Hibiscus sabdariffa Linn fruit derivatives as alternative agents for diabetes mellitus care: A basic insight

Abstract

This study discusses the effectiveness of methanolic extract and fractions (butanol, ethyl acetate, and n-hexane) of H. sabdariffa Linn fruit towards antidiabetic activities (in vitro). In order to test the efficacy, toxicity and insulin secretion capacity of rat pancreatic β-cell lines (BRIN-BD11) were tested with the methanolic extract and fractions. The outcomes showed that both the extract and the fractions demonstrated significantly lower levels of cytotoxic activities. Furthermore, the methanolic extract and fractions displayed varied sensitivity levels towards insulin release after an incubation period of 30 min. The methanolic extract, at a concentration of 300 µg/mL, significantly stimulated secretion of insulin by 2.85-fold (p<0.001). In addition, butanol, ethyl acetate, and n-hexane fractions revealed a gradual increase in insulin secretion. The stimulated insulin secretion for these fractions had been recorded at 2-fold (p<0.01), 2.67-fold, and 2.31-fold (p<0.001), respectively, at the highest concentrations. The methanolic extract and fractions also appeared to stimulate secretion of insulin with all modulators present, for example, potassium chloride (KCl), insulin secretion inhibitor (verapamil and diazoxide), as well as insulin secretagogue (tolbutamide and isobutylmethylxanthine (IBMX)). These results indicate that H. sabdariffa Linn fruit methanolic extract and fractions could indeed be beneficial for future development of antidiabetic drugs.

Hibiscus sabdariffa in Diabetes Prevention and Treatment—Does It Work? An Evidence-Based Review

“…The aim of the work is a review of the literature describing the use of roselle in the treatment of diabetes mellitus and its accompanying diseases. Roselle has been the subject of many original and reviewed papers, including conditions that may be associated with diabetes and metabolic syndrome [ 35 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 ]. However, the existing works are older or incompletely related to diabetes, and research progress on this topic is very large [ 69 , 70 , 71 , 72 ].…”

“…These abilities are attributed to fibroblast stimulation as well as regulation and coordination of wound vascularization by methanolic extracts from roselle fruit [ 98 ]. Also, methanolic extracts and fractions of various solvents (butanol, ethyl acetate, and n-hexane) prepared with roselle fruit were tested with regard to insulin secretion stimulation and glucose uptake [ 62 , 99 ]. The studies performed on rat pancreatic β-cell BRIN-BD11 line showed an indirect hypoglycemic effect of fruit methanolic extracts and fractions by stimulating insulin secretion in these cells, but n-hexane and butanol fractions exhibited a higher cytotoxic effect on the tested cells than methanolic extract or ethyl acetate fraction [ 62 ].…”

“…Also, methanolic extracts and fractions of various solvents (butanol, ethyl acetate, and n-hexane) prepared with roselle fruit were tested with regard to insulin secretion stimulation and glucose uptake [ 62 , 99 ]. The studies performed on rat pancreatic β-cell BRIN-BD11 line showed an indirect hypoglycemic effect of fruit methanolic extracts and fractions by stimulating insulin secretion in these cells, but n-hexane and butanol fractions exhibited a higher cytotoxic effect on the tested cells than methanolic extract or ethyl acetate fraction [ 62 ]. Glucose uptake by 3T3F442A adipocytes and regulation of glucose transporter 4 (GLUT4) in L6 myotube cells was also assessed for the aforementioned fruit extracts [ 99 ].…”

“…The glucose uptake by 3T3F442A adipocytes was stimulated by methanolic extract and fractions prepared from Hibiscus sabdariffa fruits, but these preparations did not have a significant impact on GLUT4 distribution on L6 myotubes surface [ 99 ]. The authors conclude that the fruits of roselle provide a promising basis for the synthesis of a new generation of antidiabetic drugs ( Figure 2 ) [ 62 ]. Sunmonu et al [ 100 ] confirmed the anti-diabetic properties of another part of the roselle plant.…”