Hic74, a novel alanine and glycine rich matrix protein related to nacreous layer formation in the mollusc Hyriopsis cumingii

Liu, Xiao Jun; Jin, Can; Wu, Leiming; Dong, Shaojian; Zeng, Shimei; Li, Jiale

doi:10.1016/j.aaf.2017.03.005

Cited by 13 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This protein was also securely identified in all of the freshwater unionoid reference shells (coverage varying from 34% in M. margaritifera , supported by 67 peptides, to 50% in U. crassus, supported by 203 peptides). Hic74 is an acidic, Ala- and Gly-rich shell matrix protein (Liu et al, 2017a). Consisting of 19 poly-A blocks, GA repeats, short acidic motifs (that probably bind to the mineral) and a GS-rich domain at the C-terminus (which resembles that of lustrin-A), this silk fibroin-like protein is likely to play a structural role in nacre formation and in enhancing its mechanical properties (Liu et al, 2017a).…”

Section: Resultsmentioning

confidence: 99%

“…Hic74 (GenBank: ARG42316.1) is an acidic (IP 4.68), alanine and glycine rich matrix protein, supposedly involved in nacreous layer formation (Liu et al, 2017a). Its structure consists of poly-alanine blocks (at least 17) with short acidic motifs, a ‘GS loop’ type coil structure of lustrin A (Wustman et al, 2002) at the C-terminus, the C-terminal 30 residues of which consist of short acidic-basic motifs.…”

Section: 1 Archaeological Sites and Double-buttonsmentioning

confidence: 99%

See 1 more Smart Citation

'Palaeoshellomics’ reveals the use of freshwater mother-of-pearl in prehistory

Sakalauskaite

Andersen

Biagi

et al. 2019

eLife

View full text Add to dashboard Cite

The extensive use of mollusc shell as a versatile raw material is testament to its importance in prehistoric times. The consistent choice of certain species for different purposes, including the making of ornaments, is a direct representation of how humans viewed and exploited their environment. The necessary taxonomic information, however, is often impossible to obtain from objects that are small, heavily worked or degraded. Here we propose a novel biogeochemical approach to track the biological origin of prehistoric mollusc shell. We conducted an in-depth study of archaeological ornaments using microstructural, geochemical and biomolecular analyses, including ‘palaeoshellomics’, the first application of palaeoproteomics to mollusc shells (and indeed to any invertebrate calcified tissue). We reveal the consistent use of locally-sourced freshwater mother-of-pearl for the standardized manufacture of ‘double-buttons’. This craft is found throughout Europe between 4200–3800 BCE, highlighting the ornament-makers’ profound knowledge of the biogeosphere and the existence of cross-cultural traditions.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: 1 Archaeological Sites and Double-buttonsmentioning

confidence: 99%

'Palaeoshellomics’ reveals the use of freshwater mother-of-pearl in prehistory

Sakalauskaite

Andersen

Biagi

et al. 2019

eLife

View full text Add to dashboard Cite

show abstract

“…Furthermore, we identified one marker peak (m/z 1570.8), that likely corresponds to a peptide shared by the two Unionida shells (freshwater bivalves) Unio pictorum (family Unionidae) and Pseudunio auricularius (family Margaritiferidae). We suggest that the peptide at m/ z 1570.8 (Table 2) belongs to protein Hic74 [64], which was found to be the dominant protein in unionoid shells [16]. The peak can be assigned to peptide sequence EAD(-18.01)DLALLSLLFGGR and it was previously identified by LC-MS/MS analyses.…”

Section: The Application Of "Palaeoshellomics": Shell Pmfsmentioning

confidence: 76%

Shell palaeoproteomics: First application of peptide mass fingerprinting for the rapid identification of mollusc shells in archaeology

