Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell

Skopalová, Kateřina; Radaszkiewicz, Katarzyna Anna; Kadlečková, Markéta; Pachernı́k, Jiřı́; Minařík, Antonín; Capáková, Zdenka; Kašpárková, Věra; Mráček, Aleš; Daďová, Eliška; Humpolíček, Petr

doi:10.3390/ijms222111943

Cited by 3 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on previous research ,− as well as additional studies, , we hypothesized that the characteristics of the resulting surface texture could be influenced by various factors such as temperature, rotational speed, and IPA/ n BT ratio. These factors will be further discussed.…”

Section: Resultsmentioning

confidence: 99%

“…Phase Inversion Modification of the HU Surface. Similar to the case of PS, 40 the addition of HU to the modification solution allowed for preparation of new surface texture types. From a physicochemical point of view, this approach corresponds to the phase inversion described in the literature.…”

Section: Texturing Of the Hu Surface By Phase Separationmentioning

confidence: 99%

“…43−45 Due to the structural and conformational differences between PS and HU, the modification process of the polysaccharide was much more complex and did not allow for the preparation of bulk porous structures as in the case of synthetic polymers. 40 A key point in the modification was to find out a suitable ratio of the components of the mixture, correct surface rotation speed, amount and volume of the dosed mixture, dose frequency, and especially content of residual water in the HU pre-swollen layer.…”

Section: Texturing Of the Hu Surface By Phase Separationmentioning

confidence: 99%

See 2 more Smart Citations

Controlled Structuring of Hyaluronan Films by Phase Separation and Inversion

Smolka,

Kadlečková,

Kocourková

et al. 2023

Langmuir

Self Cite

View full text Add to dashboard Cite

This work explores application of phase separation phenomena for structuring of films made from hyaluronan. A timesequenced dispensing of different solution mixtures was applied under rotation of hyaluronan-covered substrates to generate surface textures. This method is applicable in direct surface modification or cover layer deposition. Changes in the surface topography were characterized by atomic force microscopy, optical microscopy, and contact and non-contact profilometry. The mechanical properties of the surface-modified self-supporting films were compared using a universal testing machine. Experimental results show that diverse hyaluronan-based surface reliefs and self-supporting films with improved mechanical properties can be prepared using a newly designed multi-step phase separation process without the need for sacrificial removable templates or additives.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Texturing Of the Hu Surface By Phase Separationmentioning

confidence: 99%

Section: Texturing Of the Hu Surface By Phase Separationmentioning

confidence: 99%

See 1 more Smart Citation

Controlled Structuring of Hyaluronan Films by Phase Separation and Inversion

Smolka,

Kadlečková,

Kocourková

et al. 2023

Langmuir

Self Cite

View full text Add to dashboard Cite

show abstract

“…We found a strong correlation between these two, in the sense that the morphology of the cells is severely influenced by the environmental conditions, which act as a key factor in cellular differentiation [ 54 , 56 ]. The architecture of the cell culture substrate has been previously shown to influence cells differentiation [ 57 , 58 , 59 ]. Here, the elongated and flattened polygonal shape of the OLN 93 cells grown on the glass substrate ( Figure 1 a), is associated with the differentiation of cells towards the mature form in which they fulfill their physiological function of producing the myelin sheath [ 56 ].…”

Section: Resultsmentioning

confidence: 99%

Laser Direct Writing of Dual-Scale 3D Structures for Cell Repelling at High Cellular Density

Păun

Călin

Popescu

et al. 2022

IJMS

View full text Add to dashboard Cite

The fabrication of complex, reproducible, and accurate micro-and nanostructured interfaces that impede the interaction between material’s surface and different cell types represents an important objective in the development of medical devices. This can be achieved by topographical means such as dual-scale structures, mainly represented by microstructures with surface nanopatterning. Fabrication via laser irradiation of materials seems promising. However, laser-assisted fabrication of dual-scale structures, i.e., ripples relies on stochastic processes deriving from laser–matter interaction, limiting the control over the structures’ topography. In this paper, we report on laser fabrication of cell-repellent dual-scale 3D structures with fully reproducible and high spatial accuracy topographies. Structures were designed as micrometric “mushrooms” decorated with fingerprint-like nanometric features with heights and periodicities close to those of the calamistrum, i.e., 200–300 nm. They were fabricated by Laser Direct Writing via Two-Photon Polymerization of IP-Dip photoresist. Design and laser writing parameters were optimized for conferring cell-repellent properties to the structures, even for high cellular densities in the culture medium. The structures were most efficient in repelling the cells when the fingerprint-like features had periodicities and heights of @200 nm, fairly close to the repellent surfaces of the calamistrum. Laser power was the most important parameter for the optimization protocol.

show abstract