Hidden in our pockets: building of a DNA barcode library unveils the first record of Myotis alcathoe for Portugal

Rebelo, Hugo; Ferreira, Sónia; Amorim, Francisco; Horta, Pedro; Raposeira, Helena; Santos, Helena; Beja, Pedro; Mata, Vanessa A.

doi:10.3897/bdj.8.e54479

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…This was done to validate the identity of cryptic species and of guano pellets collected from roosts, allowing the clear identification of all bat species occurring in Portugal (Rebelo et al. 2020), with the exception of Myotis myotis and Myotis blythii , which were therefore treated as a species complex due to well known issues of mitochondrial introgression (Afonso et al. 2017).…”

Section: Methodsmentioning

confidence: 99%

“…Bat field identifications were confirmed by amplifying a small COI fragment using the bat specific primers SFF_145f-351r (Walker et al 2016), also adapted to be sequenced with an Illumina machine. This was done to validate the identity of cryptic species and of guano pellets collected from roosts, allowing the clear identification of all bat species occurring in Portugal (Rebelo et al 2020), with the exception of Myotis myotis and Myotis blythii, which were therefore treated as a species complex due to well known issues of mitochondrial introgression (Afonso et al 2017). PCR reactions and cycling conditions were similar to that of prey, except that MyTaq Mix (Bioline, Porto, Portugal) was used, and annealing was performed at 56°C.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

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Combining DNA metabarcoding and ecological networks to inform conservation biocontrol by small vertebrate predators

Mata

Silva

Veríssimo

et al. 2021

Ecological Applications

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In multifunctional landscapes, diverse communities of flying vertebrate predators provide vital services of insect pest control. In such landscapes, conservation biocontrol should benefit service‐providing species to enhance the flow, stability and resilience of pest control services supporting the production of food and fiber. However, this would require identifying key service providers, which may be challenging when multiple predators interact with multiple pests. Here we provide a framework to identify the functional role of individual species to pest control in multifunctional landscapes. First, we used DNA metabarcoding to provide detailed data on pest species predation by diverse predator communities. Then, these data were fed into an extensive network analysis, in which information relevant for conservation biocontrol is gained from parameters describing network structure (e.g., modularity) and species roles in such network (e.g., centrality, specialization). We applied our framework to a Mediterranean landscape, where 19 bat species were found to feed on 132 insect pest species. Metabarcoding data revealed potentially important bats that consumed insect pest species in high frequency and/or diversity. Network analysis showed a modular structure, indicating sets of bat species that are required to regulate specific sets of insect pests. A few generalist bats had particularly important roles, either at network or module levels. Extinction simulations highlighted six bats, including species of conservation concern, which were sufficient to ensure that over three‐quarters of the pest species had at least one bat predator. Combining DNA metabarcoding and ecological network analysis provides a valuable framework to identify individual species within diverse predator communities that might have a disproportionate contribution to pest control services in multifunctional landscapes. These species can be regarded as candidate targets for conservation biocontrol, although additional information is needed to evaluate their actual effectiveness in pest regulation.

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Section: Methodsmentioning

confidence: 99%

Section: Laboratory Proceduresmentioning

confidence: 99%

Combining DNA metabarcoding and ecological networks to inform conservation biocontrol by small vertebrate predators

Mata

Silva

Veríssimo

et al. 2021

Ecological Applications

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“…Also on the European level, distribution of this species is full of knowledge gaps, mainly due to under-sampling of the habitats of this bat species and misidentifications during fieldwork (Jan et al 2010;Bashta et al 2011). For example, the presence of M. alcathoe in Portugal was also confirmed using DNA barcoding, after an individual, morphologically determined as M. mystacinus, was analysed molecularly (Rebelo et al 2020).…”

Section: Vespertilionidae Myotis Sppmentioning

confidence: 99%

Study of bats in the upper Neretva River valley (Bosnia and Herzegovina)

Zagmajster,

Milanović,

Rexhepi

et al. 2023

Nat Slov

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The upper Neretva River valley with river and tributaries, preserved forests, natural caves and buildings represents a very interesting area for bats. During two field expeditions in summer 2022, we collected data on bats using a variety of methods: inspecting the potential roosts, mist netting, and recording bat echolocation calls. We used manual and automatic ultrasound detectors most frequently at sites along and near the Neretva River. The morphological identification of some species was confirmed using DNA analyses. We detected at least 13 bat species in the study area extending from Krupac to Konjic. With one addition, known from the literature, 14 different bat species have been found in the upper Neretva River valley, presenting nearly a half of all bat species recorded in Bosnia and Herzegovina. We confirmed the presence of three species of the highest conservation concern in Europe, listed in the Annex II of the Habitats directive: lesser horseshoe bat (Rhinolophus hipposideros), greater horseshoe bat (Rhinolophus ferrumequinum), and greater mouse-eared bat (Myotis myotis). Our finding of the alcathoe bat (Myotis alcathoe) presents new confirmation of this species in Bosnia and Herzegovina. Also, observation of the parti-coloured bat (Vespertilio murinus) is one of the few records of the species in this country. The upper Neretva River valley can be considered as an area of high conservation importance for bats. Additional fieldwork, including during other seasons, would likely reveal more bat species and their sites in the area of the upper Neretva River valley.

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“…The InBIO Barcoding Initiative (IBI) makes use of High-Throughput Sequencing technologies to construct a reference collection of morphologically identified Portuguese specimens and respective DNA barcodes (e.g. Rebelo et al 2020 , Ferreira et al 2021 , Oliveira et al 2021 , Pauperio et al 2023 , Rosa et al 2023 ). DNA barcoding offers a rapid, cost-effective alternative tool for both the identification of described species and the discovery of new ones ( Hebert et al 2003 , Savolainen et al 2005 , Mitchell 2008 , our papers).…”

Section: Introductionmentioning

confidence: 99%

The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese moths

Ferreira,

Corley,

Nunes

et al. 2024

BDJ

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The InBIO Barcoding Initiative (IBI) Dataset - DS-IBILP08 contains records of 2350 specimens of moths (Lepidoptera species that do not belong to the superfamily Papilionoidea). All specimens have been morphologically identified to species or subspecies level and represent 1158 species in total. The species of this dataset correspond to about 42% of mainland Portuguese Lepidoptera species. All specimens were collected in mainland Portugal between 2001 and 2022. All DNA extracts and over 96% of the specimens are deposited in the IBI collection at CIBIO, Research Center in Biodiversity and Genetic Resources. The authors enabled "The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese moths" in order to release the majority of data of DNA barcodes of Portuguese moths within the InBIO Barcoding Initiative. This dataset increases the knowledge on the DNA barcodes of 1158 species from Portugal belonging to 51 families. There is an increase in DNA barcodes of 205% in Portuguese specimens publicly available. The dataset includes 61 new Barcode Index Numbers. All specimens have their DNA barcodes publicly accessible through BOLD online database and the distribution data can be accessed through the Global Biodiversity Information Facility (GBIF).

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Hidden in our pockets: building of a DNA barcode library unveils the first record of Myotis alcathoe for Portugal

Cited by 7 publications

References 31 publications

Combining DNA metabarcoding and ecological networks to inform conservation biocontrol by small vertebrate predators

Combining DNA metabarcoding and ecological networks to inform conservation biocontrol by small vertebrate predators

Study of bats in the upper Neretva River valley (Bosnia and Herzegovina)

The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese moths

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