Hibiscus sabdariffa anthocyanins-rich extract: Chemical stability, in vitro antioxidant and antiproliferative activities

Abstract

Hibiscus sabdariffa calyx is a rich source of anthocyanins and other bioactive compounds but no study reported the effects of experimental conditions on the extraction of these chemical compounds. Therefore, the effects of time and extraction temperature on the bioactive compounds and antioxidant activity of Hibiscus sabdariffa calyx were evaluated. In addition, the effects of copigmentation and pH on the stability of anthocyanins were assessed and the cytotoxic effects (LC, IC, and GC) of the extracts were determined in relation to tumor cell lines - Caco-2, HepG-2, HCT8, and A549. The temperature significantly influenced the total anthocyanins and flavonoids contents. The interaction between time/temperature influenced the total phenolic content and ascorbic acid. The t and the percentage of colour retention decreased markedly at temperatures above 80 °C. Variations in pH conserved the antioxidant activity of the anthocyanins, and the protonation-deprotonation process of the extract was reversible. The treatment of cells with purified anthocyanin extract or crude extracts at 5-800 μg mL did not show significant cytotoxic effects on the cell lines, corroborating the chemical antioxidant effect of the extracts (DPPH assay). Cyanidin-3-glucoside, delphinidin-3-sambubioside, delphinidin-3-glucoside, and cyanidin-3-sambubioside were identified in the extracts by LC-ESI-MS.

Red Chicory (Cichorium intybus) Extract Rich in Anthocyanins: Chemical Stability, Antioxidant Activity, and Antiproliferative Activity In Vitro

“…This value is close to that observed by Maciel et al. () for H. sabdariffa L. calyx extract (89.6 kJ/mol). High activation energy values suggest that the compounds present in an extract have good temperature stability; the higher the activation energy, the slower the anthocyanin degradation reaction (Atkins & Jones, ).…”

“…Our thermal stability results for red chicory extract were similar to those found by Maciel et al. () for Hibiscus sabdariffa L. extract. The half‐life was 41.2 hr at 60 °C and 1.5 hr at 100 °C; the rate constants at these temperatures were 2.83 × 10 −4 and 7.52 × 10 −3 min −1 , respectively.…”

“…The stability of the anthocyanins in relation to increasing temperature was evaluated according to the methods of Reyes and Cisneros‐Zevallos () and Maciel et al. (). A solution was prepared using the extract with the highest anthocyanin content in sodium citrate/citric acid buffer at pH 3.0.…”

Estudo Químico E Biológico Do Extrato Aquoso Hibiscus Sabdariffa (Malvaceae)

“…Tal fator pode ser explicado devido ao poder extrator do solvente etanólico ter possibilitado a extração de uma maior quantidade de compostos bioativos. Além disso, a literatura cita que os valores da atividade antioxidante em extratos de H. sabdariffa variam de acordo com a origem geográfica da planta(ZHEN et al, 2016), processos de adubação(RAMOS et al, 2011) e métodos de extração(MACIEL et al, 2018). Logo, não podemos descartar a ação destes fatores abióticos na composição do extrato.…”

AVALIAÇÃO IN VITRO DE ÓLEOS ESSENCIAIS DE COPAÍBA, BURITI E TUCUMÃ PARA CONTROLAR Sthapylococcus aureus

“…Tal fator pode ser explicado devido ao poder extrator do solvente etanólico ter possibilitado a extração de uma maior quantidade de compostos bioativos. Além disso, a literatura cita que os valores da atividade antioxidante em extratos de H. sabdariffa variam de acordo com a origem geográfica da planta(ZHEN et al, 2016), processos de adubação (RAMOS et al, 2011) e métodos de extração(MACIEL et al, 2018). Logo, não podemos descartar a ação destes fatores abióticos na composição do extrato.…”