Hibiscus acid from Hibiscus sabdariffa (Malvaceae) has a vasorelaxant effect on the rat aorta

Zheoat, Ahmed M.; Gray, Alexander I.; Igoli, John O.; Ferro, Valerie A.; Drummond, Robert M.

doi:10.1016/j.fitote.2019.01.012

Cited by 38 publications

(31 citation statements)

References 51 publications

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“…In accordance with previous work that studied hibiscus acid, which is a water extract component of H. sabdariffa, on aorta [22], our results showed that there was no significant difference in relaxation achieved in either endothelium-intact or endothelium-denuded vessels. Therefore, the relaxation of mesenteric artery rings to H. sabdariffa AF seems to be endothelium-independent.…”

Section: Discussionsupporting

confidence: 93%

“…Several hypotheses have been suggested to explain the mechanism(s) involved in the reduction of arterial blood pressure, such as angiotensin-converting enzyme inhibition, the diuretic effect, or the direct vasorelaxant effect [21], but none have been clearly established. In some studies, it has been suggested that the relaxation is either endothelium dependent and/or endothelium independent due to inhibition of Ca 2+ influx or activation of K + channels [14,16,22], but no electrophysiological study has been performed to bring direct evidence.…”

Section: Introductionmentioning

confidence: 99%

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Aqueous Fraction from Hibiscus sabdariffa Relaxes Mesenteric Arteries of Normotensive and Hypertensive Rats through Calcium Current Reduction and Possibly Potassium Channels Modulation

et al. 2020

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Background/Objectives: Hibiscus sabdariffa L. (H. sabdariffa (HS)) extract has a vascular relaxant effect on isolated rat thoracic aorta, but data on small resistance arteries, which play an important role on the development of hypertension, are still missing. The purposes of this study were (1) to assess the effect on isolated mesenteric arteries (MA) from normotensive (Wistar and Wistar-Kyoto (WKY)) and spontaneous hypertensive rats (SHR); (2) to elucidate the mechanism(s) of action underling the relaxant effect in light of bioactive components. Methods: Vascular effects of HS aqueous fraction (AF) on isolated MA rings, as well as its mechanisms of action, were assessed using the contractility and intracellular microelectrode technique. The patch clamp technique was used to evaluate the effect of HS AF on the L-type calcium current. Extraction and enrichment of AF were carried out using liquid–liquid extraction, and the yield was analyzed using HPLC. Results: The HS AF induced a concentration-dependent relaxant effect on MA rings of SHR (EC50 = 0.83 ± 0.08 mg/mL), WKY (EC50 = 0.46 ± 0.04 mg/mL), and Wistar rats (EC50 = 0.44 ± 0.08 mg/mL) pre-contracted with phenylephrine (10 µM). In Wistar rats, the HS AF maximum relaxant effect was not modified after endothelium removal or when a guanylate cyclase inhibitor (ODQ, 10 µM) and a selective β2-adrenergic receptor antagonist (ICI-118551, 1 µM) were incubated with the preparation. Otherwise, it was reduced by 34.57 ± 10.66% when vascular rings were pre-contracted with an 80 mM [K+] solution (p < 0.001), which suggests an effect on ionic channels. HS AF 2 mg/mL significantly decreased the peak of the L-type calcium current observed in cardiac myocytes by 24.4%. Moreover, though the vasorelaxant effect of HS, AF was reduced by 27% when the nonselective potassium channels blocker (tetraethylammonium (TEA) 20 mM) was added to the bath (p < 0.01). The extract did not induce a membrane hyperpolarization of smooth muscle cells, which might suggest an absence of a direct effect on background potassium current. Conclusion: These results highlight that the antihypertensive effect of HS probably involves a vasorelaxant effect on small resistance arteries, which is endothelium independent. L-type calcium current reduction contributes to this effect. The results could also provide a link between the vasorelaxant effect and the bioactive compounds, especially anthocyanins.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Aqueous Fraction from Hibiscus sabdariffa Relaxes Mesenteric Arteries of Normotensive and Hypertensive Rats through Calcium Current Reduction and Possibly Potassium Channels Modulation

et al. 2020

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“…The results are similar to those obtained by related lavonoid glycosides [7]. Kinetic determinations suggested that these compounds inhibit the enzyme activity by competing with the substrate for the active site [8].…”

