Hierarchical Binding of the TodT Response Regulator to Its Multiple Recognition Sites at the tod Pathway Operon Promoter

Lacal, Jesús; Guazzaroni, María‐Eugenia; Büsch, Andreas; Krell, Tino; Ramos, Juan L.

doi:10.1016/j.jmb.2007.12.004

Cited by 29 publications

(29 citation statements)

References 66 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these is the TodS/TodT TCS, which controls the expression of the tod catabolic genes, enabling this strain to use toxic compounds such as benzene, toluene, and ethylbenzene as sole carbon sources (10,13,15,17,21). The sensor kinase TodS has a complex domain arrangement containing two functional modules, each harboring a sensor domain (PAS-1 and PAS-2) and an autokinase domain (HK1 and HK2) separated by a receiver domain (RRR) (2,3,11). HK1, RRR, and HK2 each contain a phosphorylatable residue, namely, H190, D500, and H760, respectively (2).…”

mentioning

confidence: 99%

“…HK1, RRR, and HK2 each contain a phosphorylatable residue, namely, H190, D500, and H760, respectively (2). The TodT protein, on the other hand, shows the classic arrangement of an N-terminal input or phosphorylation site (D57) and a C-terminal helix-turn-helix (HTH) motif containing a DNA-binding domain (9,11,12).…”

mentioning

confidence: 99%

“…A functional model indicated that IHF favors contact between the TodT activator, bound farther upstream, and the ␣ subunit of the RNA polymerase bound to the downstream promoter element. Once the scaffold is created and TodT is phosphorylated, the tod operon is transcribed efficiently (10,11,12,13).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Catabolite Repression of the TodS/TodT Two-Component System and Effector-Dependent Transphosphorylation of TodT as the Basis for Toluene Dioxygenase Catabolic Pathway Control

et al. 2010

Self Cite

View full text Add to dashboard Cite

The TodS/TodT two-component system of Pseudomonas putida regulates the expression of the toluene dioxygenase (tod) operon for the metabolism of toluene, benzene, and ethylbenzene. The sensor kinase TodS has a complex domain arrangement containing two functional modules, each harboring a sensor and an autokinase domain separated by a receiver domain. The TodT protein is the cognate response regulator that activates transcription of the toluene dioxygenase (TOD) pathway genes at the P todX promoter. We report in this study that the todST operon is transcribed from a main promoter and that the ؉1 initiation point is located 31 nucleotides upstream from the A of the first ATG codon and is preceded by a ؊10/؊35 canonical promoter. Expression from P todS is under catabolite control, and in cells growing with glucose, the level of expression from this promoter is reduced, which in turn translates to low levels of the TodS/TodT regulators and results in a decrease of transcription from the P todX promoter. Thus, the main underlying regulatory mechanisms of the tod structural genes are at the levels of catabolite repression control from P todS and transcription activation, mediated by the TodT response regulator through a regulatory cascade in which the effector enhances autophosphorylation of TodS by ATP, with subsequent transphosphorylation of TodT.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Catabolite Repression of the TodS/TodT Two-Component System and Effector-Dependent Transphosphorylation of TodT as the Basis for Toluene Dioxygenase Catabolic Pathway Control

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…Hierarchical promoter occupancy can concern either the same TF (Lacal et al, 2008) or different types of TFs (Archer et al, 1992;Chávez and Beato, 1997). Hierarchical equilibration has been proposed in the very different context of hemoglobin oxygenation (Michel, 2008) but it is likely to be relatively rare for carriers and enzymes.…”

Section: Hierarchical Binding Cooperativitymentioning

confidence: 99%

How transcription factors can adjust the gene expression floodgates

Michel

2010

Progress in Biophysics and Molecular Biology

View full text Add to dashboard Cite

show abstract

“…17) McpT of P. putida DOT-T1E mediates the chemotactic response to toluene and other aromatic hydrocarbons. 18) NtdY of the Acidovorax sp. JS42 m is involved in its response to 2-nitrotoluene.…”

mentioning

confidence: 99%

A putative porin gene of Burkholderia sp. NK8 involved in chemotaxis toward β-ketoadipate

Yamamoto-Tamura

Kawagishi

Ogawa

et al. 2015

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Burkholderia sp. NK8 can utilize 3-chlorobenzoate (3CB) as a sole source of carbon because it has a megaplasmid (pNK8) that carries the gene cluster (tfdT-CDEF) encoding chlorocatechol-degrading enzymes. The expression of tfdT-CDEF is induced by 3CB. In this study, we found that NK8 cells were attracted to 3CB and its degradation products, 3- and 4-chlorocatechol, and β-ketoadipate. Capillary assays revealed that a pNK8-eliminated strain (NK82) was defective in chemotaxis toward β-ketoadipate. The introduction of a plasmid carrying a putative outer membrane porin gene, which we name ompNK8, into strain NK82 restored chemotaxis toward β-ketoadipate. RT-PCR analyses demonstrated that the transcription of the ompNK8 gene was enhanced in the presence of 3CB.

show abstract

Hierarchical Binding of the TodT Response Regulator to Its Multiple Recognition Sites at the tod Pathway Operon Promoter

Cited by 29 publications

References 66 publications

Catabolite Repression of the TodS/TodT Two-Component System and Effector-Dependent Transphosphorylation of TodT as the Basis for Toluene Dioxygenase Catabolic Pathway Control

Catabolite Repression of the TodS/TodT Two-Component System and Effector-Dependent Transphosphorylation of TodT as the Basis for Toluene Dioxygenase Catabolic Pathway Control

How transcription factors can adjust the gene expression floodgates

A putative porin gene of Burkholderia sp. NK8 involved in chemotaxis toward β-ketoadipate

Contact Info

Product

Resources

About