Hierarchical porous and hydrophilic metal–organic frameworks with enhanced enzyme activity

Abstract

Metal–organic frameworks (MOFs) for enzyme encapsulation induced biomimetic mineralization are commonly microporous and hydrophobic, which result in a rather high mass transfer resistance of the reactants and restrain the enzyme catalytic activity.

Convenient Immobilization of α‐L‐Rhamnosidase on Cerium‐based Metal‐Organic Frameworks Nanoparticles for Enhanced Enzymatic Activity and Recyclability

“…The k cat value for Rha@Ce‐BTC was calculated to be 4.3×10 5 s −1 , higher than the value of free Rha of 3.9×10 5 s −1 . The enhanced catalytic activity can be ascribed to two factors, (i) the appropriate conformation of enzymes facing toward the substrates promoted by Ce‐BTC support, [3,53] (ii) Ce‐BTC facilitates the enrichment of reaction substrate or enhances substrate transportation via the increased affinity of the substrate [31] . However, we also found that Ce 3+ did not manifest the activation effect of the enzyme.…”

“…The water contact angle analysis showed that ZIF‐8, t‐Ce‐BTC, m‐Ce‐BTC, MIL‐101(Cr), MIL‐88B(Fe), and UiO‐66(Zr) were 80.0°, 8.7°, 15.4°, 5.3°, 13.6°, and 11.6°, respectively (Figure S15), which confirms that ZIF‐8 displays relative hydrophobicity nature as compared to the other five MOFs. The hydrophobic ZIF‐8 might induce the aggregation of Rha and change the conformation, resulting in the loss of enzyme activity [3,44] . In addition, Rha@MIL‐88B(Fe), Rha@MIL‐101(Cr), and Rha@UiO‐66 (Zr) did not exhibit fluorescence peaks at around 353 nm (Figure S14b‐d), which might be due to that the parent MOFs themselves can emit fluorescence [47–49] .…”

“…Enzymes are proteins produced in living cells, which are critical to the process of metabolism with high efficiency, specificity, and (regio‐, stereo‐, and chemo‐) selectivity in biocatalysis reactions [1–3] . α‐L‐Rhamnosidase (EC 3.2.1.40, denoted as Rha) is a well‐known glycoside hydrolase, that can specifically hydrolyze a variety of natural products ( e. g ., naringin, rutin, terpene glycosides, and hesperidin) with low activation energy [4–7] .…”

Microfluidic fabrication of hydrogel microparticles with MOF-armoured multi-enzymes for cascade biocatalytic reactions

“…This result is consistent with previous reports, in which their enzyme-loaded ZIF-8 nanoparticles exhibit largely reduced enzymatic activity at high temperatures such as 60 °C and 80 °C. 42,43 By contrast, the microparticles maintain good enzymatic activity after treatment with boiling water (Fig. 8a), with a relative activity of >85% (Fig.…”

Metal−Organic Frameworks for Liquid Phase Applications

“…Microperoxidase-11 Tb-mesoMOF [ 478] Hem protein cytochrome c Tb-mesoMOF [ 479] GFPs, RFPs Hairy Cu-MOF [ 480] HeLa (human cervical cancer) cells DNA-UiO66 conjugate [ 481] Cytochrome c MN 3 (NDC) 3 DMF 4 [ 482] Cytochrome c ZIF-8 [ 483] 3D DNA M 6 L 4 sphere framework [ 484] Ferritin, glucose oxidase HKUST-1 film [ 485] HRP, cytochrome c, MP-11 PCN-333(Al) [ 486] Myoglobin, green fluorescent protein IRMOF-74 [ 487] Catalase ZIF-90 Glucose oxidase, horseradish peroxidase ZIF-8 [ 491] Urease ZIF-8 [ 492] Phospholipid bilayers Zr-NMOFs [ 493] Catalase ZIF-90 and ZIF-8 [ 494] -chymotrypsin Cu(bpy)2(OTf) 2 [ 495] Circulating tumor cells ZnMOF-COOH [ 496] Diphenylalanine Cu 2 L 2 ted [ 497] Diphenylalanine N 3 -bio-MOF-100 [ 498] HIV DNA, thrombin Cu(H 2 dtoa) [ 499] Cytochrome c peroxidase CuBDC [ 496] Lipase PS COF-OMe, PCN-128y [ 500] Bovine serum albumin PVP coated ZIF-8 NPs [ 501] Protein biomarkers in urine, serum, plasma, and blood ZIF-8 [ 502] Fatty acids MIL-101 and ZIF-8 [ 503] Enzyme ZIF-8 [ 504] Biomolecule separation Glycopeptides cross-linked CD-MOFs (LCD-MOFs) [ 511] Histidine-rich proteins, hemoglobin from a protein mixture and human blood samples Glycopeptides MG@Zn-MOFs [ 517] 33 glycopeptides MIL-101(Cr)-maltose [ 518] Angiotensin II (peptide) and BSA (protein) MGMOF [ 519] Human serum digest peptides Fe 3 O 4 /C@MIL-100(Fe) [ 520] BSA tryptic digest Fe 3 O 4 @PDA@ZIF-8 [ 521] Glycopeptides MIL-101(NH 2 )@Au-Cys Phosphopeptides of -casein DZMOF [526] Phosphopeptides from tilapia eggs Fe 3 O 4 /MIL-101(Fe) [527] Phosphopeptides in a tryptic digest of -casein MIL-101(Cr)-UR2 …”