Hfq-Assisted RsmA Regulation Is Central to Pseudomonas aeruginosa Biofilm Polysaccharide PEL Expression

Irie, Yasuhiko; Mensa, Agnese La; Murina, Victoriia; Hauryliuk, Vasili; Tenson, Tanel; Shingler, Victoria

doi:10.3389/fmicb.2020.482585

Cited by 13 publications

(11 citation statements)

References 98 publications

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“…The above results fully demonstrated that Hfq can play a pivotal role of comprehensively regulating the physiological activities of Bt. Hfq had also been shown to regulate bacterial motility ( Schachterle et al, 2019 ), biofilm formation ( Irie et al, 2020 ), and virulence in some Gram-negative bacteria ( Pearl Mizrahi et al, 2021 ), suggesting that the regulation of certain physiological activities in bacteria by Hfq is universal. However, in Bs, deletion of Hfq was found to affect relatively fewer physiological functions, such as bacterial survival ( Rochat et al, 2015 ), motility, chemotaxis ( Jagtap et al, 2016 ) and cellulolysis etc.…”

Section: Discussionmentioning

confidence: 99%

The RNA Chaperone Protein Hfq Regulates the Characteristic Sporulation and Insecticidal Activity of Bacillus thuringiensis

Zhang

et al. 2022

Front. Microbiol.

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Bacillus thuringiensis (Bt) is one of the most widely used bio-insecticides at present. It can produce many virulence factors and insecticidal crystal proteins during growth and sporulation. Hfq, on the other hand, is a bacterial RNA chaperone that can regulate the function of different kinds of RNAs, thereby affecting various bacterial phenotypes. To further explore the physiological functions of Hfq in Bt, we took BMB171 as the starting strain, knocked out one, two, or three hfq genes in its genome in different combinations, and compared the phenotypic differences between the deletion mutant strains and the starting strain. We did observe significant changes in several phenotypes, including motility, biofilm formation, sporulation, and insecticidal activity against cotton bollworm, among others. Afterward, we found through transcriptome studies that when all hfq genes were deleted, 32.5% of the genes in Bt were differentially transcribed, with particular changes in the sporulation-related and virulence-related genes. The above data demonstrated that Hfq plays a pivotal role in Bt and can regulate its various physiological functions. Our study on the regulatory mechanism of Hfq in Bt, especially the mining of the regulatory network of its sporulation and insecticidal activity, could lay a theoretical foundation for the better utilization of Bt as an effective insecticide.

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Section: Discussionmentioning

confidence: 99%

The RNA Chaperone Protein Hfq Regulates the Characteristic Sporulation and Insecticidal Activity of Bacillus thuringiensis

Zhang

et al. 2022

Front. Microbiol.

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“…Indeed, it has been thoroughly characterized in T3SS + strains where it activates the T3SS. Vfr regulates its own expression through a positive feedback loop (Fuchs et al, 2010), and is under post-transcriptional regulation by the RNA-binding proteins RsmA and Hfq (Irie et al, 2020). Hfq is a regulatory protein that mainly facilitates regulation of translation via interaction with small noncoding RNAs and their mRNA targets (Vogel & Luisi, 2011).…”

Section: Signal Functionmentioning

confidence: 99%

The regulation of bacterial two‐partner secretion systems

Trouillon

Attrée

Elsen

2023

Molecular Microbiology

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Two‐partner secretion (TPS) systems, also known as Type Vb secretion systems, allow the translocation of effector proteins across the outer membrane of Gram‐negative bacteria. By secreting different classes of effectors, including cytolysins and adhesins, TPS systems play important roles in bacterial pathogenesis and host interactions. Here, we review the current knowledge on TPS systems regulation and highlight specific and common regulatory mechanisms across TPS functional classes. We discuss in detail the specific regulatory networks identified in various bacterial species and emphasize the importance of understanding the context‐dependent regulation of TPS systems. Several regulatory cues reflecting host environment during infection, such as temperature and iron availability, are common determinants of expression for TPS systems, even across relatively distant species. These common regulatory pathways often affect TPS systems across subfamilies with different effector functions, representing conserved global infection‐related regulatory mechanisms.

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“…Consequently, in P. aeruginosa , the Rsm system influences organism pathogenicity and patient disease state by governing the transition from acute to chronic infection ( Sonnleitner et al., 2006 ). Biofilm formation is further controlled in P. aeruginosa by the chaperone protein Hfq, which mediates the expression of RsmA protein in this network and thus indirectly controls the formation of biofilms ( Irie et al., 2020 ).…”

Section: Impacts Of Hfq-srna Regulation On Bacterial Cell Physiology and Virulencementioning

confidence: 99%

Impacts of Small RNAs and Their Chaperones on Bacterial Pathogenicity

Djapgne

Oglesby-Sherrouse

2021

Front. Cell. Infect. Microbiol.

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Bacterial small RNAs (sRNAs) are critical post-transcriptional regulators that exert broad effects on cell physiology. One class of sRNAs, referred to as trans-acting sRNAs, base-pairs with mRNAs to cause changes in their stability or translation. Another class of sRNAs sequesters RNA-binding proteins that in turn modulate mRNA expression. RNA chaperones play key roles in these regulatory events by promoting base-pairing of sRNAs to mRNAs, increasing the stability of sRNAs, inducing conformational changes on mRNA targets upon binding, or by titrating sRNAs away from their primary targets. In pathogenic bacteria, sRNAs and their chaperones exert broad impacts on both cell physiology and virulence, highlighting the central role of these systems in pathogenesis. This review provides an overview of the growing number and roles of these chaperone proteins in sRNA regulation, highlighting how these proteins contribute to bacterial pathogenesis.

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Hfq-Assisted RsmA Regulation Is Central to Pseudomonas aeruginosa Biofilm Polysaccharide PEL Expression

Cited by 13 publications

References 98 publications

The RNA Chaperone Protein Hfq Regulates the Characteristic Sporulation and Insecticidal Activity of Bacillus thuringiensis

The RNA Chaperone Protein Hfq Regulates the Characteristic Sporulation and Insecticidal Activity of Bacillus thuringiensis

The regulation of bacterial two‐partner secretion systems

Impacts of Small RNAs and Their Chaperones on Bacterial Pathogenicity

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