HHIPL-1 (rs2895811) gene polymorphism is associated with cardiovascular risk factors and cardiometabolic parameters in Mexicans patients with myocardial infarction

Rodrı́guez-Pérez, José Manuel; Posadas-Sánchez, Rosalinda; Blachman‐Braun, Ruben; Vargas‐Alarcón, Gilberto; Posadas‐Romero, Carlos; Rodríguez-Cortés, Adrián Asael; López-Bautista, Fabiola; Tovilla-Zárate, Carlos Alfonso; Rojas-Toledo, Emma Xochitl; Borgonio-Cuadra, Verónica Marusa; Pérez-Hernández, Nonanzit

doi:10.1016/j.gene.2018.04.030

Cited by 10 publications

(10 citation statements)

References 39 publications

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“…In order to confirm that population stratification was not a bias or a confounding factor in the study, we previously determined a panel of 265 ancestry informative markers (AIMs) distinguishing Amerindian, European, and African ancestry. After performing this ancestry analysis (Rodríguez-Pérez et al, 2018[ 21 ]; Posadas-Sánchez et al, 2017[ 18 ]), the results showed a similar distribution in the AIMs frequencies with no statistical significance between the study groups.…”

Section: Methodsmentioning

confidence: 99%

“…As part of the GEA Study Cohort, the clinical, demographic, anthropometric, and metabolic variables, have been described in detail in our previous reports (Rodríguez-Pérez et al, 2018[ 21 ]; Posadas-Sánchez et al, 2017[ 17 ]).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, with the aim to establish the genetic associations of the different genotypes with the disease, we performed a multivariate logistic regression analysis considering six models: additive, dominant, recessive, heterozygote, co-dominant 1, and co-dominant 2. These inheritance models have been described previously (Rodríguez-Pérez et al, 2018[ 21 ]). All models were adjusted by age, gender, body mass index, smoking habit, and type 2 diabetes mellitus.…”

Section: Methodsmentioning

confidence: 99%

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A haplotype of the phosphodiesterase 4D (PDE4D) gene is associated with myocardial infarction and with cardiometabolic parameters: the GEA study

Rodrı́guez-Pérez

Posadas-Sánchez

Blachman‐Braun

et al. 2018

EXCLI Journal; 17:Doc1182; ISSN 1611-2156

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A haplotype of the phosphodiesterase 4D (PDE4D) gene is associated with myocardial infarction and with cardiometabolic parameters: the GEA study

Rodrı́guez-Pérez

Posadas-Sánchez

Blachman‐Braun

et al. 2018

EXCLI Journal; 17:Doc1182; ISSN 1611-2156

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“…5 Moreover, genetic factors are reported as vital participants in the development and progression of MI. 6 The ADIPOQ gene on the long arm of chromosome 3q27 encodes adiponectin and comprises three exons and two introns. 7 Adiponectin (ADIPOQ), a 30 kDa protein that originates mainly from adipose tissues, plays a vital role in glucose metabolism and enhances sensitivity to insulin.…”

Section: Introductionmentioning

confidence: 99%

<p>Role of Adiponectin Gene and Receptor Polymorphisms and Their mRNA Levels with Serum Adiponectin Level in Myocardial Infarction</p>

Saleh

Tayel

Shalaby

et al. 2020

TACG

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Background and Aim Genetic factors are vital participants in the development and progression of myocardial infarction (MI). Adiponectin has been assumed to have a protective role in MI and adiponectin receptors variants could be a determinant for atherosclerosis. We aimed to evaluate the prevalence of ADIPOQ (rs2241766) and ADIPOR2 (rs10773989) polymorphisms and their association with mRNA levels and circulatory adiponectin levels in patients with MI. Subjects and Methods A total of 220 participants were classified into two groups: group 1 included 120 patients with MI, and group 2 involved 100 healthy participants as controls. Genotyping of ADIPOQ (rs2241766) and ADIPOR2 (rs10773989) polymorphisms were analyzed using an allele discrimination assay with real-time PCR and their relative expression or mRNA levels were determined by real-time PCR. Serum adiponectin level was determined using an ELISA technique. Results The ADIPOQ rs2241766 GG genotype and G allele and the CC genotype and C allele of ADIPOR2 rs10773989 were significantly prevalent in patients with MI and associated with increased risk of MI. We detected a marked reduction in serum adiponectin, ADIPOQ and ADIPOR2 mRNA levels in patients than control. The GG genotype of ADIPOQ rs2241766 and the CC genotype of ADIPOR2 rs10773989 had the lowest levels of their mRNA and adiponectin level in both patients and controls. Conclusion Adiponectin gene and receptor variants are potentially related to MI risk; furthermore, their expressions were markedly depressed in MI which suggests their use as potential biomarkers for MI.

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“…Serum lipids, including total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol, and triglycerides, are important clinical diagnostic indices of cardiovascular diseases [1]. Despite progress in the treatment of abnormal levels of cholesterol and other lipids, cardiometabolic diseases represent prominent causes of human morbidity and mortality [2]. Pigs are widely used in biomedical research [3,4].…”

Section: Introductionmentioning

confidence: 99%

Relationship between porcine miR-20a and its putative target low-density lipoprotein receptor based on dual luciferase reporter gene assays

Ding

Zhu

et al. 2019

Asian-Australas J Anim Sci

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Objective Mutations in low-density lipoprotein receptor ( LDLR ), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC -miR-20a. Methods Bioinformatic analysis predicted that porcine LDLR is a target of SSC -miR-20a. Wild-type and mutant LDLR 3′-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3′-UTR and pGL3 Control LDLR mutant-3 ′ -UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine pre-miR-20a-coding sequence between the Hind III and Bam HI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3′-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR-3′-UTR-driven (p<0.01), but not mutant LDLR-3′-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = −0.656, p<0.05). Conclusion LDLR is a potential target of miR-20a, which might directly bind the LDLR 3′-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.

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HHIPL-1 (rs2895811) gene polymorphism is associated with cardiovascular risk factors and cardiometabolic parameters in Mexicans patients with myocardial infarction

Cited by 10 publications

References 39 publications

A haplotype of the phosphodiesterase 4D (PDE4D) gene is associated with myocardial infarction and with cardiometabolic parameters: the GEA study

A haplotype of the phosphodiesterase 4D (PDE4D) gene is associated with myocardial infarction and with cardiometabolic parameters: the GEA study

<p>Role of Adiponectin Gene and Receptor Polymorphisms and Their mRNA Levels with Serum Adiponectin Level in Myocardial Infarction</p>

Relationship between porcine miR-20a and its putative target low-density lipoprotein receptor based on dual luciferase reporter gene assays

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