Hidden Diversity of Crown Rust Resistance within Genebank Resources of Avena sterilis L.

Paczos-Grzęda, Edyta; Boczkowska, Maja; Sowa, Sylwia; Koroluk, Aneta; Toporowska, Joanna

doi:10.3390/agronomy11020315

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…Modern oat breeding prioritises high yield and other desirable agronomic traits, including resistance to biotic and abiotic stress. Wild oat progenitors have been proven to be a rich source of useful genes [35][36][37][38]; however, all Avena spp. are grouped into three gene pools [39], and successful transfer of genes from diploids or tetraploids from the secondary and tertiary gene pool to hexaploid A. sativa is more demanding and requires special techniques [40].…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Progenies Derived from Bidirectional Avena sativa L. and Avena fatua L. Crosses

2022

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Crossing genetically distant forms is widely used in breeding programs and allows the introduction of beneficial features from the wild into cultivated species. In this study, agronomic traits of two F2 segregating populations derived from crosses in both directions between A. sativa cv. Sam and A. fatua ‘51532’, as well as their parental forms, were phenotyped and statistically compared. Almost all mean values of the analysed features in the populations ranged between the values of the two parental forms. In both F2 populations, high variability of the traits was observed. The plant height was strongly correlated with the length of the first inernode below the panicle in all populations except parental form ‘51532’. An intermediate correlation between the plant height and the panicle length could be observed only for ‘Sam’ and ‘Sam’ × ‘51532’. The segregation of non-shattering to shattering phenotypes of the progeny confirmed a single gene inheritance of the trait. Additionally, in both combinations, transgressive forms in terms of some phenotypic traits were observed. The direction of crosses had no impact on the values of characterised parameters. During the interspecific crosses, new breeding lines and cultivars are obtained, enriched with specific qualitative and quantitative properties. The genetic distinctness between crossed species often prevents the formation of desired hybrids but also enables obtaining superior genotypes with traits exceeding the parental forms. Even though efficient crossbreeding with A. fatua is demanding, looking for new germplasm in wild ancestors is crucial for expanding the Avena genetic pool and developing long-term strategies beneficial to modern oat breeding.

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Section: Discussionmentioning

confidence: 99%

Characteristics of Progenies Derived from Bidirectional Avena sativa L. and Avena fatua L. Crosses

2022

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“…The level of heterogeneity of individual accessions estimated in this study should facilitate further research and indicate that the analysis of a single plant or SSD line may have limited effectiveness due to the large number of genotypes present in a single accession. The general evaluation of accessions, as commonly used by genebanks, may also be of limited effectiveness, as a heterogeneous accession with poor overall tolerance to biotic or abiotic stresses may contain individuals with an exceptional combination of genetic variants, but their unique value is masked by the presence of other genotypes [126]. The results obtained here should be compared with earlier studies [55,64] in order to determine to what extent Moroccan landraces constitute a separate gene pool from Polish cultivars and landraces.…”

Section: Water Deficit Adaptation and Its Potential For Breedingmentioning

confidence: 92%

Characterization of the Moroccan Barley Germplasm Preserved in the Polish Genebank as a First Step towards Selecting Forms with Increased Drought Tolerance

Boczkowska,

Puchta-Jasińska,

Bolc

et al. 2023

IJMS

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In marginal, arid, and semi-arid areas of Morocco, crops are often exposed to multiple abiotic and biotic stresses that have a major impact on yield. Farmer-maintained Moroccan landraces have been shaped by the impact of very strong selection pressures, gradually adapting to the local ecosystem and obsolete low-input agricultural practices without improvement towards high yield and quality. Considering the increasing threat of drought in Poland, it is necessary to introduce germplasm with tolerance to water deficit into barley breeding programs. The aim of this research was a DArTseq-based genetic characterization of a collection of germplasm of Moroccan origin, conserved in the Polish genebank. The results showed that all conserved landraces have a high level of heterogeneity and their gene pool is different from the material developed by Polish breeders. Based on the analysis of eco-geographical data, locations with extremely different intensities of drought stress were selected. A total of 129 SNPs unique to accessions from these locations were identified. In the neighborhood of the clusters of unique SNPs on chromosomes 5H and 6H, genes that may be associated with plant response to drought stress were identified. The results obtained may provide a roadmap for further research to support Polish barley breeding for increased drought tolerance.

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“…The obtained results will provide valuable data necessary for a comprehensive analysis of gene expression in oat. The study will also constitute a reference point for further analyses of the new Pc genes identified in our previous research 28 – 31 .…”

Section: Introductionmentioning

confidence: 91%

Validation of reference genes as an internal control for studying Avena sativa–Puccinia coronata interaction by RT-qPCR

Sowa

Sozoniuk

Toporowska

et al. 2022

Sci Rep

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In this study we evaluated eleven candidate reference genes in Avena sativa during compatible and incompatible interactions with two different pathotypes of Puccinia coronata f. sp. avenae in six time points post-inoculation. The identification of genes with high expression stability was performed by four algorithms (geNorm, NormFinder, BestKeeper and ΔCt method). The results obtained confirmed that the combination of two genes would be sufficient for reliable normalization of the expression data. In general, the most stable in the tested plant-pathogen system were HNR (heterogeneous nuclear ribonucleoprotein 27C) and EF1A (elongation factor 1-alpha). ARF (ADP-ribosylation factor) and EIF4A (eukaryotic initiation factor 4A-3) could also be considered as exhibiting high expression stability. CYP (cyclophilin) was shown by all assessment methods to be the worst candidate for normalization in this dataset. To date, this is the first report of reference genes selection in A. sativa–P. coronata interaction system. Identified reference genes enable reliable and comprehensive RT-qPCR analysis of oat gene expression in response to crown rust infection. Understanding the molecular mechanisms involved in the host–pathogen interactions may expand knowledge of durable resistance strategies beneficial to modern oat breeding.

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Hidden Diversity of Crown Rust Resistance within Genebank Resources of Avena sterilis L.

Cited by 6 publications

References 29 publications

Characteristics of Progenies Derived from Bidirectional Avena sativa L. and Avena fatua L. Crosses

Characteristics of Progenies Derived from Bidirectional Avena sativa L. and Avena fatua L. Crosses

Characterization of the Moroccan Barley Germplasm Preserved in the Polish Genebank as a First Step towards Selecting Forms with Increased Drought Tolerance

Validation of reference genes as an internal control for studying Avena sativa–Puccinia coronata interaction by RT-qPCR

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