Sakalauskaite

Marin

Pergolizzi

et al. 2020

Journal of Proteomics

View full text Add to dashboard Cite

HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

show abstract

“…Meanwhile, Webster et al (1999) reported that compounding animal and plant protein sources with additional amino acid profiles may enhance the possibility of nutrient inadequacy that could adversely affect fish growth. Fishmeal is successfully substituted with other vegetable protein sources in fish feeds, there is some concern that the current evaluations of essential amino acid requirements are unsatisfactory goal (Furuya et al, 2004), Some AAs may also turn into a limiting factor and thus need to be supplemented in feed for important species of fish (El-Sayed, 2014;Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The effect of replacement of fish meal with Amino Acids and Optimized Protein Levels in the diet of the Nile Tilapia Oreochromis niloticus

et al. 2019

View full text Add to dashboard Cite

This study was conducted to determine an appropriate replacement of fishmeal with amino acids (AAs) and optimized protein levels in practical diets for Oreochromis niloticus with mean initial body weight 12.52±0.63g. Six experimental and a control diet (total 7 diets) divided into two groups, and a control diet (D1) containing 32% protein. The first group contained three diets that included different dietary protein levels, viz. 20 (D2), 25 (D3), and 30% (D4) with AAs when replacing fishmeal by plant protein sources. In the second group, the diets were contained 20 (D5), 25 (D6), and 30% (D7) without AAs. The best growth performance was achieved in fish fed with diet D1. Total feed intake was increased with an increase in dietary protein level with AAs. The specific growth rate showed a similar pattern with a significant difference between control, D4 and D7 compared to other groups. The feed conversion ratio decreased when protein levels in the diets increased. The protein efficiency ratio showed a similar performance, with a slight increase between the control diet and diets with AAs. However, insignificant differences (P>0.05) were observed between diets with and without AAs. An economic evaluation indicated that inclusion of low fishmeal in tilapia diets reduced the price/kg of diets compared to control.Este estudo foi realizado para determinar uma substituição adequada de farinha de peixe com aminoácidos (AA) e níveis otimizados de proteína em dietas práticas para Oreochromis niloticus com peso corporal inicial médio de 12,52 ± 0,63 g. Seis dietas experimentais e controle (total de 7 dietas) divididas em dois grupos, e uma dieta controle (D1) contendo 32% de proteína. O primeiro grupo continha três dietas que incluíam diferentes níveis de proteína na dieta, viz. 20 (D2), 25 (D3) e 30% (D4) com AAs ao substituir a farinha de peixe por fontes de proteína vegetal. No segundo grupo, as dietas continham 20 (D5), 25 (D6) e 30% (D7) sem AAs. O melhor desempenho de crescimento foi alcançado em peixes alimentados com dieta D1. O consumo total de ração foi aumentado com o aumento do nível de proteína na dieta com AAs. A taxa de crescimento específico mostrou um padrão semelhante com uma diferença significativa entre o controle, D4 e D7 em comparação com outros grupos. A taxa de conversão alimentar diminuiu quando os níveis de proteína nas dietas aumentaram. A taxa de eficiência protéica apresentou desempenho semelhante, com discreto aumento entre a dieta controle e as dietas com AAs. Entretanto, diferenças insignificantes (P> 0,05) foram observadas entre dietas com e sem AAs. Uma avaliação econômica indicou que a inclusão de farinha de peixe baixa em dietas de tilápia reduziu o preço / kg de dietas em relação ao controle.Palavras-chave: suplementação de aminoácidos, avaliação econômica, desempenho de crescimento, ingredientes vegetais.Abdel-Warith, A-W et al.

show abstract

Hic74, a novel alanine and glycine rich matrix protein related to nacreous layer formation in the mollusc Hyriopsis cumingii

Cited by 13 publications

References 21 publications

'Palaeoshellomics’ reveals the use of freshwater mother-of-pearl in prehistory

'Palaeoshellomics’ reveals the use of freshwater mother-of-pearl in prehistory

Shell palaeoproteomics: First application of peptide mass fingerprinting for the rapid identification of mollusc shells in archaeology

The effect of replacement of fish meal with Amino Acids and Optimized Protein Levels in the diet of the Nile Tilapia Oreochromis niloticus

Contact Info

Product

Resources

About