“…In addition AmphiTcf includes a CRARF domain (amino acids 392-396) comparable to those of all known TCF/LEF proteins of invertebrates as well as Tcf1 and Tcf4 of vertebrates; this motif evidently both binds DNA and transactivates (Hecht and Stemmler, 2003). There is also a putative Groucho (Grg) binding domain (amino acids 212-241; exon VI), which is structurally similar to the Grg binding/ HIC-5 interaction site known for several splice variants of vertebrate TCF/LEF family members (Ghogomu et al, 2006). In contrast, AmphiTcf lacked counterparts for following motifs: the CAD domain of vertebrate Lef1, the CtBP domain of vertebrate Tcf3 and Tcf4, and the LVPQ and SXXSS domains of vertebrate Tcf3 (van Noort and Clevers, 2002;Liu et al, 2005).…”