novel biomaterials and techniques and the inevitable trends of multifunctional bio-manufacturing system are discussed for further optimizing production of tissue engineering OC scaffolds.

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Hierarchically structured seamless silk scaffolds for osteochondral interface tissue engineering

Abstract: Development of a hierarchically analogous biphasic scaffold fabricated in a facile and minimalistic method for repair of osteochondral defect.

Cited by 38 publications

References 63 publications

Synergistic Effects of Silicon/Zinc Doped Brushite and Silk Scaffolding in Augmenting the Osteogenic and Angiogenic Potential of Composite Biomimetic Bone Grafts

Synergistic Effects of Silicon/Zinc Doped Brushite and Silk Scaffolding in Augmenting the Osteogenic and Angiogenic Potential of Composite Biomimetic Bone Grafts

Material‐Assisted Strategies for Osteochondral Defect Repair

Current researches on design and manufacture of biopolymer-based osteochondral biomimetic scaffolds

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