2006
DOI: 10.1002/lsm.20416
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Hexyl‐aminolevulinate‐mediated photodynamic therapy: How to spare normal urothelium. An in vitro approach

Abstract: Background and Objectives: Photodynamic therapy (PDT) of superficial bladder cancer may cause damages to the normal surrounding bladder wall. Prevention of these is important for bladder healing. We studied the influence of photosensitizer concentration, irradiation parameters, and production of reactive oxygen species (ROS) on the photodynamically induced damage in the porcine urothelium in vitro. The aim was to determine the threshold conditions for the cell survival. Methods: Living porcine bladder mucosae … Show more

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Cited by 6 publications
(5 citation statements)
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References 39 publications
(39 reference statements)
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“…This suggests that there is a light dose threshold for a photodynamic reaction to cause cell death using Ce6-PVP. As addressed by many researchers, the interdependence of photosensitizer concentration, route of administration, and incubation time determines the irradiance and radiant exposure required to cause PDT-induced tissue damage [26][27][28]. As such, a cautious extrapolation of PDT parameters obtained from the CAM model may provide a useful guide to establish a basis for the clinical use of Ce6-PVP for bladder cancer.…”
Section: Ce6mentioning
confidence: 98%
“…This suggests that there is a light dose threshold for a photodynamic reaction to cause cell death using Ce6-PVP. As addressed by many researchers, the interdependence of photosensitizer concentration, route of administration, and incubation time determines the irradiance and radiant exposure required to cause PDT-induced tissue damage [26][27][28]. As such, a cautious extrapolation of PDT parameters obtained from the CAM model may provide a useful guide to establish a basis for the clinical use of Ce6-PVP for bladder cancer.…”
Section: Ce6mentioning
confidence: 98%
“…Protoporphyrin IX (PPIX) is known as a precursor to haem. Haem itself is not a photosensitiser, due to the coordination of a paramagnetic ion in the centre of the macrocycle, causing significant reduction in excited state lifetimes [ 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 ].…”
Section: Photodynamic Therapy In Bladder Cancermentioning
confidence: 99%
“…On the other hand, photosensitizers obtained from natural sources and having a positive effect are hypericin [ 57 , 58 , 59 ] and chlorophyllin [ 60 , 61 ]. Photosensitizers should absorb light in the red or far-red wavelengths in order to penetrate tissue deeper [ 49 , 50 , 51 , 52 , 53 , 54 , 62 ].…”
Section: Photodynamic Therapy In Bladder Cancermentioning
confidence: 99%
“…7- 18 The effect of illumination was examined in rat ovarian cancer and porcine bladder mucosa. 19,20 Moreover, tissue response to HAL-PDT was monitored in rat bladder. The results revealed a clear treatment response in vivo including decreased tissue oxygenation and immunoprecipitated with anti-DNP antibody prior to 2D-GE and MS. 31,32…”
Section: Introductionmentioning
confidence: 99%