Section: Introductionsupporting

confidence: 85%

Determination of anthocyanin content in two varieties of Hibiscus Sabdariffa from Selangor, Malaysia using a combination of chromatography and spectroscopy

Choong¹,

Yousof²,

Jamal³

et al. 2019

J Plant Sci Phytopathol

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“…Probably, this scarce scientific attention is attributed to the fact that hibiscus acid is not commercially available and is a chiral compound and diastereomer of garcinia acid (extracted from Garcinia cambogia); which is commercially available. Practically, the most representative evidence on their extraction, properties and/or chemical characteristics have been analyzed by Zheoat et al, (2019) [37] and Portillo-Torres et al, (2019) [69]; who mention that their empirical formula is C6H6O7•H2O. Through crystallographic analysis and X-ray spectroscopy confirmed that HA is a five-membered lactone ring (similar to HCA), with four carbon atoms and one oxygen atom.…”

Section: Hibiscus Acidmentioning

confidence: 99%

“…Regarding its antihypertensive effect, Zheoat et al, (2019) [37] conducted a comparative study between a crude extract of Hibiscus sabdariffa and the HA derived from the same extract on the direct vasorelaxant effect in the Sprague-Dawley rat aorta. At the end of the study, they confirmed that HA was more potent and effective, attributing its vasorelaxant action to the inhibition of Ca 2+ influx via voltage-dependent Ca 2+ channels (an identical mechanism observed in garcinia acid; Ha diastereoisomer).…”

Section: Hibiscus Acidmentioning

confidence: 99%

Organic Acids from Roselle (Hibiscus sabdariffa L.)—A Brief Review of Its Pharmacological Effects

et al. 2020

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Roselle (Hibiscus sabdariffa L.), also known as jamaica in Spanish, is a perennial plant that grows in tropical and subtropical regions, including China, Egypt, Indonesia, Mexico, Nigeria, Thailand, and Saudi Arabia. It has a long history of uses, mainly focused on culinary, botanical, floral, cosmetic, and medicinal uses. The latter being of great impact due to the diuretic, choleretic, analgesic, antitussive, antihypertensive, antimicrobial, immunomodulatory, hepatoprotective, antioxidant, and anti-cancer effects. These therapeutic properties have been attributed to the bioactive compounds of the plant, mainly phenolic acids, flavonoids, anthocyanins, and organic acids (citric, hydroxycitric, hibiscus, tartaric, malic, and ascorbic). Most literature reviews and meta-analyses on the therapeutic potential of Hibiscus sabdariffa L. (Hs) compounds have not adequately addressed the contributions of its organic acids present in the Hs extracts. This review compiles information from published research (in vitro, in vivo, and clinical studies) on demonstrated pharmacological properties of organic acids found in Hs. The intent is to encourage and aid researchers to expand their studies on the pharmacologic and therapeutic effects of Hs to include assessments of the organic acid components.

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Hibiscus acid from Hibiscus sabdariffa (Malvaceae) has a vasorelaxant effect on the rat aorta

Cited by 38 publications

References 51 publications

Aqueous Fraction from Hibiscus sabdariffa Relaxes Mesenteric Arteries of Normotensive and Hypertensive Rats through Calcium Current Reduction and Possibly Potassium Channels Modulation

Aqueous Fraction from Hibiscus sabdariffa Relaxes Mesenteric Arteries of Normotensive and Hypertensive Rats through Calcium Current Reduction and Possibly Potassium Channels Modulation

Determination of anthocyanin content in two varieties of Hibiscus Sabdariffa from Selangor, Malaysia using a combination of chromatography and spectroscopy

Organic Acids from Roselle (Hibiscus sabdariffa L.)—A Brief Review of Its Pharmacological Effects

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